Conserved MicroRNAs in Chinese hamster ovary cell lines

Abstract

MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.

Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7

“…The TLDAs are designed for profiling human samples but the high degree of evolutionary conservation demonstrated amongst miRNAs made it likely that they would be suitable for CHO profiling also. Indeed, recent information coming from next-generation sequencing of CHO miRNAs indicates that the conservation observed across other species is similarly maintained in hamster (Johnson et al, 2010). In our experiments we detected a similar percentage of the total (∼350) miRNAs present in CHO-derived RNA as we did in human samples (35-42%).…”

Generation of monoclonal antibody-producing mammalian cell lines

“…Use of miRNA in CHO cells is a recent cell engineering technique first reported in 2007 [68]. The number of identified CHO miRNAs has increased exponentially since that report [69][70][71]. Although more studies still need to be conducted using mAb-producing cell lines to verify the usefulness of miRNAs, existing reports are promising.…”

Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data

“…In 2011, the first systematic approaches to miRNA annotation in CHO cells were reported (Hackl et al, 2011; Johnson et al, 2011). Subsequent studies were focused on providing insights into the importance of miRNAs for molecular pathways relevant to cell culture engineering, such as growth (Jadhav et al, 2014), apoptosis (Druz et al, 2011), and protein secretion (Barron et al, 2011; Loh et al, 2014).…”