Conjugation in Paramecium bursaria. II. Nuclear phenomena in lethal conjugation between varieties

Chen, Tze-Tuan

doi:10.1002/jmor.1050790202

Cited by 25 publications

(4 citation statements)

References 6 publications

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“…This kind of hybrid conjugation closely resembles that occurring in mixtures of syngen 2 and syngen 4 of Paramecium bursaria (JENNINGS et al 1944, CHEN 1946. There is, however, a remarkable difference between the two phenomena: in the present case, a unilateral induction of selfing is observed, which is lacking in the case of P. bursaria.…”

Section: Received: 27th May 1964supporting

confidence: 78%

On Conjugation BetweenEuplotes VannusO. F. Mueller andEuplotes MinutaYocom

Nobili

1964

Caryologia

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Section: Received: 27th May 1964supporting

confidence: 78%

On Conjugation BetweenEuplotes VannusO. F. Mueller andEuplotes MinutaYocom

Nobili

1964

Caryologia

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“…. "; studying the cytology of conjugation between 2 varieties whose representatives had come from different parts of the world, Chen (7) found that micronuclear abnormalities developed late in the crescent stage (17 hours after the start of conjugation) so that the 1st prezygotic division was not completed and pronuclei *This research was supported by grants G-22076 and GB-3289 from the National Science Foundation.…”

Section: Wealth Of Genetic Information Has Come From Studiesmentioning

confidence: 99%

The Exchange of RNA and Protein During Conjugation in Tetrahymena*

McDonald

1966

The Journal of Protozoology

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SYNOPSIS. Exchange of cytoplasm in Tetrahymena pyriformis, syngen 1, has been demonstrated by growing cells of 1 mating type in medium supplemented with H3‐uridine or H3‐histidine, washing, mixing with cells of an unlabeled, starved mating type, sampling conjugants at different times, and preparing autoradiographs. It was found that cytoplasmic interchange begins soon after the mates unite, and has become extensive before the end of the 1st prezygotic prophase (micronuclear crescent stage). When the RNA in one mating type had been labeled with H3‐uridine, the activity was distributed almost evenly between the mates by late stages of conjugation. These results are consistent with electron micrographs of this syngen showing small pores in the attachment region of the mates, and many free ribosomes in the cytoplasm (8,11). By contrast, when protein in one mating type had been labeled with H3‐histidine, these cells at late conjugation remained about twice as active as their originally unlabeled mates, presumably because of the physical characteristics of some structures which incorporated the amino acid (for example, cilia and membranes of the cell surface; cytoplasmic bodies, such as mitochondria, larger than the pores). That the radioactivity in the originally unlabeled cells came from their mates and not from the environment is indicated by the continued presence of inactive non‐conjugants after 1 and 2 days in the mating type mixtures. Other cells which did acquire small amounts of active cytoplasm probably had engaged in abortive conjugation, separating from labeled mates before forming and exchanging pronuclei.

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“…51 VII killers X 131 XVI sensitives tion, with the animal of complementary mating type (and the other variety) in intervarietal pairs, will also survive. Chen (1946a) reported unions of three animals in P. bursaria and showed that two conjugate while the third, which may attach in any position to the conjugant of complementary mating type, undergoes autogamy. In our work, of the 21 clones derived from seven unions of three animals in mixtures between stock 51 of variety 4 and stock 131 of variety 8, twenty survived and produced normal cultures of their respective varieties.…”

Section: The Results Of Conjugationmentioning

confidence: 99%

The Interaction of Nucleus and Cytoplasm in the Isolation and Evolution of Species of Paramecium

Levine¹

1953

Evolution

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Conjugation in Paramecium bursaria. II. Nuclear phenomena in lethal conjugation between varieties

Cited by 25 publications

References 6 publications

On Conjugation BetweenEuplotes VannusO. F. Mueller andEuplotes MinutaYocom

On Conjugation BetweenEuplotes VannusO. F. Mueller andEuplotes MinutaYocom

The Exchange of RNA and Protein During Conjugation in Tetrahymena*

The Interaction of Nucleus and Cytoplasm in the Isolation and Evolution of Species of Paramecium

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