Considerations in Development and Validation of LC‐MS/MS Method for Quantitative Analysis of Small Molecules in Dried Blood Spot Samples

Li, Wenkui

doi:10.1002/9781118890837.ch14

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…After repeating the extraction step twice, the extract is centrifuged, and the supernatant is collected, dried in a concentrator, reconstituted in a suitable solvent, and then analyzed by using a chromatography instrument [53]. Most often, GC-MS/MS, UPLC, or LC-MS/MS are used in the DBS analysis [54][55][56].…”

Section: Dried Blood Spot (Dbs)mentioning

confidence: 99%

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Srivastava¹,

Godbole²,

Shrivastava³

2022

austinjanalpharmchem

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Steroid profiling plays an important role in the clinical setting and also in the diagnosis of various physiological disorders. Steroids in the research laboratory and patient care has been routinely measured by immunoassay. Limitations of immunoassay in quantifying steroids have been well documented and the advent of advanced mass spectrometry is offering a viable alternative to measure multiple steroids in single reaction. Analytes ranging from the steroid and their metabolites that are present in the body carry out various important functions and are relevant to maintaining homeostasis. Blood plasma and urine samples are the clinical material for such analysis, however, due to the difference in polarity of the target steroid, a major challenge exists as to how faithfully analyze different steroids in the given sample type. These steroids are usually present at very low concentrations in the body and are present in several forms leading to increased complexity. Achieving an excellent chromatographic separation for the analysis of steroids requires an effective sample preparation and analysis procedure. Hence various methods are developed to analyze the presence of steroids and to know their exact concentration in the body. This review covers various analytical methodologies such as chromatographic procedures and downstream mass spectrometry for the identification and measurement of the levels of steroid hormones. At the same time, this review also covers the most recent developments that have taken place in the recent past to cover the enhanced understanding of steroid hormone analysis.

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Section: Dried Blood Spot (Dbs)mentioning

confidence: 99%

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Srivastava¹,

Godbole²,

Shrivastava³

2022

austinjanalpharmchem

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“…Dried Blood Spots (DBS) is a blood collection method on a paper card that has been used for decades for the serologic diagnosis of infectious diseases, especially in resource-limited settings [24][25][26][27][28][29][30][31]. Blood is easily collected by a single finger-prick using a lancet, and self-collected blood on DBS cards can be transported via regular mail from diverse locations and is relatively inexpensive.…”

Section: Introductionmentioning

confidence: 99%

Feasibility and Acceptability of Self-Collected Dried Blood Spots for SARS-CoV-2 Vaccine Response in Community-Dwelling Elderly: A Large Decentralized Prospective Study

2023

J Community Med Public Health

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Background:The STOP-CoV study is an ongoing longitudinal decentralized cohort study assessing the safety and efficacy of the SARS-CoV-2 vaccine. There are limited longitudinal reports on the use of self-collected dried blood spots (DBS) in the elderly population to assess COVID immunity. Objective: To evaluate the feasibility and acceptability of self-collected dried blood spots (DBS) and assess participant characteristics associated with completion rates and specimen adequacy. Methods: 1286 ambulatory adults, including 911 older (70+ years old) and 375 younger (30-50 years) were recruited in Ontario, Canada, between May and July 2021. DBS were requested every three months after the initial vaccine series and 3-4 weeks after vaccine boosters. Results: Of the participants, 2 did not meet screening criteria and 79 consenting participants did not complete any study activities. Among the remaining 1205, 94.3% submitted at least one DBS, and 68.4% submitted all expected specimens. 98.1% of specimens were submitted within the expected time window, and 93.9% were adequate for serology testing across the study. Higher DBS adequacy rates were observed for females compared to males (OR: 1.60; 95% CI: 1.04-2.47) after adjusting for time, age cohort, race, and level of education. The proportion of specimens that were adequate for testing increased over time. Conclusion: Using self-collected DBS for SARS-CoV-2 vaccine response assessment in the elderly ambulatory community is feasible, acceptable, and resulted in high submission rates, specimen adequacy, and retention over 48 weeks. Remote self-collection of DBS can increase recruitment, engagement, and retention of underrepresented and/or vulnerable communities in research.

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“…However, the benefits of DBS collection compared to conventional venous collection have been increasingly appreciated for applications in other clinical fields. These benefits include minimally invasive sample collection, low cost, minimal sample processing requirements, increased stability during both long-term storage and shipping, reduced risk of bacterial contamination or hemolysis, and the ability to obtain high quality samples from remote locations or from patients with limited mobility 4 – 7 . Recently, application of DBSs in clinical laboratory diagnostics has increased dramatically to include not only newborn screening and infection diagnosis 3 , 8 but also toxicokinetic and pharmacokinetic studies 9 , drug development 10 , therapeutic drug monitoring 11 , clinical pharmacology 2 , forensic toxicology 12 , metabolic profiling 2 , environmental control, epidemiological disease surveillance 2 , 8 , etc.…”

Section: Introductionmentioning

confidence: 99%

Dried blood sample analysis by antibody array across the total testing process

Whittaker

Mao

Lin

et al. 2021

Sci Rep

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Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.

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Considerations in Development and Validation of LC‐MS/MS Method for Quantitative Analysis of Small Molecules in Dried Blood Spot Samples

Cited by 5 publications

References 35 publications

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Feasibility and Acceptability of Self-Collected Dried Blood Spots for SARS-CoV-2 Vaccine Response in Community-Dwelling Elderly: A Large Decentralized Prospective Study

Dried blood sample analysis by antibody array across the total testing process

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