The triptych icons are unique icons called traveling icons. Several microscopes were used to examine and study the layers of the icon such as stereo, digital and SEM microscopes. Different analytical methods such as XRD, Raman, FTIR and SEM-EDX were complementary to each other and helped in identifying the different components of the icon. The use of shellac as a varnish, calcium carbonate as a preparation layer, barium chromate, Prussian blue, titanium white and cadmium yellow as pigments are strong results that are not commonly used in icons. Spectroscopic studies dated the icon to the end of the 19 th century and the beginning of the twentieth century.