Conjugation of paclitaxel on adeno-associated virus (AAV) nanoparticles for co-delivery of genes and drugs

Wei, Fang; McConnell, Kellie I.; Yu, Tse-Kuan; Suh, Junghae

doi:10.1016/j.ejps.2012.02.022

Cited by 21 publications

(16 citation statements)

References 32 publications

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“…For example, amine-reactive taxol has already been conjugated to the amino groups of the AAV surface, as confirmed qualitatively by dot blot analysis. 23 The resulting taxol-AAV particles did not effectively eradicate cancer cells in vitro , possibly due to the limited drug conjugation and its low release from the virus. 23 PEGylated AAV particles via amine functionalities have also been developed to protect the virus from neutralization and enable significant levels of gene expression upon re-administration without compromising the patient's immune system.…”

Section: Introductionmentioning

confidence: 99%

Chemical modification of the adeno-associated virus capsid to improve gene delivery

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Chemical modification of the adeno-associated virus capsid to improve gene delivery

et al. 2020

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“…The role of the miRNA signature (5) and the expression of AAV membrane receptors (6–8) in the AAV cellular transmission are in the course of documentation. Discovering cellular mechanisms of rAAV transduction helps to understand the AAV biology and makes it possible to design new vectors-synthetic AAV mosaic serotypes (9), vectors with dsDNA (10), AAV chemo-conjugates (11). There is research indicating the possibilities of optimizing the efficiency of rAAV transduction by various physicochemical treatments.…”

Section: Introductionmentioning

confidence: 99%

Increased temperature‑related adeno‑associated virus vectors transduction of ovarian cancer cells ‑ essential signatures of AAV receptor and heat shock proteins

et al. 2019

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Recombinant adeno-associated viruses (rAAVs) are becoming more commonly used in clinical trials involving gene therapy. Additionally AAV-based drugs have already been registered. Gene therapy aims to increase transduction efficiency, increase in vivo selectivity and reduce side effects. One approach to achieve this is the use of physical factors, such as temperature or more specifically, hyperthermia, which is already utilized in oncology. The aim of the present study was to investigate the effect of hyperthermic conditions (40°C and 43°C) on the rAAV transduction efficiency of ovarian cancer cells (Caov-3 and NIH:OVCAR-3) and non-cancerous cells (AAV-293). The present study was designed to identify functional associations between the level of gene transfer and the expression of representative genes for rAAV transmission (AAVR (AAV receptor), heparan sulfate proteoglycan (HSPG) 1 and HSPG2) and heat shock proteins (HSPs). The expressions of selected genes were measured via reverse transcription-quantitative PCR and cell adhesion/invasion chamber tests were also performed. The results revealed that ovarian cancer cell lines were more efficiently transduced with rAAV vectors at an elevated temperature. Additionally, the expression patterns of AAVR, HSPG1 and HSPG2 genes were different between the tested lines. The expression of certain receptors in ascites-derived NIH:OVCAR-3 ovarian cancer cells was higher compared with tumor-derived Caov-3 cells at 37, 40 and 43°C, which indicates a higher transduction efficiency in the formerly mentioned cells. Ascites-derived ovarian cancer cells were characterized by high expressions of HSP40, HSP90 and HSP70 families. Lower levels of HSP expression were demonstrated in less-effectively transduced Caov-3 cells. Furthermore, expressions of the examined genes changed with increasing temperature. The results indicated that temperature-dependent transduction is associated with the expression of the rAAV receptor and HSP genes. The results of the current study may aid the design of effective protocols for ovarian cancer gene therapy.

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“…如果经过突变人为引入半胱氨酸, 可能导致二硫键的错配, 打乱病毒蛋白的正确折叠和自组装 [50] . 为了避免使用半胱氨酸-马来酰亚胺反应时形成二硫键, 可将衣壳蛋白表面袋或内部暴露的氨基酸突变为半胱氨酸, 这些图 1 腺相关病毒与紫杉醇的共价连接 [32] (网络版彩图)…”

Section: 病毒蛋白的半胱氨酸的巯基可与马来酰亚胺反应形成共价化学连接利用这个反应可将修饰分子偶联unclassified

Chemical modification strategies of viral vectors for gene therapy

Zhang¹,

Zhou²

2018

Sci. Sin.-Chim

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Modification of a viral satellite DNA-based gene silencing vector and its application to leaf or flower color change in Petunia hybrida Chinese Science Bulletin 51, 2208 (2006); Gene therapy for hemophilia B mediated by recombinant adeno-associated viral vector with hFIXR338A, a high catalytic activity mutation of human coagulation factor IX Science in China Series C-Life Sciences 44, 585 (2001); Targeting strategies for adeno-associated viral vector Chinese Science Bulletin 52, 1590 (2007); Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

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Conjugation of paclitaxel on adeno-associated virus (AAV) nanoparticles for co-delivery of genes and drugs

Cited by 21 publications

References 32 publications

Chemical modification of the adeno-associated virus capsid to improve gene delivery

Chemical modification of the adeno-associated virus capsid to improve gene delivery

Increased temperature‑related adeno‑associated virus vectors transduction of ovarian cancer cells ‑ essential signatures of AAV receptor and heat shock proteins

Chemical modification strategies of viral vectors for gene therapy

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