Conserved N-terminal Regulation of the ACA8 Calcium Pump with Two Calmodulin Binding Sites
Sigrid Thirup Larsen,
Josephine Karlsen Dannersø,
Christine Juul Fælled Nielsen
et al.
Abstract:The autoinhibited plasma membrane calcium ATPase, ACA8 fromA. thalianahas an N-terminal autoinhibitory domain. Calcium-bound calmodulin binding at two sites located at residues 42-62 and 74-96 relieves autoinhibition of ACA8 activity.We investigated N-terminally truncated ACA8 constructs (WT, Δ20, Δ30, Δ35, Δ37, Δ40, Δ74 and Δ100) to explore the role of conserved motifs in the N-terminal segment preceding the calmodulin binding sites. Furthermore, we purified WT, Δ20- and Δ100-ACA8, tested activityin vitroand … Show more
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