Considerations in the design of studies of dietary influences on mammary carcinogenesis in rats and mice

Abstract

Design of diets for the study of dietary influences in mammary gland carcinogenesis requires attention to several questions: (1) Do the diets satisfy the nutritional needs of the animal under the conditions of the experiment, and are they palatable? (2) Does the protocol include determination of feed intake (if indicated) and of achievement of the desired level of nutrient deficiency, adequacy, or excess? (3) Are there potentially confounding nutrient interactions or nutrient effects or physiological or pathological responses that must be considered? The particular sensitivity of mammary gland tumorigenesis to intake of fat and calories and to body weight gain must be considered and controlled for in all experiments.

Gut content microbiota dysbiosis and dysregulated lipid metabolism in diarrhea caused by high-fat diet in a fatigued state

“…Colon carcinogenesis was increased in rats fed high-fat diets in certain models. 4 Iwasaki et al 5 found that lard enhanced the large intestinal polygenesis induced by methylazoxymethanol acetate in germ-free mice. Reddy et al 6 found that animals fed with 23.5 and 13.6% lard diet during the initiation stage and 23.5% lard diet during the postinitiation phase developed more colon adenocarcinomas than those fed with 5% lard diet.…”