When assessing the sustainability of novel technologies and materials systems, the question arises as to how a robust sustainability assessment for technology and material developments with a low readiness level is nevertheless possible and how the necessary data can be collected for this purpose. Based on this research question, TAPAS as a new, tiered methodological framework for a prospective assessment of the sustainability aspects of novel technologies and materials systems is presented.Within the conceptual development of TAPAS, important groundwork for the new methodological framework was devoted to the methodological interface between biomimetics and sustainability research. We selected the plant growth form liana as an example; biological concepts were used to sharpen the sustainability strategies of efficiency, consistency, and sufficiency and to derive practical design principles for more sustainable products in the technosphere.On this basis, we developed guiding principles for TAPAS and defined a total of five stages of analysis, allowing self-reflection to be integrated into the research and innovation process. In some cases, established instruments were adopted or refined, such as those used in the prospective screening of chemicals and in life cycle assessment studies. In addition, new methodological ground has been broken to meet the demand for an integrated and normatively based assessment that covers both benefit and risk aspects. This applies in particular to the domain of an in-depth benefit analysis and, for the first time, a set of 30 indicators has been developed that establish a direct link to the 2030 Agenda and its Sustainable Development Goals.We conducted two case studies to test the practical applicability of TAPAS. They showed that the developed tools are in principle suitable for basic research projects. Concrete recommendations for more sustainable design options (e.g., substitution of toxicologically problematic solvents) were provided.