Conservation of Glutamine-Rich Transactivation Function between Yeast and Humans

Escher, Dominik; Bodmer-Glavas, Morana; Barberis, Alcide; Schaffner, Walter

doi:10.1128/mcb.20.8.2774-2782.2000

Cited by 60 publications

(49 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a relatively conserved transcriptional activation function for the Gln-rich domain from yeast to human (Dynan and Tjian, 1983;Remacle et al, 1997;Escher et al, 2000;Petcherski and Kimble, 2000;Freiman and Tjian, 2002). This might be true for GRP23 as well.…”

Section: Grp23 Encodes a Nuclear Ppr Protein That Is Essential For Eamentioning

confidence: 99%

Arabidopsis GLUTAMINE-RICH PROTEIN23Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III

et al. 2006

View full text Add to dashboard Cite

Precise control of gene expression is critical for embryo development in both animals and plants. We report that Arabidopsis thaliana GLUTAMINE-RICH PROTEIN23 (GRP23) is a pentatricopeptide repeat (PPR) protein that functions as a potential regulator of gene expression during early embryogenesis in Arabidopsis. Loss-of-function mutations of GRP23 caused the arrest of early embryo development. The vast majority of the mutant embryos arrested before the 16-cell dermatogen stage, and none of the grp23 embryos reached the heart stage. In addition, 19% of the mutant embryos displayed aberrant cell division patterns. GRP23 encodes a polypeptide with a Leu zipper domain, nine PPRs at the N terminus, and a Gln-rich C-terminal domain with an unusual WQQ repeat. GRP23 is a nuclear protein that physically interacts with RNA polymerase II subunit III in both yeast and plant cells. GRP23 is expressed in developing embryos up to the heart stage, as revealed by b-glucuronidase reporter gene expression and RNA in situ hybridization. Together, our data suggest that GRP23, by interaction with RNA polymerase II, likely functions as a transcriptional regulator essential for early embryogenesis in Arabidopsis.

show abstract

Section: Grp23 Encodes a Nuclear Ppr Protein That Is Essential For Eamentioning

confidence: 99%

Arabidopsis GLUTAMINE-RICH PROTEIN23Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Surprisingly, no trinucleotide repeats were found exclusively in the exonic regions, although an earlier work by Subramanian et al, (2003) found a twofold greater density of tri repeats in exonic regions compared to the non-coding regions in all human chromosomes except Y. In yeast as well as humans, many proteins involved in transcriptional regulation contain glutaminerich domains and trinucleotide repeats encoding series of polyglutamine (Escher 2000). The repeat length of trimers ranged from 3 to 12 with z20576 locus being the longest (ATC) 12 .…”

Section: Analysis Of Microsatellite Locimentioning

confidence: 59%

In Silico Characterization of Selected Microsatellite Loci Reported in Zebrafish Genome

Pathan¹,

Nesnas²,

Acharya³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…However, this means of innovation is based upon the same sequence characteristics that promote protein--protein and protein-DNA binding (Escher et al 2000;Schaefer et al 2012), such that STR variability must be balanced against functional constraints. This balance has recently been described for a set of 18 coding dinucleotide STRs in humans, which are maintained by natural selection even though any mutation is likely to cause frameshift mutations (Haasl and Payseur 2014).…”

Section: The Pft1 Str Is Required For Pft1 Function In Adult Traitsmentioning

confidence: 99%

“…Specifically, proteasome-dependent degradation of PFT1 is required to activate FT transcription and to promote flowering (Iñigo et al (2012b). The wide range of PFT1-dependent phenotypes is unsurprising given its function in transcription initiation, yet it remains poorly understood how PFT1 integrates these many signaling pathways.Given the conservation of the PFT1 polyQ tract and the known propensity of polyQ tracts for protein-protein and protein-DNA interactions (Escher et al 2000;Schaefer et al 2012), we hypothesized that this polyQ tract plays a role in the integration of multiple signaling pathways and is hence functionally constrained in length. We tested this hypothesis by generating transgenic lines expressing PFT1 with STRs of variable length and evaluating these lines for several PFT1-dependent developmental phenotypes.…”

