2019
DOI: 10.1038/s42003-019-0590-4
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Consensus model of a cyanobacterial light-dependent protochlorophyllide oxidoreductase in its pigment-free apo-form and photoactive ternary complex

Abstract: Photosynthetic organisms employ two different enzymes for the reduction of the C17 = C18 double bond of protochlorophyllide (Pchlide), yielding the chlorophyll precursor chlorophyllide. First, a nitrogenase-like, light-independent (dark-operative) Pchlide oxidoreductase and secondly, a light-dependent Pchlide oxidoreductase (LPOR). For the latter enzyme, despite decades of research, no structural information is available. Here, we use protein structure modelling, molecular dynamics (MD) simulations combined wi… Show more

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Cited by 11 publications
(16 citation statements)
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“…To rule out that the flexible N-terminal His-tag contributes to the obtained ab initio models, we also included an El LPOR sample, which possessed an eight-amino acid-long His-tag at the C-terminus instead of the N-terminus ( El LPOR-cHis). The corresponding molecular masses estimated from the SAXS data ( table 2 ) are in good agreement with the theoretical molecular masses, indicating that all studied apo LPORs are monomeric, as also previously shown for Te LPOR ( Schneidewind et al. 2019 ).…”
Section: Resultssupporting
confidence: 86%
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“…To rule out that the flexible N-terminal His-tag contributes to the obtained ab initio models, we also included an El LPOR sample, which possessed an eight-amino acid-long His-tag at the C-terminus instead of the N-terminus ( El LPOR-cHis). The corresponding molecular masses estimated from the SAXS data ( table 2 ) are in good agreement with the theoretical molecular masses, indicating that all studied apo LPORs are monomeric, as also previously shown for Te LPOR ( Schneidewind et al. 2019 ).…”
Section: Resultssupporting
confidence: 86%
“…4 , lower panels; see Materials and Methods for details). As previously shown for Te LPOR ( Schneidewind et al. 2019 ), the overall shape of the molecule resembles a bowling-pin appearing to consist of a larger and smaller subdomain ( fig.…”
Section: Resultssupporting
confidence: 75%
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“…LPOR is encoded by one or several nuclear genes ( POR genes) and uses NADPH as an electron donor in the presence of light for the reduction of the C17-C18 double bond (reviewed in [ 65 ]). The holocomplex is proposed to be dimeric [ 66 ]. The DPOR protein is a complex formed by the L, N and B subunits encoded in the chloroplast genome by the chlL , chlN and chlB genes.…”
Section: Fe-s Protein-dependent Metabolic Processes In Algal Chlormentioning
confidence: 99%
“…A crystal structure of the ternary complex is not yet available, which has been attributed to the oligomerisation of POR when Pchlide is bound. Oligomerisation has also been observed by mass spectrometry and small‐angle X‐ray scattering [28,29].…”
Section: Introductionmentioning
confidence: 99%