Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion

Abstract

A surface resisting protein adsorption and cell adhesion is highly desirable for many biomedical applications such as diagnostic devices, biosensors and blood-contacting devices. In this study, a surface conjugated with sulfobetaine molecules was fabricated via the click reaction for the anti-fouling purpose. An alkyne-containing substrate (Alkyne-PPX) was generated by chemical vapor deposition of 4-ethynyl-[2,2]paracyclophane. Azide-ended mono-sulfobetaine molecules were synthesized and then conjugated on Alkyne-PPX via the click reaction. The protein adsorption from 10% serum was reduced by 57%, while the attachment of L929 cells was reduced by 83% onto the sulfobetaine-PPX surface compared to the protein adsorption and cell adhesion on Alkyne-PPX. In conclusion, we demonstrate that conjugation of monosulfobetaine molecules via the click chemistry is an effective way for reduction of non-specific protein adsorption and cell attachment.

Conjugation of Polysulfobetaine via Poly(pyrogallol) Coatings for Improving the Antifouling Efficacy of Biomaterials

“…Antifouling polymers are often highly hydrophilic polymers, such as polysaccharides, poly­(ethylene glycol) (PEG), and poly­(zwitterions), that adsorb a great amount of water to form a barrier to reduce nonspecific interactions between biomaterials and biological systems. , The coating methods for surface conjugation of antifouling polymers are generally categorized into “graft-to” and “graft-from” . “Graft-to” refers to physical or chemical immobilization of presynthesized polymers, − while “graft-from” refers to in situ polymerization of hydrophilic monomers from surface-bound initiators. − Some surface coating strategies, such as layer-by-layer (LbL) deposition, chemical vapor deposition (CVD) of functional films, and photoinitiated immobilization, could be applied to a wide range of substrates, but possess certain disadvantages. LbL deposition is usually tedious, time-consuming, and could not survive harsh sterilization conditions.…”