Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Loureiro, B.; Block, J.; Favoreto, M. G.; Carambula, Silvia; Pennington, Kathleen A.; Ealy, Alan D.; Hansen, Peter J.

doi:10.1530/rep-10-0511

Cited by 42 publications

(30 citation statements)

References 22 publications

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“…For the D18 pregnant heifers, mean antiviral activity was 180 000 IU/flush. The obtained antiviral activities appear to be lower than reported earlier, because Short et al [56] found 10 5 IU/flush on D15 and 10 7 IU/flush on D17 and Loureiro et al [57] found up to 6 3 10 6 IU/flush on D15. Altogether, the observed antiviral activity in uterine flushings after the 2 days of IFNA2 administration were in a physiological range comparable to antiviral activity in the uterine lumen during early pregnancy due to production of IFNT by the elongating conceptus.…”

Section: Discussioncontrasting

confidence: 55%

Comparison of the Effects of Early Pregnancy with Human Interferon, Alpha 2 (IFNA2), on Gene Expression in Bovine Endometrium1

Bauersachs¹,

Ulbrich

Reichenbach

et al. 2012

Biology of Reproduction

109

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Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. To elucidate specific effects of bovine IFNT and of other conceptus-derived factors, endometrial gene expression changes during early pregnancy were compared to gene expression changes after intrauterine application of human IFNA2. In experiment 1, endometrial tissue samples were obtained on Day (D) 12, D15, and D18 postmating from nonpregnant or pregnant heifers. In experiment 2, heifers were treated from D14 to D16 of the estrous cycle with an intrauterine device releasing IFNA2 or, as controls, placebo lipid extrudates or PBS only. Endometrial biopsies were performed after flushing the uterus. All samples from both experiments were analyzed with an Affymetrix Bovine Genome Array. Experiment 1 revealed differential gene expression between pregnant and nonpregnant endometria on D15 and D18. In experiment 2, IFNA2 treatment resulted in differential gene expression in the bovine endometrium. Comparison of the data sets from both studies identified genes that were differentially expressed in response to IFNA2 but not in response to pregnancy on D15 or D18. In addition, genes were found that were differentially expressed during pregnancy but not after IFNA2 treatment. In experiment 3, spatiotemporal alterations in expression of selected genes were determined in uteri from nonpregnant and early pregnant heifers using in situ hybridization. The overall findings of this study suggest differential effects of bovine IFNT compared to human IFNA2 and that some pregnancy-specific changes in the endometrium are elicited by conceptus-derived factors other than IFNT.endometrium, hormone action, pregnancy, reproductive immunology, uterus

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Section: Discussioncontrasting

confidence: 55%

Comparison of the Effects of Early Pregnancy with Human Interferon, Alpha 2 (IFNA2), on Gene Expression in Bovine Endometrium1

Bauersachs¹,

Ulbrich

Reichenbach

et al. 2012

Biology of Reproduction

109

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“…Several uterine- and conceptus-derived factors such as fibroblast growth factors (e.g. FGF2, 10) and colony stimulating factor 2 stimulate IFNT production from bovine trophectoderm [16,18,31,32]. These and other factors may play a part in the rapid increase in IFNT production during elongation.…”

Section: Resultsmentioning

confidence: 99%

The expression and potential function of bone morphogenetic proteins 2 and 4 in bovine trophectoderm

Pennington

Ealy

2012

Reprod Biol Endocrinol

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BackgroundBone morphogenetic proteins (BMPs) were first described for their roles in bone formation, but they now also are known to possess additional activities, including those relating to embryogenesis. The objectives of this work were to 1) determine if peri-attachment bovine conceptuses and bovine trophoblast cells (CT1) contain transcripts for BMP2 and 4, an innate inhibitor noggin (NOG), and BMP2/4 receptors (BMPRII, ACVR1, BMPR1A, BMPR1B), and 2) determine if BMP2 or 4 supplementation to CT1 cells affects cell proliferation, differentiation or trophoblast-specific gene expression.MethodsRNA was isolated from day 17 bovine conceptuses and CT1 cells. After RT-PCR, amplified products were cloned and sequenced. In other studies CT1 cells were treated with BMP2 or 4 at various concentrations and effects on cell viability, cell differentiation and abundance of IFNT and CSH1 mRNA were evaluated.ResultsTranscripts for BMP2 and 4 were detected in bovine conceptuses and CT1 cells. Also, transcripts for each BMP receptor were detected in conceptuses and CT1 cells. Transcripts for NOG were detected in conceptuses but not CT1 cells. Cell proliferation was reduced by BMP4 but not BMP2 supplementation. Both factors reduced IFNT mRNA abundance but had no effect on CSH1 mRNA abundance in CT1 cells.ConclusionsThe BMP2/4 ligand and receptor system presides within bovine trophectoderm prior to uterine attachment. BMP4 negatively impacts CT1 cell growth and both BMPs affect IFNT mRNA abundance.

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“…In cattle and sheep, endometrial derived proteins, e.g. IGFBP1 ), GRP (Song et al 2008), LGALS15 (Lewis et al 2007), CSF2 (Loureiro et al 2011), actively enhance migration, proliferation and/or attachment of the conceptus trophectoderm. Therefore, inhibitors of protein and peptide cleavage in the ULF would be important to ensure that full-length functional forms of at least some of these proteins, when transported from the endometrium into the uterine lumen, are available for use by the elongating conceptus.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle

Forde¹,

McGettigan²,

Mehta³

et al. 2014

Reproduction

117

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The aims of this study were i) to characterize the global changes in the composition of the uterine luminal fluid (ULF) from pregnant heifers during pregnancy recognition (day 16) using nano-LC MS/MS; ii) to describe quantitative changes in selected proteins in the ULF from days 10, 13, 16 and 19 by Isobaric tags for Relative and Absolute Quantification (iTRAQ) analysis; and iii) to determine whether these proteins are of endometrial or conceptus origin, by examining the expression profiles of the associated transcripts by RNA sequencing. On day 16, 1652 peptides were identified in the ULF by nano-LC MS/MS. Of the most abundant proteins present, iTRAQ analysis revealed that RPB4, TIMP2 and GC had the same expression pattern as IFNT, while the abundance of IDH1, CST6 and GDI2 decreased on either day 16 or 19. ALDOA, CO3, GSN, HSP90A1, SERPINA31 and VCN proteins decreased on day 13 compared with day 10 but subsequently increased on day 16 (P!0.05). Purine nucleoside phosphorylase (PNP) and HSPA8 decreased on day 13, increased on day 16 and decreased and increased on day 19 (P!0.05). The abundance of CATD, CO3, CST6, GDA, GELS, IDHC, PNPH and TIMP2 mRNAs was greater (P!0.001) in the endometrium than in the conceptus. By contrast, the abundance of ACTB, ALDOA, ALDR, CAP1, CATB, CATG, GD1B, HSP7C, HSP90A, RET4 and TERA was greater (P!0.05) in the conceptus than in the endometrium. In conclusion, significant changes in the protein content of the ULF occur during the pre-implantation period of pregnancy reflecting the morphological changes that occur in the conceptus.

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Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression

Cited by 42 publications

References 22 publications

Comparison of the Effects of Early Pregnancy with Human Interferon, Alpha 2 (IFNA2), on Gene Expression in Bovine Endometrium1

Comparison of the Effects of Early Pregnancy with Human Interferon, Alpha 2 (IFNA2), on Gene Expression in Bovine Endometrium1

The expression and potential function of bone morphogenetic proteins 2 and 4 in bovine trophectoderm

Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle

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