Consideration of Sex Differences in Design and Reporting of Experimental Arterial Pathology Studies—Statement From ATVB Council

Robinet, Peggy; Milewicz, Dianna M.; Cassis, Lisa A.; Leeper, Nicholas J.; Lü, Hong; Smith, Jonathan

doi:10.1161/atvbaha.117.309524

Cited by 230 publications

(141 citation statements)

References 142 publications

Supporting

Mentioning

136

Contrasting

Order By: Relevance

“…First, we compared the size of lesions (plaques; revealed by Oil red O staining) in aortic root cross sections prepared from the two groups of mice (KO and control). Prior studies using the mouse model of HFD‐induced atherosclerosis on the C57BL/6 ApoE −/− background showed differences in the disease susceptibilities between males and females 20,21 . However, in our work, differences between the control and L13a KO mice on the C57BL/6 ApoE −/− background with HFD treatment were equivalent for both genders 11 .…”

Section: Resultscontrasting

confidence: 59%

High‐fat diet‐induced GAIT element‐mediated translational silencing of mRNAs encoding inflammatory proteins in macrophage protects against atherosclerosis

et al. 2020

View full text Add to dashboard Cite

Previously, we identified a mechanism of inflammation control directed by ribosomal protein L13a and "GAIT" (Gamma Activated Inhibitor of Translation) elements in target mRNAs and showed that its elimination in myeloid cell-specific L13a knockout mice (L13a KO) increased atherosclerosis susceptibility and severity. Here, we investigated the mechanistic basis of this endogenous defense against atherosclerosis. We compared molecular and cellular aspects of atherosclerosis in high-fat diet (HFD)-fed L13a KO and intact (control) mice. HFD treatment of control mice induced release of L13a from 60S ribosome, formation of RNA-binding complex, and subsequent GAIT element-mediated translational silencing. Atherosclerotic plaques from HFD-treated KO mice showed increased infiltration of M1 type inflammatory macrophages. Macrophages from KO mice showed increased phagocytic activity and elevated expression of LDL receptor and pro-inflammatory mediators. NanoString analysis of the plaques from KO mice showed upregulation of a number of mRNAs encoding inflammatory proteins. Bioinformatics analysis suggests the presence of the potential GAIT elements in the 3′UTRs of several of these mRNAs. Macrophage induces L13a/GAIT-dependent translational silencing of inflammatory genes in response to HFD as an endogenous defense against atherosclerosis in ApoE −/− model. K E Y W O R D S atherosclerosis, GAIT, inflammation, L13a, translational control

show abstract

Section: Resultscontrasting

confidence: 59%

High‐fat diet‐induced GAIT element‐mediated translational silencing of mRNAs encoding inflammatory proteins in macrophage protects against atherosclerosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In this mouse model, suprarenal AAAs form in up to 80–85% of the cases. As indicated in Robinet et al., there is a low AAA incidence in Ang II infused female ApoE −/− mice (∼20%), therefore we chose to study male ApoE −/− mice that have between 80 and 100% AAA incidence [ 7 ]. Both sex hormones [ 8 ] and sex chromosomes [ 9 ] have been shown to effect AAA development in experimental AAA.…”

Section: Methodsmentioning

confidence: 99%

Effect of irradiation and bone marrow transplantation on angiotensin II-induced aortic inflammation in ApoE knockout mice

et al. 2018

View full text Add to dashboard Cite

Background and aimsAngiotensin II (Ang II) infusion promotes the development of aortic aneurysms and accelerates atherosclerosis in ApoE−/− mice. In order to elucidate the role of hematopoietic cells in these pathologies, irradiation and bone marrow transplantation (BMT) are commonly utilized. The aim of this study was to investigate the effects of irradiation and BMT on abdominal and thoracic aortic aneurysm formation and acute leukocyte recruitment in the aortic root and descending aorta, in an experimental mouse model of aortic aneurysm formation.MethodsApoE−/− mice were either lethally irradiated and reconstituted with ApoE−/− bone marrow or non-irradiated. Following engraftment, mice were treated with Ang II to induce aortic inflammation and accelerate atherosclerosis.ResultsAng II infusion (0.8 mg/kg/day) in BMT mice resulted in reduced aortic aneurysms and atherosclerosis with decreased leukocyte infiltration in the aorta compared to non-BMT mice, when receiving the same dose of Ang II. Furthermore, the reduced aortic infiltration in BMT mice was accompanied by increased levels of monocytes in the spleen and bone marrow. A dose of 3 mg/kg/day Ang II was required to achieve a similar incidence of aneurysm formation as achieved with 0.8 mg/kg/day in non-BMT mice.ConclusionsThis study provides evidence that BMT can alter inflammatory cell recruitment in experimental mouse models of aortic aneurysm formation and atherosclerosis and suggests that irradiation and BMT have a considerably more complex effect on vascular inflammation, which should be evaluated.

show abstract

“…Early population-based estimates of PAD prevalence in women have been plagued by methodological difficulties, including the use of intermittent claudication as the basis for disease categorization and failure to account for mean height differences between men and women when determining normal ABI values. 90 The prevalence of PAD increases with age for both men and women; however, when calculating the "burden" of disease by age and sex from US census data, there are more women than men with PAD among US adults ≥40 years of age. 91 Contemporary reviews now estimate that the global prevalence of PAD is higher in women than in men, 92 and the increase in mortality and disability associated with PAD worldwide has been greater among women than men.…”

Section: Opportunities To Identify Differences Related To Sex and Ethmentioning

confidence: 99%

Perfusion Assessment in Critical Limb Ischemia: Principles for Understanding and the Development of Evidence and Evaluation of Devices: A Scientific Statement From the American Heart Association

Misra¹,

Shishehbor²,

Takahashi³

et al. 2019

Circulation

103

View full text Add to dashboard Cite

There are >12 million patients with peripheral artery disease in the United States. The most severe form of peripheral artery disease is critical limb ischemia (CLI). The diagnosis and management of CLI is often challenging. Ethnic differences in comorbidities and presentation of CLI exist. Compared with white patients, black and Hispanic patients have higher prevalence rates of diabetes mellitus and chronic renal disease and are more likely to present with gangrene, whereas white patients are more likely to present with ulcers and rest pain. A thorough evaluation of limb perfusion is important in the diagnosis of CLI because it can not only enable timely diagnosis but also reduce unnecessary invasive procedures in patients with adequate blood flow or among those with other causes for ulcers, including venous, neuropathic, or pressure changes. This scientific statement discusses the current tests and technologies for noninvasive assessment of limb perfusion, including the ankle-brachial index, toe-brachial index, and other perfusion technologies. In addition, limitations of the current technologies along with opportunities for improvement, research, and reducing disparities in health care for patients with CLI are discussed.

show abstract

Consideration of Sex Differences in Design and Reporting of Experimental Arterial Pathology Studies—Statement From ATVB Council

Cited by 230 publications

References 142 publications

High‐fat diet‐induced GAIT element‐mediated translational silencing of mRNAs encoding inflammatory proteins in macrophage protects against atherosclerosis

High‐fat diet‐induced GAIT element‐mediated translational silencing of mRNAs encoding inflammatory proteins in macrophage protects against atherosclerosis

Effect of irradiation and bone marrow transplantation on angiotensin II-induced aortic inflammation in ApoE knockout mice

Perfusion Assessment in Critical Limb Ischemia: Principles for Understanding and the Development of Evidence and Evaluation of Devices: A Scientific Statement From the American Heart Association

Contact Info

Product

Resources

About