Connective tissue growth factor (CTGF) and cancer progression

Chu, Chia-Yu; Chang, Cheng-Chi; Prakash, Ekambaranellore; Kuo, Min-Liang

doi:10.1007/s11373-008-9264-9

Cited by 124 publications

(114 citation statements)

References 109 publications

(113 reference statements)

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“…These included regulators of TGF-␤/Nodal and Wnt signaling pathways, Lefty A/B, Cerberus, and sFRP1/2. Other identified factors, such as hepatoma-derived growth factor and CTGF, are of particular interest because of their relevance in the biology of cancer cells (30,31). Interestingly, after pathway analysis of the filtered datasets, we observed a significant enrichment for molecules involved in cell motility, apoptosis, and invasion of cancer cells (Fig.…”

Section: Stem Cell Factors Identified In Conditioned Matrigelmentioning

confidence: 88%

Mass Spectrometry–based Proteomic Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture

Hughes

Radan

Chang

et al. 2012

Molecular & Cellular Proteomics

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The cellular microenvironment comprises soluble factors, support cells, and components of the extracellular matrix (ECM) that combine to regulate cellular behavior. Pluripotent stem cells utilize interactions between support cells and soluble factors in the microenvironment to assist in the maintenance of self-renewal and the process of differentiation. However, the ECM also plays a significant role in shaping the behavior of human pluripotent stem cells, including embryonic stem cells (hESCs) and induced pluripotent stem cells. Moreover, it has recently been observed that deposited factors in a hESC-conditioned matrix have the potential to contribute to the reprogramming of metastatic melanoma cells. Therefore, the ECM component of the pluripotent stem cell microenvironment necessitates further analysis.In this study we first compared the self-renewal and differentiation properties of hESCs grown on Matrigel™ pre-conditioned by hESCs to those on unconditioned Matrigel™. We determined that culture on conditioned Matrigel™ prevents differentiation when supportive growth factors are removed from the culture medium. To investigate and identify factors potentially responsible for this beneficial effect, we performed a defined SILAC MSbased proteomics screen of hESC-conditioned Matrigel™. From this proteomics screen, we identified over 80 extracellular proteins in matrix conditioned by hESCs and induced pluripotent stem cells. These included matrixassociated factors that participate in key stem cell pluripotency regulatory pathways, such as Nodal/Activin and canonical Wnt signaling. This work represents the first investigation of stem-cell-derived matrices from human pluripotent stem cells using a defined SILAC MS-based proteomics approach. Molecular & Cellular Proteomics

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Section: Stem Cell Factors Identified In Conditioned Matrigelmentioning

confidence: 88%

Mass Spectrometry–based Proteomic Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture

Hughes

Radan

Chang

et al. 2012

Molecular & Cellular Proteomics

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“…Associations have been reported between an increase in CCN2 and better survival rate in patients with esophageal squamous cell carcinoma and chondrosarcoma (22) as well as with decreased metastasis in a colon cancer mouse model (23). In contrast, CCN2 mediates functions such as proliferation, migration, differentiation, and survival, leading to enhanced angiogenesis in endothelial cells (24). The role of CCN2 in different types of cancer is believed to vary considerably, depending on the type of tissue involved.…”

Section: Introductionmentioning

confidence: 99%

Glutathione S-Transferase Mu2 Suppresses Cancer Cell Metastasis in Non–Small Cell Lung Cancer

Tang

Lai

et al. 2013

Molecular Cancer Research

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Glutathione S-transferase mu2 (GST-M2) is a phase II detoxification enzyme. Low expression of GST-M2 in lung cancers is due to hypermethylation of its promoter. Lung cancer with the GST mu-null genotype is associated with shorter survival. However, a correlation between GST-M2 and important clinical parameters, as well as the migration of GST-M2-defective cells in lung cancer, has not been established. In the present study, we investigate the role of GST-M2 in cell migration and actin disassembly in lung cancer cells. GST-M2 and CCN2 mRNA levels were significantly reduced in non-small cell lung cancer (NSCLC) tumors when compared with matched normal lung tissues in 82 patients with NSCLC. We found that high expressions of both GST-M2 and CCN2 are correlated with favorable survival of patients with lung cancer when compared with similar patients without GST-M2 or CCN2 expression. GST-M2 can induce CCN2 expression by driving the CCN2 proximal promoter. Overexpression of GST-M2 decreases the formation of filopodia, resulting in remodeling of the reorganized cytoskeletons. Overexpression of GST-M2 significantly suppressed cancer cell migration on wound-healing assay. In addition, overexpression of GST-M2 dramatically reduced tumor growth and metastasis in a xenograft mouse model. These data highlight the potential of GST-M2 as a novel tumor suppressor. GST-M2 increases the expression of CCN2 in lung cancer cells, which inhibits cancer cell migration in lung cancer and animal models. Mol Cancer Res; 11(5); 518-29. Ó2013 AACR.

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“…Aberrant CTGF expression has been documented in numerous types of cancer to date (Chu, Chang et al 2008;Wells, Howlett et al 2014) including 75% of pre-B ALL (Boag, Beesley et al 2007 ). The modular, pleiotropic nature of CTGF means that fully characterising its expression is of critical importance, as novel isoforms may exist that contribute to, or promote, the leukaemic phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Aberrant CTGF expression has been identified in numerous other neoplasms (Chu, Chang et al 2008;Wells, Howlett et al 2014), suggesting a relationship between dysregulated CTGF expression and cancer more generally. Associations between CTGF mRNA expression and poor patient outcome in both adult and childhood ALL suggest that CTGF may have an important role in leukemia biology (Sala-Torra, Gundacker et al 2007;Kang, Chen et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Novel CT domain-encoding splice forms of CTGF/CCN2 are expressed in B-lineage acute lymphoblastic leukaemia

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Connective tissue growth factor (CTGF) and cancer progression

Cited by 124 publications

References 109 publications

Mass Spectrometry–based Proteomic Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture

Mass Spectrometry–based Proteomic Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture

Glutathione S-Transferase Mu2 Suppresses Cancer Cell Metastasis in Non–Small Cell Lung Cancer

Novel CT domain-encoding splice forms of CTGF/CCN2 are expressed in B-lineage acute lymphoblastic leukaemia

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