mentioning

confidence: 99%

“…Given the conservation of the PFT1 polyQ tract and the known propensity of polyQ tracts for protein-protein and protein-DNA interactions (Escher et al 2000;Schaefer et al 2012), we hypothesized that this polyQ tract plays a role in the integration of multiple signaling pathways and is hence functionally constrained in length. We tested this hypothesis by generating transgenic lines expressing PFT1 with STRs of variable length and evaluating these lines for several PFT1-dependent developmental phenotypes.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Conserved PFT1 Tandem Repeat Is Crucial for Proper Flowering in Arabidopsis thaliana

et al. 2014

View full text Add to dashboard Cite

It is widely appreciated that short tandem repeat (STR) variation underlies substantial phenotypic variation in organisms. Some propose that the high mutation rates of STRs in functional genomic regions facilitate evolutionary adaptation. Despite their high mutation rate, some STRs show little to no variation in populations. One such STR occurs in the Arabidopsis thaliana gene PFT1 (MED25), where it encodes an interrupted polyglutamine tract. Although the PFT1 STR is large (270 bp), and thus expected to be extremely variable, it shows only minuscule variation across A. thaliana strains. We hypothesized that the PFT1 STR is under selective constraint, due to previously undescribed roles in PFT1 function. We investigated this hypothesis using plants expressing transgenic PFT1 constructs with either an endogenous STR or synthetic STRs of varying length. Transgenic plants carrying the endogenous PFT1 STR generally performed best in complementing a pft1 null mutant across adult PFT1-dependent traits. In stark contrast, transgenic plants carrying a PFT1 transgene lacking the STR phenocopied a pft1 loss-of-function mutant for flowering time phenotypes and were generally hypomorphic for other traits, establishing the functional importance of this domain. Transgenic plants carrying various synthetic constructs occupied the phenotypic space between wild-type and pft1 loss-of-function mutants. By varying PFT1 STR length, we discovered that PFT1 can act as either an activator or repressor of flowering in a photoperiod-dependent manner. We conclude that the PFT1 STR is constrained to its approximate wild-type length by its various functional requirements. Our study implies that there is strong selection on STRs not only to generate allelic diversity, but also to maintain certain lengths pursuant to optimal molecular function. SHORT tandem repeats (STRs, microsatellites) are ubiquitous and unstable genomic elements that have extremely high mutation rates (Subramanian et al. 2003;Legendre et al. 2007;Eckert and Hile 2009), leading to STR unit number variation within populations. STR variation in coding and regulatory regions can have significant phenotypic consequences (Gemayel et al. 2010). For example, several devastating human diseases, including Huntington's disease and spinocerebellar ataxias, are caused by expanded STR alleles (Hannan 2010). However, STR variation can also confer beneficial phenotypic variation and may facilitate adaptation to new environments (Fondon et al. 2008;Gemayel et al. 2010). For example, in Saccharomyces cerevisiae natural polyQ variation in the FLO1 protein underlies variation in flocculation, which is important for stress resistance and biofilm formation in yeasts (Verstrepen et al. 2005). Natural STR variants of the Arabidopsis thaliana gene ELF3, which encode variable polyQ tracts, can phenocopy elf3 loss-of-function phenotypes in a common reference background (Undurraga et al. 2012). Moreover, the phenotypic effects of ELF3 STR variants differed dramatically between the divergent ...

show abstract

Conservation of Glutamine-Rich Transactivation Function between Yeast and Humans

Cited by 60 publications

References 45 publications

Arabidopsis GLUTAMINE-RICH PROTEIN23Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III

Arabidopsis GLUTAMINE-RICH PROTEIN23Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III

In Silico Characterization of Selected Microsatellite Loci Reported in Zebrafish Genome

The Conserved PFT1 Tandem Repeat Is Crucial for Proper Flowering in Arabidopsis thaliana

Contact Info

Product

Resources

About