Conjugative Elements: Host Chromosome Function Modifiers

Shintani, Masaki; Takahashi, Yurika; Nojiri, Hideaki

doi:10.1007/978-4-431-54520-0_7

Cited by 2 publications

(2 citation statements)

References 125 publications

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“…Pmr, one of the three NAPs, binds many sites not only on pCAR1 but also on the host chromosome, and disruption of pmr was shown to have greater effects on the host transcriptome than pCAR1 carriage (Yun et al, 2010). Thus, new hosts phenotypes were achieved by the expression of not only genes on pCAR1 but also those on the host chromosome, and NAPs are key factors for their expression (Nojiri, 2013;Shintani et al, 2014). These studies, however, did not sufficiently analyse and compare the effects of the plasmid on these hosts because they were based only on transcriptome comparisons of the host cells at the early log phase.…”

Section: Introductionmentioning

confidence: 99%

Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1

Takahashi

Shintani

Takase

et al. 2014

Environmental Microbiology

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The impacts of plasmid carriage on the host cell were comprehensively analysed using the conjugative plasmid pCAR1 in three different Pseudomonas hosts, P. putida KT2440, P. aeruginosa PAO1 and P. fluorescens Pf0-1. Plasmid carriage reduced host fitness, swimming motility, and resistance to osmotic or pH stress. Plasmid carriage brought about alterations in primary metabolic capacities in the TCA cycle of the hosts. Differentially transcribed genes in the three hosts associated with plasmid carriage were identified by growth phase-dependent transcriptome analyses. Plasmid carriage commonly showed a greater effect on the host transcriptome at the transition and early stationary phases. The transcriptome alterations were similar between KT2440 and PAO1. Transcriptions of numbers of genes encoding ribosomal proteins, F-type ATPase, and RNAP core in both strains were not suppressed enough in the early stationary phase by plasmid carriage. These responses may have been responsible for the reduction in host fitness, motility and stress resistances. Host-specific responses to plasmid carriage were transcriptional changes of genes on putative prophage or foreign DNA regions. The extents of the impacts on host phenotypes and transcriptomes were similarly greatest in KT2440 and lowest in Pf0-1. These findings suggest that host cell function was actively regulated by plasmid carriage.

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Section: Introductionmentioning

confidence: 99%

Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1

Takahashi

Shintani

Takase

et al. 2014

Environmental Microbiology

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“…In our previous studies, we used the carbazole (CAR)‐degradative plasmid pCAR1 as a natural plasmid model because the molecular features and behaviour in various hosts have been investigated extensively (Nojiri, ; Shintani et al ., ). pCAR1 was originally isolated from Pseudomonas resinovorans CA10, which was isolated from activated sludge of a municipal wastewater treatment facility in Tokyo in 1993 (Ouchiyama et al ., ; Nojiri et al ., ) and was found to be a member of the incompatibility (Inc) P‐7 group that is transferred mainly among Pseudomonas genus (Shintani et al ., 2006; 2014b).…”

Section: Introductionmentioning

confidence: 98%

Effects of carbazole‐degradative plasmid pCAR1 on biofilm morphology in Pseudomonas putida KT2440

Lee

Takahashi

Oura

et al. 2016

Environ Microbiol Rep

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Bacteria typically form biofilms under natural conditions. To elucidate the effect of the carriage of carbazole-degradative plasmid pCAR1 on biofilm formation by host bacteria, we compared the biofilm morphology, using confocal laser scanning microscopy, of three pCAR1-free and pCAR1-carrying Pseudomonas hosts: P. putida KT2440, P. aeruginosa PAO1 and P. fluorescens Pf0-1. Although pCAR1 did not significantly affect biofilm formation by PAO1 or Pf0-1, pCAR1-carrying KT2440 became filamentous and formed flat biofilms, whereas pCAR1-free KT2440 formed mushroom-like biofilms. pCAR1 contains three genes encoding nucleoid-associated proteins (NAPs), namely, Pmr, Pnd and Phu. The enhanced filamentous morphology was observed in two double mutants [KT2440(pCAR1ΔpmrΔpnd) and KT2440(pCAR1ΔpmrΔphu)], suggesting that these NAPs are involved in modulating the filamentous phenotype. Transcriptome analyses of the double mutants identified 32 candidate genes that may be involved in filamentation of KT2440. Overexpression of PP_2193 in KT2440 induced filamentation and overexpression of PP_0308 or PP_0309 in KT2440(pCAR1) enhanced filamentation of cells over time. This suggests that pCAR1 induces development of an abnormal filamentous morphology by KT2440 via a process involving overexpression of several genes, such as PP_2193. In addition, pCAR1-encoded NAPs partly suppress too much filamentation of KT2440(pCAR1) by repressing transcription of some genes, such as PP_0308 and PP_0309.

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Conjugative Elements: Host Chromosome Function Modifiers

Cited by 2 publications

References 125 publications

Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1

Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1

Effects of carbazole‐degradative plasmid pCAR1 on biofilm morphology in Pseudomonas putida KT2440

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