2018
DOI: 10.1186/s12864-018-4479-2
|View full text |Cite
|
Sign up to set email alerts
|

Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice

Abstract: BackgroundMaternal separation models in rodents are widely used to establish molecular mechanisms underlying prolonged effects of early life adversity on neurobiological and behavioral outcomes in adulthood. However, global epigenetic signatures following early life stress in these models remain unclear.ResultsIn this study, we carried out a ChIP-seq analysis of H3K4 trimethylation profile in the prefrontal cortex of adult male mice with a history of early life stress. Two types of stress were used: prolonged … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
7
1
5

Year Published

2018
2018
2024
2024

Publication Types

Select...
7
1
1

Relationship

2
7

Authors

Journals

citations
Cited by 22 publications
(17 citation statements)
references
References 83 publications
4
7
1
5
Order By: Relevance
“…To determine if epigenetic changes in H3K4me3 marks were associated with transcriptional activation we first improved the sheep genome annotation of transcripts and transcription start sites (TSSs) with PT RNA and a combination of Cap Analysis of Gene Expression (CAGE-seq, Supplementary Data 4) 32 and ISOSEQ long-read RNA-seq (SRA: PRJNA391103, see methods for details). Using this improved annotation we identified H3K4me3 peaks and found the distribution on genomic features to be similar to previous studies [33][34][35] , validating our approach (see methods section).…”
Section: Resultssupporting
confidence: 81%
“…To determine if epigenetic changes in H3K4me3 marks were associated with transcriptional activation we first improved the sheep genome annotation of transcripts and transcription start sites (TSSs) with PT RNA and a combination of Cap Analysis of Gene Expression (CAGE-seq, Supplementary Data 4) 32 and ISOSEQ long-read RNA-seq (SRA: PRJNA391103, see methods for details). Using this improved annotation we identified H3K4me3 peaks and found the distribution on genomic features to be similar to previous studies [33][34][35] , validating our approach (see methods section).…”
Section: Resultssupporting
confidence: 81%
“…To determine if epigenetic changes in H3K4me3 marks were associated with transcriptional activation we first improved the sheep genome annotation of transcripts and transcription start sites (TSS) with PT RNA and a combination of Cap Analysis of Gene Expression (CAGE-seq, Supplementary Data 4) 32 and ISOSEQ long-read RNA-seq (SRA: PRJNA391103, see methods for details). Using this improved annotation we identified H3K4me3 peaks and found the distribution on genomic features to be similar to previous studies [33][34][35] , validating our approach (see methods section). Next we identified seasonally expressed genes, as defined by RNA-seq analysis of differentially regulated genes (DEGs) in the SP to LP and LP to SP transfers (Supplementary Data 3), and observed a strong correlation between seasonal gene expression and H3K4me3 peaks around the transcription start sites (TSS's)( Fig.…”
Section: Epigenetic Regulation Of the Seasonal Transcriptomesupporting
confidence: 81%
“…We clustered SP and LP time-series profiles using Partitioning Around Medoids (PAM) ( Fig. 4g) peaks were closely resembling to the previous reports 33,34 ( Supplementary Fig. 6a).…”
Section: Diurnal Comparisonsupporting
confidence: 77%
“…DNA methylation in the NR1D1 promoter is greater in humans with a history of child abuse [55]. In our study of delayed effects of early-life stress on the genomic landscape of H3K4me3 in adult male mice [56], there was a significant increase in the amount of active-chromatin modification H3K4me3 in the promoter region of Nr1d1. These data are suggestive of a more active promoter of this gene in the animals with a history of MS.…”
Section: Early-life Stress Increases the Expression Of Nr1d1 But Doesmentioning
confidence: 58%