Conservation of large foci formation in arrested oocytes of Caenorhabditis nematodes

Jud, Molly C.; Razelun, Jamie; Bickel, Jeremy S.; Czerwinski, Michael; Schisa, Jennifer A

doi:10.1007/s00427-006-0130-3

Cited by 40 publications

(57 citation statements)

References 16 publications

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“…Ovulation arrest induces the formation of RNP granules in the C. elegans gonad [42], [45]. To determine whether VBH-1 changes this expression pattern under this condition, we used fog-2(q71) hermaphrodites.…”

Section: Resultsmentioning

confidence: 99%

The DEAD Box RNA Helicase VBH-1 Is a New Player in the Stress Response in C. elegans

2014

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For several years, DEAD box RNA helicase Vasa (DDX4) has been used as a bona fide germline marker in different organisms. C. elegans VBH-1 is a close homolog of the Vasa protein, which plays an important role in gametogenesis, germ cell survival and embryonic development. Here, we show that VBH-1 protects nematodes from heat shock and oxidative stress. Using the germline-defective mutant glp-4(bn2) we found that a potential somatic expression of vbh-1 might be important for stress survival. We also show that the VBH-1 paralog LAF-1 is important for stress survival, although this protein is not redundant with its counterpart. Furthermore, we observed that the mRNAs of the heat shock proteins hsp-1 and sip-1 are downregulated when vbh-1 or laf-1 are silenced. Previously, we reported that in C. elegans, VBH-1 was primarily expressed in P granules of germ cells and in the cytoplasm of all blastomeres. Here we show that during stress, VBH-1 co-localizes with CGH-1 in large aggregates in the gonad core and oocytes; however, VBH-1 aggregates do not overlap with CGH-1 foci in early embryos under the same conditions. These data demonstrate that, in addition to the previously described role for this protein in the germline, VBH-1 plays an important role during the stress response in C. elegans through the potential direct or indirect regulation of stress response mRNAs.

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Section: Resultsmentioning

confidence: 99%

The DEAD Box RNA Helicase VBH-1 Is a New Player in the Stress Response in C. elegans

2014

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“…In each part, young adult worm is shown above and an older worm below. Redrawn after published observations (12,15,18,24,27,37,48).…”

Section: Aging In the Gonad Armsmentioning

confidence: 99%

“…GEMS AND DE LA GUARDIA that cell density more than doubles (27). Thus, cessation of reproduction leads to hypertrophy in the germline, at least initially.…”

mentioning

confidence: 99%

Alternative Perspectives on Aging inCaenorhabditis elegans: Reactive Oxygen Species or Hyperfunction?

Gems

Guardia

2013

Antioxidants & Redox Signaling

157

122

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Significance: The biological mechanisms at the heart of the aging process are a long-standing mystery. An influential theory has it that aging is the result of an accumulation of molecular damage, caused in particular by reactive oxygen species produced by mitochondria. This theory also predicts that processes that protect against oxidative damage (involving detoxification, repair, and turnover) protect against aging and increase lifespan. Recent Advances: However, recent tests of the oxidative damage theory, many using the short-lived nematode worm Caenorhabditis elegans, have often failed to support the theory. This motivates consideration of alternative models. One new theory, conceived by M.V. Blagosklonny, proposes that aging is caused by hyperfunction, that is, overactivity during adulthood of processes (particularly biosynthetic) that contribute to development and reproduction. Such hyperfunction can lead to hypertrophy-associated pathologies, which cause the age increase in death. Critical Issues: Here we assess whether the hyperfunction theory is at all consistent with what is known about C. elegans aging, and conclude that it is. In particular, during adulthood, C. elegans shows a number of changes that may reflect pathology and/or hyperfunction. Such changes seem to contribute to death, at least in some cases (e.g., yolk accumulation). Future Directions: Our assessment suggests that the hyperfunction theory is a plausible alternative to the molecular damage theory to explain aging in C. elegans. Antioxid. Redox Signal. 19, 321-329.

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“…After the four-cell stage, MEX-3 begins to disappear from the embryo. A small amount of MEX-3 remains in the posterior germline lineage, where it localizes to RNA-rich bodies including germ granules in early embryos and CAR-1/CGH-1 granules in late oocytes (3,(5)(6)(7).…”

mentioning

confidence: 99%

RNA recognition by the embryonic cell fate determinant and germline totipotency factor MEX-3

Pagano

Farley

Essien

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Totipotent stem cells have the potential to differentiate into every cell type. Renewal of totipotent stem cells in the germline and cellular differentiation during early embryogenesis rely upon posttranscriptional regulatory mechanisms. The Caenorhabditis elegans RNA binding protein, MEX-3, plays a key role in both processes. MEX-3 is a maternally-supplied factor that controls the RNA metabolism of transcripts encoding critical cell fate determinants. However, the nucleotide sequence specificity and requirements of MEX-3 mRNA recognition remain unclear. Only a few candidate regulatory targets have been identified, and the full extent of the network of MEX-3 targets is not known. Here, we define the consensus sequence required for MEX-3 RNA recognition and demonstrate that this element is required for MEX-3 dependent regulation of gene expression in live worms. Based on this work, we identify several candidate MEX-3 targets that help explain its dual role in regulating germline stem cell totipotency and embryonic cell fate specification.embryogenesis ͉ nematode ͉ RNA-binding protein ͉ post-transcriptional regulation ͉ maternal effect

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Conservation of large foci formation in arrested oocytes of Caenorhabditis nematodes

Cited by 40 publications

References 16 publications

The DEAD Box RNA Helicase VBH-1 Is a New Player in the Stress Response in C. elegans

The DEAD Box RNA Helicase VBH-1 Is a New Player in the Stress Response in C. elegans

Alternative Perspectives on Aging inCaenorhabditis elegans: Reactive Oxygen Species or Hyperfunction?

RNA recognition by the embryonic cell fate determinant and germline totipotency factor MEX-3

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