Connective tissue sheath of hair follicle is a major source of dermal type I procollagen in human scalp

Oh, Jang‐Hee; Kwon, Oh Sang; Kim, Mi Hyang; Jo, Seong Jin; Han, Ji Hoon; Kim, Kyu Han; Eun, Hee Chul; Chung, Jin Ho

doi:10.1016/j.jdermsci.2012.09.006

Cited by 4 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extracellular material visible in Figure 8(b) is most probably mainly collagen, which is the most abundant protein in the ECM of the human body and which is known to be produced in high amounts by NBDS cells. 12 Collagen-or, more precisely, collagen type I-is also the major constituent of natural bone. The collagen matrix in the bones is filled with mineral particles, in particular hydroxyapatite and other calcium compounds.…”

Section: Discussionmentioning

confidence: 99%

“…11 Notably, it has been shown that NBDS cells produce up to five times more collagen type I than other skin-derived fibroblasts. 12 This is of particular interest in bone formation by means of tissue engineering techniques. NBDS cells express all important cell markers of mesenchymal stem cells and can therefore be expected to differentiate into other cell types within the family of mesenchymal cells of the connective tissue, which includes both fibroblasts and bone cells.…”

Section: Introductionmentioning

confidence: 99%

“…Although NBDS cells cannot form new hair follicles (unlike other cell types in the follicle), they possess stem–cell character . Notably, it has been shown that NBDS cells produce up to five times more collagen type I than other skin‐derived fibroblasts . This is of particular interest in bone formation by means of tissue engineering techniques.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization

Heitz

Plamadeala

Wiesbauer

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

The main aim of this work was to stimulate bone‐forming cells to produce three‐dimensional networks of mineralized proteins such as those occurring in bones. This was achieved by a novel approach using a specific type of mesenchymal progenitor cells (i.e., primary fibroblast cells from human hair roots) seeded on to polymer scaffolds. We wrote polymer microstructures with one or more levels of quadratic pores on to a flexible substrate by means of two‐photon polymerization using a Ti‐sapphire femtosecond laser focused into a liquid acrylate‐based resin containing a photoinitiator. Progenitor cells, differentiated into an osteogenic lineage by the use of medium supplemented with biochemical stimuli, can be seeded on to the hydrophilic three‐dimensional scaffolds. Due to confinement to the microstructures and/or mechanical interaction with the scaffold, the cells are stimulated to produce high amounts of calcium‐binding proteins, such as collagen type I, and show an increased activation of the actin cytoskeleton. The best results were obtained for quadratic pore sizes of 35 µm: the pore volumes become almost filled with both cells in close contact with the walls of the structure and with extracellular matrix material produced by the cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 891–899, 2017.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization

Heitz

Plamadeala

Wiesbauer

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…In human hair follicle, connective tissue sheath (CTS) and dermal papilla cells are the main sources of collagen type I. Their production of collagen type I increases during anagen phase of hair cycle and application of trans‐retinoic acid . This collagen type I plays a crucial role in maintaining the structure of hair follicle CTS and DP as well as contributing to dermal matrix of human skin.…”

Section: Resultsmentioning

confidence: 99%

Quantitative proteomic analysis of dermal papilla from male androgenetic alopecia comparing before and after treatment with low‐level laser therapy

2019

View full text Add to dashboard Cite

Background Currently, low‐level laser therapy (LLLT) has been approved as a new treatment for androgenetic alopecia (AGA). However, it has not been elucidated how LLLT promotes hair growth in vivo. Objectives To investigate the change in protein expression from dermal papilla (DP) tissues in male AGA patients after LLLT treatment using liquid chromatography tandem mass spectrometry (LC‐MS/MS) analysis. Methods This is an open‐label, prospective, single‐arm study obtained punch scalp biopsy specimens from patients with AGA before and after LLLT treatment. Each subject was self‐treated with helmet type of LLLT (655 nm, 5 mW) device at home for 25 minutes per treatment every other day for 24 weeks. LC‐MS/MS analysis based on the dimethyl labeling strategy for protein quantification was used to identify proteins expressed in DP tissues from AGA patients. Results Proteomic analysis revealed 11 statistically significant up‐regulated and 2 down‐regulated proteins in LLLT treated DP compared with baseline (P < 0.05). A bioinformatic analysis signifies that these proteins are involved in several biological processes such as regulation of cellular transcription, protein biosynthesis, cell energy, lipid homeostasis, extracellular matrix (ECM), ECM structural constituent, cell‐cell/cell‐matrix adhesion as well as angiogenesis. ATP‐binding cassette sub‐family G member, a transporter involved in cellular lipid homeostasis, was the most up‐regulated protein. Additionally, LLLT increased the main ECM proteins in DP which results in a bigger volume of DP and a clinical improvement of hair diameter in AGA patients. Conclusion We identified the proteome set of DP proteins of male patients with AGA treated with LLLT which implicates the role of LLLT in promoting hair growth and reversing of miniaturization process of AGA by enhancing DP cell function. Our results strongly support the benefit of LLLT in the treatment of AGA. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

show abstract

“…To this end, we developed a cell-therapeutic approach based on the intradermal injection of autologous, cultured mesenchymal cells, isolated from the non-bulbar dermal sheath (NBDS) of hair follicles. The NBDS represents a unique source of fibroblasts, which express several proteins that potentially support ECM maintenance, most notably type 1 collagen [6][7][8]. Cultured NBDS cells provide a promising platform to treat intrinsically or extrinsically aged/damaged skin such as fine wrinkles by implanting UV naïve, collagen-producing NBDS cells directly to the affected area.…”

Section: Introductionmentioning

confidence: 99%

Autologous Cell Therapy for Aged Human Skin: A Randomized, Placebo-Controlled, Phase-I Study

Marini

Jaenicke

Goessens-Rück³

et al. 2019

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Introduction: Skin ageing involves senescent fibroblast accumulation, disturbance in extracellular matrix (ECM) homeostasis, and decreased collagen synthesis. Objective: to assess a cell therapy product for aged skin (RCS-01) consisting of ~25 x 10 6 cultured, autologous cells derived from anagen hair follicle non-bulbar dermal sheath (NBDS). Methods: For each subject in the verum group, four areas of buttock skin were injected intradermally 1 or 3 times at monthly intervals with RCS-01, cryomedium, or needle penetration without injection; in the placebo group RCS-01 was replaced by cryomedium. The primary endpoint was assessment of local adverse event profiles. As secondary endpoints, expression of genes related to ECM homeostasis was assessed in biopsies from randomly selected volunteers in the RCS-01 group taken 4 weeks after the last injection. Results: Injections were well tolerated with no severe adverse events reported 1 year after first injection. When compared with placebo treated skin, a single treatment with RCS-01 resulted in a significant upregulation of TGFß1, CTGF, COL1A1, COL1A2, COL3A1, and lumican mRNA expression. Limitations: The cohort size was insufficient for dose ranging evaluation and subgroup analyses of efficacy. Conclusions: RCS-01 therapy is well tolerated and associated with a gene expression response consistent with an improvement of ECM homeostasis.

show abstract

Connective tissue sheath of hair follicle is a major source of dermal type I procollagen in human scalp

Cited by 4 publications

References 10 publications

Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization

Bone‐forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two‐photon polymerization

Quantitative proteomic analysis of dermal papilla from male androgenetic alopecia comparing before and after treatment with low‐level laser therapy

Autologous Cell Therapy for Aged Human Skin: A Randomized, Placebo-Controlled, Phase-I Study

Contact Info

Product

Resources

About