Connexin 43 trafficking and regulation of gap junctional intercellular communication alters ovarian cancer cell migration and tumorigenesis

Mulchandani, Vaishali; Banerjee, Anurag; Vadlamannati, Arunima Vijaya; Kumar, Saurav; Sarma, Jayasri Das

doi:10.1016/j.biopha.2023.114296

Cited by 7 publications

(4 citation statements)

References 55 publications

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“…CX43 is a type of intercellular communication protein that participates in the process of intercellular communication and coordination (14). During the maturation process of oocytes, CX43 can regulate the electrophysiological activity of oocytes, promote intercellular communication and synchronization, and contribute to the normal development and maturation of oocytes (15).…”

Section: Discussionmentioning

confidence: 99%

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Yang

Duan

et al. 2023

Preprint

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Background Brilliant cresyl blue (BCB) staining can stain oocytes and differentiated oocytes will lead to different developmental outcomes. This technique has been studied in multiple species, but it is still unclear whether buffalo oocytes can be used for developmental potential prediction through BCB staining methods. This study used the BCB staining method to group buffalo oocytes (BCB + and BCB-) and perform in vitro maturation, in vitro fertilization, and embryo culture. By statistical analysis, the effect of BCB staining on predicting the developmental potential of buffalo oocytes will be explored. At the same time, molecular biology techniques will be used to detect gap junction protein and oxidative stress-related indicators to explore the molecular mechanism of BCB staining predicting oocyte cell developmental potential. Methods The oocytes were divided into BCB + and BCB- groups using BCB staining technique. And then mainly uses in vitro maturation, in vitro fertilization and embryo culture techniques of buffalo oocytes to analyze their developmental potential, and uses immunofluorescence staining to detect the expression level of CX43 protein, DCFH-DA probe staining to detect ROS levels, and qPCR to detect the expression levels of the antioxidant related genes SOD2 and GPX1. Results Our results showed that the in vitro maturation rate, embryo cleavage rate, and blastocyst rate of buffalo oocytes in the BCB + group were significantly higher than those in the BCB- group and the control group (P < 0.05). The expression level of CX43 protein in the BCB + group was higher than that in the BCB- group both before and after maturation (P < 0.05). The intensity of ROS in the BCB + group was significantly lower than that in the BCB- group (P < 0.05), and the expression levels of the antioxidant-related genes SOD2 and GPX1 in the BCB + group were significantly higher than those in the BCB- group (P < 0.05). Conclusions BCB staining can effectively predict the developmental potential of buffalo oocytes. The results of BCB staining are positively correlated with the expression of gap junction protein and antioxidant-related genes and negatively correlated with the ROS level, suggesting that the mechanism of BCB staining in predicting the developmental potential of buffalo oocytes may be closely related to antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Yang

Duan

et al. 2023

Preprint

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“…Cx43 appears to play a role in TNT formation and their morphological properties. As an example, GJIC-deficient ovarian cancer cells developed shorter TNTs than their GJIC-competent controls ( 172 ). In keeping with these observations, the deletion, knockdown, or inhibition of Cx43 has been shown to significantly reduce the length and abundance of TNTs ( 173 , 174 , 175 , 176 ).…”

Section: Noncanonical Roles Of Connexins and Connexins Fragmentsmentioning

confidence: 99%

Diversity in connexin biology

Lucaciu,

Leighton,

Hauser

et al. 2023

Journal of Biological Chemistry

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“…On the other hand, in vitro studies found that these highly tumorigenic ID8-VEGF cells had reduced gap junctional intracellular communications compared to ID8 cells. Overall, the researchers utilised the syngeneic mice model to explain the reduction in gap junctions mediated intracellular communication in the tumorigenic ovarian cancer cell lines [79].…”

Section: Syngeneic Mouse Modelsmentioning

confidence: 99%

Mice as an Experimental Model to Understand the Pathobiology of Diseases

Jana¹,

Karmakar²,

Hazra³

et al. 2023

Rodents and Their Role in Ecology, Medicine and Agriculture

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Murine models are widely used in scientific research because they share many genetic similarities with humans, making them a valuable tool for studying various diseases. C57BL/6 is an experimental mouse model to study the demyelination and inflammation etiology of Multiple Sclerosis (MS). Intracranial inoculation of neurotropic murine β-coronavirus strain of Mouse hepatitis virus in C57BL/6 mice induces demyelination with or without axonal loss, providing many insights regarding the mechanism of MS as well as SARS-CoV-2 mediated pulmonary and neuro pathology in humans. By selectively using knockout mice in the wild-type C57BL/6 background, researchers can gain insights into the immunomodulatory nexus and can identify pathways involved in immune regulation which further can be efficiently studied with CD4-/-, CD40-/- and CD40L-/- mice. In addition, C57BL/6 mice can also be used to generate syngeneic mouse models to investigate the etiology and mechanism of various cancers, including ovarian cancer. Similarly, along with C57BL/6 mice, different immunocompromised mice models, such as nude mice, SCID mice, and NOD/SCID mice, can be used to study the etiology, host-tumour interaction, function of the microenvironment, and tumour heterogeneity in tumour metastasis.

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Connexin 43 trafficking and regulation of gap junctional intercellular communication alters ovarian cancer cell migration and tumorigenesis

Cited by 7 publications

References 55 publications

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Diversity in connexin biology

Mice as an Experimental Model to Understand the Pathobiology of Diseases

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