Background: Dengue is under-reported in the indicators and directories of health statistics in Togo and the diagnostic hypothesis of dengue during medical consultations is often not mentioned. Objective: The objective is to assess healthcare workers' knowledge of dengue and its management in public health centers in the health district of Lomé Commune. Materials and Methods: This is a cross-sectional and descriptive study that took place from November 1, 2019 to February 29, 2020 in the health district of Lomé Commune. Results: This study involved 280 healthcare workers out of 310 concerned, i.e., a participation rate of 90.32%, distributed over the 22 public health