Conservation in sequence and affinity of human and rodent PDGF ligands and receptors

Herren, Barbara; Weyer, Karl Aloys; Rouge, Marianne; Lötscher, Pius; Pech, Michael

doi:10.1016/0167-4781(93)90127-y

Cited by 28 publications

(18 citation statements)

References 38 publications

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“…In primary cultured rat brain and retinal pericytes used as positive controls, these mRNAs were amplified by each specific primer at the same size with TR-rPCTs cells. Moreover, the sequences of specific PCR products, which were amplified in TR-rPCTs cells, were 100% homologous with each gene (Kita et al, 1992;Singh et al, 1992;Herren et al, 1993;Mandriota and Pepper, 1998). On the other hand, vWF as an endothelial cell marker was not detected in TR-rPCTs cells and rat primary cultured retinal pericytes (Fig.…”

Section: Characterization Of the Immortalized Rat Retinal Pericyte Cementioning

confidence: 98%

Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)

Kondo

Hosoya

Hori

et al. 2003

Cell Struct. Funct.

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ABSTRACT. The purpose of this study was to establish and characterize a retinal pericyte cell line from retinal capillaries of transgenic rats harboring the temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), and to apply this to the co-culture with a retinal capillary endothelial cell line. The conditionally immortalized rat retinal pericyte cell lines (TR-rPCTs), which express a temperature-sensitive large T-antigen, were obtained from two tsA58 Tg rats. These cell lines had a multicellular nodule morphology and reacted positively with von Kossa staining, a marker of calcification. TR-rPCTs cells expressed mRNA of pericyte markers such as rat intercellular adhesion molecule-1, platelet-derived growth factor-receptor β, angiopoietin-1, and osteopontin. Western blot analysis indicated that α-smooth muscle actin (α-SMA) was expressed in TR-rPCT3 and 4 cells. In contrast, α-SMA was induced by transforming growth factor-β1 and its enhancement was reduced by basic fibroblast growth factor in TR-rPCT1 and 2 cells. When TR-rPCT1 cells were cultured with a rat retinal endothelial cell line (TR-iBRB2) in a contact co-culture system, the number of TR-iBRB2 cells were significantly reduced in comparison with that of a single culture of TR-iBRB2 cells, suggesting that physical contact between pericytes and retinal endothelial cells is important for the growth of retinal endothelial cells. In conclusion, conditionally immortalized retinal pericyte cell lines were established from tsA58 Tg rats. These cell lines exhibited the properties of retinal pericytes and can be applied in co-culture systems with a retinal capillary endothelial cell line.Key words: retinal pericyte cell line/SV 40 large T-antigen/co-culture/retinal endothelial cell growth/α-smooth muscle actinRetinal pericytes, the cells that surround retinal capillary endothelial cells which make up the inner blood-retinal barrier (inner BRB), have been proposed to play a role in regulating endothelial cell proliferation (Orlidge and D 'Amore, 1987;Yamagishi et al., 1993). Platelet-derived growth factor-B (PDGF-B), which is one of the ligands of PDGFreceptor β (PDGF-Rβ), and PDGF-Rβ play an important role in microvessel assembly (Lindahl et al., 1997;Hellström et al., 1999). This suggests that PDGF-Rβ in pericytes and pericytes per se are important for forming the inner BRB.To investigate physiological roles of pericytes, primary *To whom correspondence should be addressed: Professor Tetsuya Terasaki, Ph.D., Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan. Tel: +81-22-217-6831, Fax: +81-22-217-6886 E-mail: terasaki@mail.pharm.tohoku.ac.jp Abbreviations: Ac-LDL, acetylated low density lipoprotein; Ang-1, angiopoietin-1; bFGF, basic fibroblast growth factor; BRB, blood-retinal barrier; BSA, bovine serum albumin; DMEM, Dulbecco's modified Eagle's medium; DiI, 1,[1][2][3]3,3',3'-tetramethyliodo-carbocyanine perchlorate; FBS, fetal bovine ...

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Section: Characterization Of the Immortalized Rat Retinal Pericyte Cementioning

confidence: 98%

Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)

Kondo

Hosoya

Hori

et al. 2003

Cell Struct. Funct.

