Conjunctivitis Due to Chlamydophila felis (Chlamydia psittaci Feline Pneumonitis Agent) Acquired From a Cat: Case Report with Molecular Characterization of Isolates from the Patient and Cat

Hartley, John C.; Stevenson, Simon; Robinson, Angela; Littlewood, Jim; Carder, C; Cartledge, J; Clark, Carey; Ridgway, G.L.

doi:10.1053/jinf.2001.0845

Cited by 29 publications

(17 citation statements)

References 20 publications

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“…However, recently C. felis was isolated from a patient with non-C. trachomatis conjunctivitis and from one of the patient's cats (14). In addition, we also reported previously that 5.0% of small-animal clinic veterinarians were seropositive for C. felis (45).…”

supporting

confidence: 58%

Chlamydophila felisCF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis ofC. felisInfection

Ohya

Takahara

Kuroda

et al. 2008

Clin Vaccine Immunol

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Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.The chlamydiae are obligate intracellular bacterial pathogens, possessing a biphasic developmental cycle, consisting of a metabolically inactive infectious elementary body (EB) and a metabolically active noninfectious reticulate body. The bacteria within host cells occupy vacuoles termed inclusions. Chlamydiae cause a range of diseases in various animals, such as humans, birds, and cats. The family Chlamydiaceae is divided into two genera, Chlamydia and Chlamydophila (9). The genus Chlamydia comprises Chlamydia trachomatis (a human conjunctivitis and sexually transmitted disease agent), Chlamydia muridarum (a mouse pneumonia agent), and Chlamydia suis (a pig conjunctivitis agent). The latter genus, Chlamydophila, includes Chlamydophila pneumoniae (an agent for pneumonia and a suspected atherosclerosis agent), Chlamydophila psittaci (an agent for psittacosis), Chlamydophila abortus (a ruminant abortive agent), Chlamydophila caviae (isolated from guinea pigs), Chlamydophila pecorum (infecting ruminants), and Chlamydophila felis (infecting cats) (9).C. felis is a causative agent of feline chlamydiosis, which is characterized by acute and chronic conjunctivitis and pneumonia in cats (40). The prevalence of C. felis in cats with ocular signs or upper respiratory tract diseases (URTD) has been investigated by PCR or by detecti...

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supporting

confidence: 58%

Chlamydophila felisCF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis ofC. felisInfection

Ohya

Takahara

Kuroda

et al. 2008

Clin Vaccine Immunol

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“…C. psittaci was associated with chronic forms of follicular conjunctivitis and with ophthalmia neonatorum and was isolated from the nasal and conjunctival swabs of both humans and cats. 31,32 It was also shown that patients with ocular adnexal lymphoma have a higher prevalence of C. psittaci infection in both the tumor and the mononuclear cells in the peripheral blood, and that persistent C. psittaci infection may contribute to the development of these lymphomas, as supported by clinical responses to antibiotic therapy. 33 In the posterior segment, infection with C. pneumoniae has been associated with human choroidal neovascular membranes, suggesting that by inducing chronic inflammation and pro-angiogenic cytokines in the posterior segment of the eye, these species of Chlamydia may contribute to the pathogenesis of agerelated macular degeneration.…”

Section: Discussionmentioning

confidence: 99%

Clinical and Microbiological Diagnosis of Trachoma in Children Living in Rural Areas in the District of Attock, Punjab, Pakistan

Goldschmidt

Afghani²,

Nadeem³

et al. 2006

Ophthalmic Epidemiology

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“…In cattery B, one cat harbored genotype 1, one cat had genotype 3, one cat had genotype 11, and one cat had both genotypes 3 and 12. In cattery C, two cats harbored genotype 3 and seven cats had genotype 15.…”

Section: Selection Of Vntrs For Mlvamentioning

confidence: 99%

“…The risk of zoonotic transmission is presumably greater for immunocompromised individuals. C. felis infection has been associated with conjunctivitis and/or respiratory tract disease (4,15), community-acquired pneumonia (21), hepatosplenomegaly, glomerulonephritis, and endocarditis in humans (31).…”

mentioning

confidence: 99%

Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing

et al. 2012

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Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis of the results identified 25 distinct C. felis MLVA patterns. In parallel, a recently described multilocus sequence typing scheme for the typing of Chlamydia was applied to 13 clinical samples with 12 different C. felis MLVA patterns. Rare sequence differences were observed. Thus, the newly developed MLVA system provides a highly sensitive high-resolution test for the differentiation of C. felis isolates from different origins that is suitable for molecular epidemiological studies.

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Conjunctivitis Due to Chlamydophila felis (Chlamydia psittaci Feline Pneumonitis Agent) Acquired From a Cat: Case Report with Molecular Characterization of Isolates from the Patient and Cat

Cited by 29 publications

References 20 publications

Chlamydophila felisCF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis ofC. felisInfection

Chlamydophila felisCF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis ofC. felisInfection

Clinical and Microbiological Diagnosis of Trachoma in Children Living in Rural Areas in the District of Attock, Punjab, Pakistan

Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing

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