Conserved and acquired features of<i>neurogenin1</i>regulation

Blader, Patrick; Lam, Chen Sok; Rastegar, Sepand; Scardigli, Raffaella; Nicod, Jean-Christophe; Simplicio, Nicolas; Plessy, Charles; Fischer, Nadine; Schuurmans, Carol; Guillemot, François; Strähle, Uwe

doi:10.1242/dev.01455

Cited by 65 publications

(64 citation statements)

References 54 publications

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“…The responsiveness of the zER81 regulatory elements to regional cues is reminiscent of the restricted activity of a conserved region in the zebrafish Ngn1 promoter, which is sufficient to drive expression in the lateral pallium of mouse embryos in a Pax6-dependent manner (Blader et al, 2004). Strikingly, we found a conserved Pax6 binding site located within the 2-and 1.5-kb zER81 fragments (between 1,315 and 1,294 bp; Fig.…”

Section: Pallial Regulatory Elements From Zebrafish Er81 Are Active Imentioning

confidence: 71%

“…Indeed, only a single published report has examined the activity of pallial-regulatory elements across species. This study investigated the regulatory elements surrounding the proneural gene Ngn1, providing important insights concerning pallial evolution (Blader et al, 2004). Most notably, this study revealed that the expression of Ngn1 in the zebrafish and mouse pallium is dependent on cisregulatory elements that have been conserved at the sequence level, but whose functions have been independently modified during vertebrate evolution.…”

Section: Pallial Regulatory Elements From Zebrafish Er81 Are Active Imentioning

confidence: 99%

“…It is important to consider, however, that genes operate in networks and not in isolation to specify phenotypes (i.e., structure) and apparently similar patterns of gene expression can obscure evolutionary changes in gene regulation. Indeed, the conserved pallial-specific expression of Ngn1 in dividing progenitor cells in the zebrafish and mouse telencephalon requires the activity of cis-regulatory elements that have been conserved at the sequence level, but whose functions have been independently modified during vertebrate evolution (Blader et al, 2004). As such, to assess relationships between genes and structures fully, it is necessary to consider how gene regulatory networks have evolved.…”

Section: Introductionmentioning

confidence: 99%

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Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

Langevin

Mattar

Scardigli

et al. 2007

Developmental Dynamics

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With the ultimate goal of understanding how genetic modules have evolved in the telencephalon, we set out to modernize the functional analysis of cross-species cis-regulatory elements in mouse. In utero electroporation is rapidly replacing transgenesis as the method of choice for gain-and loss-of-function studies in the murine telencephalon, but the application of this technique to the analysis of transcriptional regulation has yet to be fully explored and exploited. To empirically define the developmental stages required to target specific populations of neurons in the dorsal telencephalon, or pallium, which gives rise to the neocortex in mouse, we performed a temporal and spatial analysis of the migratory properties of electroporated versus birth-dated cells. Next, we compared the activities of two known Ngn2 enhancers via transgenesis and in utero electroporation, demonstrating that the latter technique more faithfully reports the endogenous telencephalic expression pattern observed in an Ngn2lacZ knock-in line. Finally, we used this approach to test the telencephalic activities of a series of deletion constructs comprised of the zebrafish ER81 upstream regulatory region, allowing us to identify a previously uncharacterized enhancer that displays cross-species activity in the murine piriform cortex and lateral neocortex, yet not in more medial domains of the forebrain. Taken together, our data supports the contention that in utero technology can be exploited to rapidly examine the architecture and evolution of pallial-specific cis-regulatory elements.

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Section: Pallial Regulatory Elements From Zebrafish Er81 Are Active Imentioning

confidence: 71%

Section: Pallial Regulatory Elements From Zebrafish Er81 Are Active Imentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

Langevin

Mattar

Scardigli

et al. 2007

Developmental Dynamics

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“…Interestingly, all of these genes, except NeuroD, genetically require Pax6 (Marquardt et al, 2001;Blader et al, 2004). The activation of Ngn1 and Ngn2 is directly regulated by Pax6 binding to particular CNS enhancers (Marquardt et al, 2001;Scardigli et al, 2003;Blader et al, 2004).…”

Section: Regulatory Conservation In the Evolutionary Divergence Of Bhmentioning

confidence: 99%

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Hufnagel

Riesenberg

Saul

et al. 2007

Molecular and Cellular Neuroscience

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Retinal ganglion cell genesis requires the proneural bHLH transcription factor Math5 (Atoh7), but little is known about the regulatory elements that control its expression. Here, we investigate Math5 gene regulation using transgenic mice. These mice express GFP in the prenatal retina, livelabeling RGC axon migration and innervation of the brain. Unexpectedly, these Math5-GFP transgenes are also found in Math1 expression domains throughout the nervous system, intriguing since Math5 and Math1 normally exhibit nonoverlapping expression. Furthermore, Math5-GFP and Math1 are regulated similarly, by both Pax6 and Math1 itself, in the lower rhombic lip and dorsal spinal cord. We also show that Pax6 binds to particular Math5 and Math1 regulatory sequences in vitro. Together these data suggest that these atonal semi-orthologues may share conserved regulatory elements that are normally silent in the Math5 gene.

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“…Only after neuronogenesis completion, proneural factors disappear, so allowing astrocytespecific transcriptions to rise (Sun et al, 2001). This capability of Ngns to antagonize astroglial transcriptions might be the main mechanism underlying exaggerated generation of astrocytes, which has been recently found in cultures of pallial precursors knock-out for Pax6 (Sakayori et al, 2012), namely a direct transactivator of Neurog1 and Neurog2 promoters (Scardigli, 2003;Blader, 2004). …”

Section: Acting Downstream Of or In Parallel With Pstat3mentioning

confidence: 86%

Developmental control of cortico-cerebral astrogenesis

Mallamaci¹

2013

Int. J. Dev. Biol.

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A remarkable body of research over the last 15 years has been aimed at disentangling the cellular and molecular mechanisms which regulate murine cortico-cerebral astrogenesis. This research effort has allowed the reconstruction of the actual sizing of this process, as well as a better definition of its temporal, spatial and clonal articulation. Moreover, these investigations have shed substantial light on the cardinal molecular mechanisms governing the transition from pallial neuronogenesis to astrogenesis, as well as subsequent progress of the latter. It has turned out that proper temporal articulation of astrogenesis relies on a plethora of tightly interlaced mechanisms, which synergistically dampen astrogenesis prior to birth and promote it during peri-and postnatal life. The aim of this review is to provide a comprehensive and organic synthesis of these mechanisms, as well as a critical evaluation of their specific relevance to proper articulation of cerebral cortex astrogenesis in time and space.

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Conserved and acquired features ofneurogenin1regulation

Abstract: doi:10.1242/dev.0145

Cited by 65 publications

References 54 publications

Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon

Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Developmental control of cortico-cerebral astrogenesis

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