Conjugation of carboxymethyl cellulose and dopamine for cell sheet harvesting

Abstract

This manuscript focuses on the cell sheet preparation methodology with the conjugation of carboxymethylcellulose (CMC) and dopamine (DA).

Transplantation of human corneal limbal epithelial cell sheet harvested on synthesized carboxymethyl cellulose and dopamine in a limbal stem cell deficiency

“…CMC–DA was synthesized using a previously reported method (Hong et al, 2018; Sakai et al, 2009). Briefly, CMC (medium viscosity, Sigma) was dissolved in 50 mM of 4‐morpholineethanesulfonic acid (MES hydrate; Sigma) buffer (pH 5.5) with 1% (w/v) concentration for 12 h at room temperature (RT).…”

“…We recently introduced the conjugated carboxymethyl cellulose (CMC) with dopamine (DA) to conduct a simple coating with cellulose by DA bonding on the surface of the culture plate for cell sheet engineering (Hong et al, 2018). The surface coating process is a key strategy because cellulose detachment must be prohibited during the cell culture period until cell sheet harvesting with cellulase.…”

“…According to one recent study evaluating the feasibility of CMC–DA coating combined with cellulase enzymatic harvesting for human mesenchymal stem cells (HMSCs) or human corneal limbal epithelial (HCLE) cell sheet harvesting, both cell sheets showed a well‐preserved morphology and transparency (Hong et al, 2018). Moreover, Ko, Kato, and Iwata (2005) generated an endothelial cell sheet using a thin CMC coating with digestion of the coated cellulose with cellulase.…”

Advanced Bottom‐Up Engineering of Living Architectures

“…The poly( N ‐isopropylacrylamide) coating undergoes a sharp change in wettability from 37 to 32 °C, which spontaneously peels the cell sheet from the surface at room temperatures . Still, this one‐time use system is expensive, detachment time may vary significantly, and it can be difficult to handle due to the low range of temperature that triggers the peeling process . Thermally expandable hydrogel sheets or multilayered coatings are being explored as an alternative to this technology that facilitate transfer and stamping of cell sheets with spatially controlled cell adhesion .…”

“…More recently, Park and co‐workers embedded magnetic nanoparticles in thin hydrogel sheets for efficient harvesting of endothelial progenitor cell sheets . Using a bioinspired approach, researchers have also developed a cellulose‐dopamine coating which enabled cellulase‐assisted enzymatic harvesting with minimal cell damage as recently reported …”