Considerations in Real-time Reverse Transcription Polymerase Chain Reaction (rRT-PCR) for the Detection of SARS-CoV-2 from Nasopharyngeal Swabs

Sekar, Priyadharshini; Menezes, Godfred Antony; Shivappa, Pooja; George, Biji Thomas; Hossain, Ashfaque

doi:10.9734/jpri/2021/v33i1731309

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Most of the time when RT-PCR is negative, the results suggestive of COVID was correlated with the CT scan. 12 We can see multiple ground glass appearance involving both the lobes of the lungs when there is > 50% damage. If you find a 50% increase in lung X-ray findings within 24 to 48 hours, this was considered an early warning indicator of severity of the disease.…”

Section: Discussionmentioning

confidence: 95%

To Determine Role of C-reactive Protein Test and Computerised Tomography Scan of Lung in Diagnosis of COVID-19

Bhagya¹,

Subbalakshmi²,

Devi³

2021

J. Pure Appl. Microbiol.

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The outbreak of novel Coronavirus Disease 2019 (COVID-19) has led the entire world to limp. The virus is causing the severe acute respiratory syndrome, with increasing morbidity and mortality. The WHO declared COVID-19 as a pandemic or public health emergency because of its morbidity and mortality. Several inflammatory markers have been used in the initial diagnosis, as well as prognostic markers in disease progression. One of the inflammatory markers is C reactive protein. The other markers that help in prognosis are ferritin, neutrophil-lymphocyte ratio, CT scan of the lung, IL6 (interleukin 6). RT PCR is reliable diagnostic evidence. Aim of the study: 1. The aim is to determine the C-reactive protein, CT (Computerised Tomography) scan of the lung, RT PCR (Reverse Transcriptase Polymerase Chain reaction) in Covid19 patients. 2. To compare CRP & CT scan values of the lung in various age groups. A cross-sectional study was done from September 2020 to January 2021 and the results were analysed. Out of 704 covid positive patients, the maximum number of patients were in the age group between 31 to 70. The RT PCR showed COVID positive results in 689 (97.8%) patients. The CRP values were found to be >25mg/l from 31-50 (34.7%) years and in 51-70 years of age (52%). In comparison of CT scan with CRP changes, CRP values were found to be raised when there is 25-50% (24.5%) and >50% (27.6%) involvement of lung by COVID. CT scan lung involvement was >50% in the age group between 31-50 (38.9%) and 51-70 (50%), respectively. High CRP values of >25mg/l were observed with the same age group 34.7% with the group of 31-50 and 52% with the group of 51-70, respectively. CRP correlated with the advancing age group and was found to be a good predictor of disease progression.

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Section: Discussionmentioning

confidence: 95%

To Determine Role of C-reactive Protein Test and Computerised Tomography Scan of Lung in Diagnosis of COVID-19

Bhagya¹,

Subbalakshmi²,

Devi³

2021

J. Pure Appl. Microbiol.

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Psychological Impact of COVID-19 Pandemic among Healthcare Personnel in United Arab Emirates

Srivastava,

Menezes,

Kenneth

et al. 2024

J. Pure Appl. Microbiol.

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Given the magnitude of the COVID-19 pandemic, adverse psychological outcomes are expected to occur among healthcare personnel (HCPs). However, little is known about these outcomes within Ras al Khaimah, UAE. This study aims to assess the psychological impact of COVID-19 on healthcare personnel from different hospitals and clinics in Ras Al Khaimah (RAK), UAE. An online questionnaire was distributed to healthcare personnel within Ras al Khaimah, UAE, through an institutional email that enabled them to enroll in the study after giving informed consent. Likert scale was used to calculate the relation between demographic factors and 13 variables of psychological distress. An unpaired t-test was applied. This study shows that 31.7% of HCPs in RAK report moderate to high levels of anxiety due to concerns of potential workplace exposure, 41.6% are afraid of infecting their families and 13.1% find themselves feeling isolated from their families. Those who were younger than 45 years old, unmarried people and having more than two children were more likely to experience psychological distress than their counterparts. While the adverse psychological impact within this population is not as severe as what was noted in different populations in other countries, they must be addressed. It is important to ensure that HCPs have the appropriate physical and mental resources to persevere through similar stressful periods in the future.

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Workplace Safety and Screening of Healthcare Workers for SARS-CoV-2 at a Tertiary Care Hospital in the Northern Emirates of United Arab Emirates

Shivappa,

Parmar,

Menezes

et al. 2024

Journal of Pharmacy and Bioallied Sciences

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Background: Coronavirus disease 2019 (COVID-19) was first reported in December 2019 in Wuhan, People’s Republic of China, and caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), As the virus took hold in the world, health experts paced efforts to solve the unknown nature of this threat. Methodology: We studied the clinical characteristics, laboratory biomarkers of healthcare workers in the single center, analytical cross-sectional study conducted in tertiary care hospital of the UAE. Sample size of 600 HCWs were screened for SARS-CoV-2 by real-time reverse transcription polymerase chain reaction (rRT-PCR) assay using Seegene Allplex and Andis FAST SARS-CoV-2 RT-qPCR detection kits for a period of 6 months. Findings: All the health care workers (HCWs) were vaccinated for the SARS-CoV-2. Out of these, 240 HCWs were reported to be positive. rRT-PCR with a Ct value of <38 was taken as positive >38. The laboratory investigations of the positive cases were collected and analysed further. A comparison of the laboratory tests was made between (I) COVID severe (Ct < 25) and nonsevere (Ct > 25) groups and (II) frontline workers and non-frontline workers. Besides finding short-term and sudden relief, investigators directed their efforts to provide a long-term solution for an ensuing pandemic that influenced all areas of life in unprecedented ways. Conclusion: Awareness among healthcare workers and further long-term studies focusing on their mental health the overall wellness and especially the psychological burden on doctors have received heightened awareness in media and research publications. This information can assist in developing surveillance and infection and control policies and preparedness for future COVID epidemics in similar scenarios in the future.

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Considerations in Real-time Reverse Transcription Polymerase Chain Reaction (rRT-PCR) for the Detection of SARS-CoV-2 from Nasopharyngeal Swabs

Cited by 3 publications

References 32 publications

To Determine Role of C-reactive Protein Test and Computerised Tomography Scan of Lung in Diagnosis of COVID-19

To Determine Role of C-reactive Protein Test and Computerised Tomography Scan of Lung in Diagnosis of COVID-19

Psychological Impact of COVID-19 Pandemic among Healthcare Personnel in United Arab Emirates

Workplace Safety and Screening of Healthcare Workers for SARS-CoV-2 at a Tertiary Care Hospital in the Northern Emirates of United Arab Emirates

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