The cornea, a transparent tissue composed of multiple layers, allows light to enter the eye. Several single-cell RNA-seq analyses have been performed to explore the cell states and to understand the cellular composition of the human cornea. However, the inconsistences in cell state annotations between these studies complicate the application of these findings in corneal studies. To address this, we integrated single-cell RNA-seq data from four published studies and created a human corneal cell state meta-atlas. This meta-atlas was subsequently evaluated in two applications. First, we developed a machine learning pipeline cPredictor, using the human corneal cell state meta-atlas as input, to annotate corneal cell states. We demonstrated the accuracy of cPredictor and its ability to identify novel marker genes and rare cell states in the human cornea. Furthermore, cPredictor revealed the differences of the cell states between pluripotent stem cell-derived corneal organoids and the human cornea. Second, we integrated the single-cell RNA-seq based cell state meta-atlas with chromatin accessibility data, conducting motif-focused and gene regulatory network analyses. These approaches identified distinct transcription factors driving cell states of the human cornea. The novel marker genes and transcription factors were validated by immunohistochemistry. Overall, this study offers a reliable and accessible reference for profiling corneal cell states, which facilitates future research in cornea development, disease and regeneration.Significance statementThis study creates a human corneal cell state meta-atlas that provides a common nomenclature of cells in the human cornea, through integrating multiple single-cell RNA-seq analyses. Using this meta-atlas, we developed a machine learning pipeline, cPredictor, to accurately annotate cell states in corneal studies using single-cell RNA-seq. Additionally, we identified distinct transcription factors driving cell states through integrating the atlas with chromatin accessibility data. This meta-atlas and the computational tool cPredictor enable future research in cornea development, disease, and regeneration.