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“…For synthesis of rat platelet-derived growth factor (PDGF) B chain, fibroblast growth factor-2 (FGF-2), connective tissue growth factor (CTGF) RNA probes, cDNA fragment was first generated by reverse transcription-PCR (RT-PCR) from the total RNA of rat mesangial cells or NRK-49F fibroblasts, using the following specific primer pairs: PDGF B chain, upstream 5'-TTGTGAGAAAGAAGC-CAGTC-3' (corresponding to bases 449 to 468) and downstream 5'-TGTGCTTAAACTTTCGGTGC-3' (corresponding to bases 642 to 661); FGF-2, upstream 5'-TCGGTCTCTCGGCTTCAGGC-3' (corresponding to bases 361 to 380) and downstream 5'-AGAGAGT-CAGCTCTTAGCAG-3' (corresponding to bases 984 to 1003); CTGF, upstream 5'-AGTCCTTCCAAAGCAGTTGC-3' (corresponding to bases 559 to 578) and downstream 5'-GCCTAAAATTGC-CAAGCCTG-3' (corresponding to bases 995 to 1014) (25)(26)(27). The products were subsequently subcloned into the pGEM-dT vectors (Promega, Madison, WI).…”

Section: Total Rna Extraction and Northern Blot Analysismentioning

confidence: 99%

Pentoxifylline Attenuated the Renal Disease Progression in Rats with Remnant Kidney

Lin¹,

Chen²,

Chien³

et al. 2002

Journal of the American Society of Nephrology

103

114

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Abstract. Previous studies have reported that pentoxifylline, a phosphodiesterase inhibitor, attenuates experimental mesangial proliferative glomerulonephritis. This study hypothesized that pentoxifylline could also attenuate the renal disease progression in rats with remnant kidney. After 5/6 subtotal nephrectomy, rats developed progressively elevated proteinuria and plasma creatinine, glomerulosclerosis, interstitial inflammation, and fibrosis, all of which were attenuated by 40 to 60% by pentoxifylline. However, the elevated BP was not changed by pentoxifylline. Pentoxifylline reduced the upregulation of monocyte chemoattractant protein-1 gene by 60% in the cortex of remnant kidney, as well as in a dose-dependent manner in the albumin-or angiotensin II-stimulated proximal tubular cells. It also reduced the upregulation of mitogenic and profibrogenic genes by 50%, including platelet-derived growth factor, fibroblast growth factor-2, transforming growth factor-␤ 1 , connective tissue growth factor, and types I and III collagen in the cortex of remnant kidney. Furthermore, pentoxifylline was found to decrease the numbers of interstitial myofibroblasts by 60% in the cortex of remnant kidney and suppress the proliferation of cultured interstitial fibroblasts. It also reduced the angiotensin II-induced or transforming growth factor-␤ 1

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“…17 An oligonucleotide 5 0 AAC GAT CAG AGT AGT GGT ATT TCA CC 3 0 complementary to the 28S ribosomal RNA (rRNA) (MWG Biotech, Ebersberg, Germany) was used to quantify Northern blots.…”

Section: Cdna Probesmentioning

confidence: 99%

Crosstalk between PDGF and IGF-I receptors in rat liver myofibroblasts: implication for liver fibrogenesis

Novosyadlyy

Dudás

Pannem

et al. 2006

Laboratory Investigation

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Insulin-like growth factor I (IGF-I) and platelet-derived growth factor (PDGF) have been identified as significant mitogens for liver myofibroblasts (LMFs), one of the cell populations playing a role in liver fibrogenesis. In the present work, we aimed to elucidate a possible interaction between PDGF receptor (PDGFR) and IGF-I receptor (IGF-IR) signaling in LMFs. Among different rat liver cells, PDGFR a-and b-subunits were mainly expressed in hepatic stellate cells and LMFs, and were upregulated during their in vitro cultivation. In LMFs, PDGF-BB (10 ng/ml) stimulated DNA synthesis approximately two-fold and this effect was similar to that of IGF-I. IGF-I and PDGF-BB differentially affected IGF-IR and PDGFR signaling. High concentrations of IGF-I decreased levels of IGF-IR and IRS-1 and inhibited the expression and activation of PDGFRa. PDGF-BB prevented IGF-I-induced downregulation of the IGF-IR, but did not affect expression of its cognate receptor subunits. Transphosphorylation of PDGFR and IGF-IR was not observed. PDGF effectively activated terminal MAP kinases, PI3 kinase and Akt kinase, whereas IGF-I demonstrated weaker effects. PLCc 1 was phosphorylated only in response to PDGF, but not to IGF-I. In rat LMFs, blockade of the IGF-IR via inhibition of the IGF-IR kinase completely abrogated IGF-and PDGF-induced mitogenesis and the ability of PDGF to phosphorylate PLCc 1 . In conclusion, the presented data demonstrate that the PDGFR signaling requires a functional IGF-IR and that PDGF-BB stabilizes the IGF-IR function through preventing the IGF-I-induced downregulation of the IGF-IR. These interactions might be relevant in vivo for the fibroproliferative response during liver injury.

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Conservation in sequence and affinity of human and rodent PDGF ligands and receptors

Cited by 28 publications

References 38 publications

Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)

Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)

Pentoxifylline Attenuated the Renal Disease Progression in Rats with Remnant Kidney

Crosstalk between PDGF and IGF-I receptors in rat liver myofibroblasts: implication for liver fibrogenesis

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