Conservation of primary structure in 16S ribosomal RNA

Woese, Carl R.; Fox, George E.; Zablen, L.; Uchida, Tsuneko; Bonen, Linda; Pechman, Kenneth J.; Lewis, B. J.; Stahl, David A.

doi:10.1038/254083a0

Cited by 215 publications

(142 citation statements)

References 22 publications

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“…Identification of the kethoxal-reactive guanines [16] led to the realization that they were among the most phylogenetically conserved sequences in 16S rRNA [18]; indeed, we now know that they are among the most conserved sequences in all of biology. Identification of the tRNAprotected nucleotides by this approach proved unworkable, because the tRNA-binding activity of the uniformly 32 P-labelled ribosomes was lost by autoradiolysis.…”

Section: Chemical Probing Of the 30s P Sitementioning

confidence: 99%

The 30S ribosomal P site: a function of 16S rRNA

Noller¹,

Hoang²,

Fredrick³

2004

FEBS Letters

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The 30S ribosomal P site serves several functions in translation. It must specifically bind initiator tRNA during formation of the 30S initiation complex; bind the anticodon stemloop of peptidyl-tRNA during the elongation phase; and help to maintain the translational reading frame when the A site is unoccupied. Early experiments provided evidence that 16S rRNA was an important component of the 30S P site. Footprinting and crosslinking studies later implicated specific nucleotides in interactions with tRNA. The crystal structures of the 30S subunit and 70S ribosome-tRNA complexes confirmed the interactions between 16S rRNA and tRNA, but also revealed contacts between tRNA and the C-terminal tails of proteins S9 and S13. Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells.

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Section: Chemical Probing Of the 30s P Sitementioning

confidence: 99%

The 30S ribosomal P site: a function of 16S rRNA

Noller¹,

Hoang²,

Fredrick³

2004

FEBS Letters

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“…The primary structures of these rRNA molecules are sufficiently constrained that on the whole they have not changed rapidly in time. Moreover, they contain regions of both extreme conservation (4, 19) and hypervariablity (16,19) so that both distant and close relationships can be examined. Since three distinct rRNA molecules exist, one can ultimately examine the extent to which a particular classification is dependent on the choice of RNA molecule.…”

mentioning

confidence: 99%

Comparative Cataloging of 16S Ribosomal Ribonucleic Acid: Molecular Approach to Procaryotic Systematics

Fox

Pechman

Woese

1977

International Journal of Systematic and Evolutionary Microbiology

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347

139

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“…This is based on nucleotide sequence analyses of rRNA genes, which all cellular organisms possess (Woese et al, 1975). On this basis and supported by other observations, the first common ancestor dates back to ~ 3.7 billion years.…”

Section: Introductionmentioning

confidence: 64%