Conserved Cu-MicroRNAs in Arabidopsis thaliana Function in Copper Economy under Deficiency

Shahbaz, Muhammad; Pilon, Marinus

doi:10.3390/plants8060141

Cited by 27 publications

(16 citation statements)

References 57 publications

(111 reference statements)

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“…Cu deficiency does not alter the expression level of the PC genes but instead triggers a large molecular remodeling that allows Cu to be preferentially allocated to PC [58][59][60]. In order to achieve this, transcripts of Cu/ZnSOD undergo microRNA-mediated downregulation.…”

Section: Chloroplast Responses To Environmental Transition Metal Deficiencymentioning

confidence: 99%

Chloroplast Transition Metal Regulation for Efficient Photosynthesis

Schmidt

Eisenhut

Schneider

2020

Trends in Plant Science

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Plants require sunlight, water, CO 2 , and essential nutrients to drive photosynthesis and fulfill their life cycle. The photosynthetic apparatus resides in chloroplasts and fundamentally relies on transition metals as catalysts and cofactors. Accordingly, chloroplasts are particularly rich in iron (Fe), manganese (Mn), and copper (Cu). Owing to their redox properties, those metals need to be carefully balanced within the cell. However, the regulation of transition metal homeostasis in chloroplasts is poorly understood. With the availability of the arabidopsis genome information and membrane protein databases, a wider catalogue for searching chloroplast metal transporters has considerably advanced the study of transition metal regulation. This review provides an updated overview of the chloroplast transition metal requirements and the transporters involved for efficient photosynthesis in higher plants. Transition Metal Transporters Are Central to Chloroplast Metal Regulation and PhotosynthesisChloroplasts harbor three types of membranes: a double (inner and outer) envelope and a thylakoid membrane. The envelope and thylakoid are separated by the stroma compartment. The membranes, and the transporters embedded therein, are selective barriers enabling exchange of ions and metabolites between the cytosol, the stroma, and the thylakoid lumen compartment. Chloroplast functioning and efficient photosynthesis for plant growth rely on the metal cofactormediated electron transport chain, in particular the transition metals Fe, Mn, and Cu [1]. However, as ions, their chemical properties can lead to generation of undesired reactive oxygen species (ROS) [2], which, together with their different binding affinities to proteins [3], greatly challenge the use of metals in oxygenic photosynthesis. To avoid harmful generation of ROS, plants chelate metal ions by organic ligands, such as nicotianamine, phenolic compounds, or organic acids (e.g., citrate, malate, ascorbate) [4]. Thus, the transport, homeostasis, and regulation of the individual transition metals are fundamental to optimizing chloroplast functioning.

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Section: Chloroplast Responses To Environmental Transition Metal Deficiencymentioning

confidence: 99%

Chloroplast Transition Metal Regulation for Efficient Photosynthesis

Schmidt

Eisenhut

Schneider

2020

Trends in Plant Science

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“…The regulation of copper uptake and internal distribution include transporters from CTR/COPT and Yellow Stripelike (YSL) families (Burkhead et al, 2009). Copper economy/metal switch mechanism involves the increased expression of copper-responsive microRNAs, including miR397, miR398, miR408, and miR857 that, in turn, facilitate the mRNA degradation of copper-containing proteins such as Cu/Zn-superoxide dismutases (SODs), plantacyanin and laccase-like multicopper oxidases (Abdel-Ghany and Pilon, 2008;Pilon, 2017;Shahbaz and Pilon, 2019). In A. thaliana, copper homeostasis is controlled by a conserved transcription factor, SPL7 (Squamosa Promoter Binding Protein-like7), and a recently discovered transcription factor, CITF1 (Copper Deficiency Induced Transcription Factor 1) (Bernal et al, 2012;Kropat et al, 2005;Yamasaki et al, 2009;Yan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Copper deficiency alters shoot architecture and reduces fertility of both gynoecium and androecium inArabidopsis thaliana

Ishka

Vatamaniuk

2020

Preprint

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Date of submission: 6/11/2020 Number of tables: 2 Number of figures: 8 all in color online only Word count: 8157 words including 690 words related to references cited within the text Number of supplementary tables: 1 Number of supplementary figures: 2 Highlight Copper deficiency alters shoot architecture, delays flowering and senescence, and compromises fertility by altering stigma morphology, disrupting anther lignification and dehiscence, and pollen redox status in Arabidopsis thaliana. 3 Abstract Copper deficiency reduces plant growth, male fertility and seed set. The contribution of copper to female fertility and the underlying molecular aspects of copper deficiency-caused phenotypes are not well-known. We show that among copper deficiency-caused defects in Arabidopsis thaliana were the increased shoot branching, delayed flowering and senescence, and entirely abolished gynoecium fertility. The increased shoot branching of copper-deficient plants was rescued by the exogenous application of auxin or copper. The delayed flowering was associated with the decreased expression of the floral activator, FT. Copper deficiency also decreased the expression of senescence-associated genes, WRKY53 and SAG13, but increased the expression of SAG12. The reduced fertility of copper-deficient plants stemmed from multiple factors including the abnormal stigma papillae development, the abolished gynoecium fertility, and the failure of anthers to dehisce. The latter defect was associated with reduced lignification, the upregulation of copper microRNAs and the downregulation of their targets, laccases, implicated in lignin synthesis. Copper-deficient plants accumulated ROS in pollen and had reduced cytochrome c oxidase activity in leaves. This study opens new avenues for the investigation into the relationship between copper homeostasis, hormone-mediated shoot architecture, gynoecium fertility and copper deficiency-derived nutritional signals leading to the delay in flowering and senescence.

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“…The regulation of copper uptake and internal distribution include transporters from CTR/COPT and Yellow Stripe-like (YSL) families (Burkhead et al, 2009). Copper economy/metal switch mechanism involves the increased expression of copper-responsive microRNAs, including miR397, miR398, miR408, and miR857 that, in turn, facilitates the mRNA degradation of copper-containing proteins such as Cu/Zn-superoxide dismutases (SODs), plantacyanin and laccase-like multicopper oxidases (Abdel-Ghany & Pilon, 2008;Pilon, 2017;Shahbaz & Pilon, 2019). In A. thaliana, copper homeostasis is controlled by a conserved transcription factor, SPL7…”

mentioning

confidence: 99%