Considerations and Regulatory Approaches in the USA and in the EU for dsRNA-Based Externally Applied Pesticides for Plant Protection

Dietz‐Pfeilstetter, Antje; Mendelsohn, Mike; Gathmann, Achim; Klinkenbuß, Dominik

doi:10.3389/fpls.2021.682387

Cited by 35 publications

(29 citation statements)

References 44 publications

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“…It can help control this insect pest through two modes of application: non-transformative (bioproduct) and transformative (transgenic plants) delivery. The non-transformative delivery presents some advantages for use in pest management because of the rapid product development, low cost, low resistance risk, and high viability for all crops [87], although there are no stringent regulations in different countries [88].…”

Section: Primary Considerations and Future Prospects Of Rnai Strategies For Acp Control In Citrus Orchardsmentioning

confidence: 99%

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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Citrus species, including sweet oranges, grapefruits, pomelos, and lemons, are the most widely cultivated trees and consumed fruits worldwide. In citrus orchard management, the control of Huanglongbing (HLB) disease and its insect vector Diaphorina citri (Asian citrus psyllid, ACP) represents a major global challenge. Consumers have been increasingly pushing the citrus production chain toward a more sustainable system, including stringent measures to prevent the use of chemical pesticides. In recent years, biotechnological advances have offered safe and environmentally friendly alternatives for crop production. Technologies such as RNA interference (RNAi)-mediated gene silencing have emerged as innovative tools for agricultural pest management. Here, we provide an overview of RNAi as a promising approach for ACP control and discuss the associated challenges. Despite the availability of specific silencing sequences aimed at a target gene of the insect pest, the uptake of double-stranded RNA is limited in hemipteran insects. In this context, improved delivery methods, stability maintenance, and RNAi response are considered the factors contributing to the increased effectiveness of exogenous RNAi against hemipteran pests. These approaches can serve as potential tools for efficient ACP control.

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Section: Primary Considerations and Future Prospects Of Rnai Strategies For Acp Control In Citrus Orchardsmentioning

confidence: 99%

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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“…Then, appropriate management, supervision, and legislation will be necessary, and RNAibased products must go through appropriate safety evaluation and approval processes. Sprayed or externally applied dsRNA-based pesticides are under development, but there are currently no legal regulations specific to such RNAi-based pesticides [112]. There are no specific guidance documents defining the data requirements for the approval of dsRNA-based pesticides in the European Union or the United States.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture

Hashiro

Yasueda

2022

Applied Sciences

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RNA interference (RNAi)-based pesticides are pest control agents that use RNAi mechanisms as the basis of their action. They are regarded as environmentally friendly and are a promising alternative to conventional chemical pesticides. The effective substance in RNAi-based pesticides is double-stranded RNA (dsRNA) designed to match the nucleotide sequence of a target essential gene of the pest of concern. When taken up by the pest, this exerts an RNAi effect and inhibits some vital biochemical/biological process in the pest. dsRNA products are also expected to be applied for the control of viral diseases in aquaculture by RNAi, especially in shrimp farming. A critical issue in the practical application of RNAi agents is that production of the dsRNA must be low-cost. Here, we review recent methods for microbial production of dsRNAs using representative microorganisms (Escherichia coli, Pseudomonas syringae, Corynebacterium glutamicum, Chlamydomonas reinhardtii, and others) as host strains. The characteristics of each dsRNA production system are discussed.

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“…However, lab-to-field transitions require further precision and accuracy in off-target predictions and their controllability for adequate risk assessment. Currently, data requirements specific for RNA spray-based plant protection products are not yet in place under the regulatory frameworks for pesticides and plant protection products in the United States and EU (Dietz-Pfeilstetter et al, 2021). The OECD (Organization for Economic Cooperation and Development) organized a meeting on this question, which represented a major step forward in terms of testing regulation and research direction for future external dsRNA products (OECD, 2020).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Lab-to-Field Transition of RNA Spray Applications – How Far Are We?

Rank

Koch

2021

Front. Plant Sci.

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The drastic loss of biodiversity has alarmed the public and raised sociopolitical demand for chemical pesticide-free plant production, which is now treated by governments worldwide as a top priority. Given this global challenge, RNAi-based technologies are rapidly evolving as a promising substitute to conventional chemical pesticides. Primarily, genetically modified (GM) crops expressing double-stranded (ds)RNA-mediating gene silencing of foreign transcripts have been developed. However, since the cultivation of GM RNAi crops is viewed negatively in numerous countries, GM-free exogenous RNA spray applications attract tremendous scientific and political interest. The sudden rise in demand for pesticide alternatives has boosted research on sprayable RNA biopesticides, generating significant technological developments and advancing the potential for field applications in the near future. Here we review the latest advances that could pave the way for a quick lab-to-field transition for RNA sprays, which, as safe, selective, broadly applicable, and cost-effective biopesticides, represent an innovation in sustainable crop production. Given these latest advances, we further discuss technological limitations, knowledge gaps in the research, safety concerns and regulatory requirements that need to be considered and addressed before RNA sprays can become a reliable and realistic agricultural approach.

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Considerations and Regulatory Approaches in the USA and in the EU for dsRNA-Based Externally Applied Pesticides for Plant Protection

Cited by 35 publications

References 44 publications

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture

Lab-to-Field Transition of RNA Spray Applications – How Far Are We?

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