Conjugative transposition of Tn916 and Tn925 in Bacillus popilliae

Dingman, Douglas W.

doi:10.1139/w99-036

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…1), it is plausible that the vanH , vanA , and vanX genes have been mobilized as a unit from another source and positioned into contact with appropriate control elements. Transfer of vancomycin resistance from an organism like P. popilliae to an enterococcus may first require transfer of a conjugative transposon from an enterococcus (or another Gram‐positive bacterium) to this organism, followed by insertion of vancomycin resistance determinants into the conjugative transposon, and thereafter transfer of the transposon and vancomycin resistance back into an enterococcus [38]. This may account for our difficulty in trying to transfer vancomycin resistance from P. popilliae into an enterococcus.…”

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confidence: 99%

Enterococcal-type glycopeptide resistance genes in non-enterococcal organisms

Patel

2000

FEMS Microbiology Letters

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Although the emergence of vancomycin-resistant enterococci can be attributed, in part, to the increasing use of vancomycin in clinical practice, and glycopeptide use in animal husbandry, the origins of the enterococcal vancomycin resistance genes are not clear. The vancomycin resistance-associated genes in Enterococcus gallinarum, Enterococcus casseliflavus/flavescens, Lactobacillus spp., Leuconostoc spp., Pediococcus spp., and Erysipelothrix rhusiopathiae, are not the source of the high-level vancomycin resistance-associated genes in enterococci. There are, however, environmental organisms which have been found to have gene clusters homologous to the enterococcal vanA, vanB and vanC gene clusters; these include the biopesticide Paenibacillus popilliae, and, to a lesser extent, the glycopeptide-producing organisms Amycolatopsis orientalis and Streptomyces toyocaensis. Still, the exact sources of the enterococcal vancomycin resistance genes remain a mystery.

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confidence: 99%

Enterococcal-type glycopeptide resistance genes in non-enterococcal organisms

Patel

2000

FEMS Microbiology Letters

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“…The diseaseencoding plasrnid from S. entomophila and S. proteamaculans has been readily conjugated between strains and species in the laboratory (Glare et al 1993). Conjugative transposons have also been shown to move into P. popilliae from Enterococcus faecalis (Andrewand Horder) Schleifer and Kilpper-Blaz and Bacillus subtilis (Ehrenberg) Cohn (Dingman 1999).…”

Section: Gene Transfermentioning

confidence: 99%

Environmental Impacts of Bacterial Biopesticides

Glare¹,

O’Callaghan²

2003

Environmental Impacts of Microbial Insecticides

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Functionality of Tn916 in Paenibacillus larvae

Dingman

2016

Arch Microbiol

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The conjugative transposon Tn916 was determined to be functional in Paenibacillus larvae in regard to expression of tetracycline resistance and conjugative transfer. Expression of erythromycin resistance, using Tn916ΔE, was also observed. Conjugative transfer experiments employing Paenibacillus popilliae strains Tc1001 and Em1001 as transposon donors and experiments using different P. larvae subspecies or different transposon-containing strains demonstrated interspecies and intraspecies transfer occurred for Tn916 and Tn916ΔE. Southern hybridization analysis of several Tn916-containing P. larvae isolates showed that the transposon randomly inserted into the bacterial chromosome with an indication that hot spot insertion had occurred. Hybridization analysis indicated single-copy insertion of Tn916 into the genome predominated. However, selection of multiple-resistant isolates (i.e., isolates containing Tn916 and Tn916ΔE) demonstrated that multiple copies of the transposon could coexist in the bacterial genome. Growth of transposon-containing isolates in broth medium in the absence of selective antibiotic pressure showed that Tn916 and Tn916ΔE were stably maintained in the bacterium.

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Conjugative transposition of Tn916 and Tn925 in Bacillus popilliae

Cited by 4 publications

References 31 publications

Enterococcal-type glycopeptide resistance genes in non-enterococcal organisms

Enterococcal-type glycopeptide resistance genes in non-enterococcal organisms

Environmental Impacts of Bacterial Biopesticides

Functionality of Tn916 in Paenibacillus larvae

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