Consistent chromosome abnormalities in adenocarcinoma of the pancreas

Cytogenetic and FISH analyses of pancreatic carcinoma reveal breaks in 18q11 with consistent loss of 18q12-qter and frequent gain of 18p

“…nullisomy for 18q material was not seen. The observed frequent loss of 18q is in agreement with earlier cytogenetic (Johansson et al, 1992;Bardi et al, 1993;Griffin et al, 1994Griffin et al, , 1995, CGH (Fukushige et al, 1997;Mahlamaki et al, 1997) and LOH studies (Hahn et al, 1995). In the ten cases in which losses of 18q occurred through breaks in the q arm, the breakpoints were localized using both YAC and pcpl8q probes.…”

“…The clustering of breaks close to the centromere indicates that loss of genes in bands 18q11 and 18q12, in addition to those located in 18q21, e.g. DPC4 and DCC, are important in the development of pancreatic tumours.Keywords: chromosome 18; pancreatic carcinoma; deletions Although only a handful of larger series of cytogenetically investigated pancreatic carcinomas have been reported, several frequent recurrent chromosomal imbalances, such as trisomy 7 and 20, monosomy 18, loss of Ip, 3p, 6q, 8p, 9p, 17p and 19p, and gain of lq, 3q, 8q, 1 lq, 19q and 20q, have been identified (Johansson et al, 1992;Bardi et al, 1993;Griffin et al, 1994Griffin et al, , 1995Gorunova et al, 1998). The pattern of cytogenetic imbalances seems characteristic for pancreatic cancer, albeit many of the individual changes are also found in other solid tumours (Mertens et al, 1997).…”

“…In some of the frequently deleted regions, a concomitant inactivation of a tumour-suppressor gene (TSG) on the remaining normal chromosome has been shown, i.e. CDKN2A in 9p2l Bartsch et al, 1995), BRCA2 in 13q12 (Goggins et al, 1996), TP53 in 17pl3 (Ruggeri et al, 1992;Scarpa et al, 1993) and SMAD4 (DPC4) in 18q21 , whereas the TSGs in the other loci remain to be identified.The most common chromosome aberration in pancreatic cancer is monosomy 18 (Johansson et al, 1992;Bardi et al, 1993;Griffin et al, 1994Griffin et al, , 1995. However, recent cytogenetic (Gorunova et al, 1998) and comparative genomic hybridization (CGH) analyses (Mahlamaki et al, 1997) have also revealed frequent partial deletions of 18q.…”

“…The most common chromosome aberration in pancreatic cancer is monosomy 18 (Johansson et al, 1992;Bardi et al, 1993;Griffin et al, 1994Griffin et al, , 1995. However, recent cytogenetic and comparative genomic hybridization (CGH) analyses (Mahlamaki et al, 1997) have also revealed frequent partial deletions of 18q.…”

Chromosomal imbalances in primary and metastatic pancreatic carcinoma as detected by interphase cytogenetics: basic findings and clinical aspects

“…So far, 90 exocrine and endocrine pancreatic cancers have been karyotyped successfully (larger series on exocrine pancreatic carcinoma: Johansson et al, 1992;Bardi et al, 1993;Griffin et al, 1994Griffin et al, , 1995 smaller series or case reports on exocrine or endocrine pancreatic tumours: van der Riet-Fox et al, 1979;Bullerdiek et al, 1985;Casalone et al, 1987;Teyssier, 1987;Scappaticci et al, 1992;Bardi et al, 1994;Bugalho et al, 1994;Danner et al, 1994;Gorunova et al, 1995;Wiley et al, 1995;Grant et al, 1996). Considering the data available, loss of chromosome 18 is the most common numerical aberration identified by metaphase cytogenetics, occurring in half of the exocrine tumours with an abnormal karyotype.…”