Consistent detection of bovine papillomavirus in lesions, intact skin and peripheral blood mononuclear cells of horses affected by hoof canker

Brandt, Sabine; Schoster, Angelika; Tober, Reinhard; Kainzbauer, C.; Burgstaller, Joerg P.; Haralambus, Rhea; Steinborn, Ralf; Hinterhofer, C.; Stanek, C

doi:10.1111/j.2042-3306.2010.00147.x

Cited by 38 publications

(42 citation statements)

References 26 publications

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“…All these data suggest a viral activity in blood cells (Stocco dos Santos et al , 1998; Araldi et al , 2013). The presence of BPV DNA sequences in peripheral blood mononuclear cells (PBMCs) was also described in the literature in both bovines (Roperto et al , 2008; Silva et al , 2013a) and equines (Brandt et al , 2008, 2011), reinforcing our results. Following studies also showed the presence of BPV transcripts and the L1 capsid protein in PBMCs, demonstrating the productive infection in blood cells (Roperto et al.…”

Section: Breaking Paradigmssupporting

confidence: 90%

Papillomaviruses: a systematic review

et al. 2017

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In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.

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Section: Breaking Paradigmssupporting

confidence: 90%

Papillomaviruses: a systematic review

et al. 2017

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“…We have demonstrated the presence of BPV‐1/2 E5 DNA (Brandt et al . 2008) and mRNA (Brandt et al . 2009, 2010) in PBMCs of horses affected by sarcoids or hoof canker.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to EcPV‐2, we detected BPV‐1 DNA in the periorbital/parotideal lesion, the SCC in the jugular groove, in apparently intact skin excised from the flank, and in vulval mucosa. In sarcoid‐ or hoof canker‐affected horses, infection by sarcoid‐inducing BPV‐1/2 is not restricted to the lesion(s) but involves the entire integument and PBMCs (Nasir and Campo 2008; Brandt et al . 2010).…”

Section: Discussionmentioning

confidence: 99%

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Bovine papillomavirus type 1 and Equus caballus papillomavirus 2 in equine squamous cell carcinoma of the head and neck in a Connemara mare

Kainzbauer¹,

Rushton

Tober

et al. 2011

Equine Veterinary Journal

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In January 2010, 18 months after excision of an ocular squamous cell carcinoma (SCC), a Connemara mare presented with anorexia and periorbital/parotideal lesions. Post mortem examination revealed these lesions as forming one entity, with 2 additional growths in the retropharyngeal region and the left jugular groove, respectively. The lesions were confirmed histopathologically as SCCs. Using PCR, peripheral blood mononuclear cells (PBMCs) from 2008 and 2010, tumour tissue, intact skin and vulval mucosa were screened for Equus caballus papillomavirus type 2 (EcPV-2) and bovine papillomavirus types 1 and 2 (BPV-1/2) DNA. Whereas PBMCs from 2008 scored negative, EcPV-2 DNA was present in PBMCs and SCCs from 2010. Furthermore, reverse transcription PCR revealed EcPV-2 E6 transcripts in these samples. BPV-1/2 DNA, but not RNA, was demonstrated in the periorbital/parotideal mass, the SCC of the jugular groove, vulval mucosa and intact skin, but not in the pharyngeal SCC and PBMCs. Sequencing revealed a 99% similarity of EcPV-2 amplicons with the published EcPV-2 sequence. BPV-1/2 amplicons corresponded to BPV type 1. This report is the first to describe co-presence of BPV-1 and EcPV-2 DNA in a pony affected by an uncommon form of nongenital SCC, and the detection of EcPV-2 transcripts in lesions and PBMCs.

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“…2002; O'Grady and Madison 2004; Nagamine et al . 2005; Fürst and Lischer 2006; Brandt et al . 2010).…”

Section: Introductionunclassified

Retrospective study on 30 horses with chronic proliferative pododermatitis (canker)

Oosterlinck

Deneut

Dumoulin

et al. 2011

Equine Veterinary Education

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The medical records of 30 horses (18 Warmbloods, 7 draught horses, 3 other breeds and 2 of unknown origin) with chronic proliferative pododermatitis (canker) were reviewed and long-term outcome was obtained by telephone questionnaire. In 28/30 cases, the owner was the first to discover the problem. The disease was initially recognised as canker in only 5/28 cases, whereas in 10/28 cases a treatment for thrush had been continued for several months before referral. There was a similar prevalence in the fore (41) and hind (44) hooves; 13/30 horses had 4 hooves affected. Treatment consisted of surgical debridement and hoof care. Duration of hospitalisation was significantly decreased in horses receiving oral prednisolone for 3 weeks compared to those without this additional systemic treatment (mean +/- s.d. 24 +/- 5 days, n = 7 vs. 40 +/- 19 days, n = 19, respectively). Long-term follow-up ranged from 3 months to 6 years (36 +/- 22 months) and was available for 24 horses. No recurrence was reported in 10 horses. In 14 horses problems recurred within the first year, and 6 had been subjected to euthanasia for this reason specifically, whereas the others were managed by regular trimming. There was no significant association between recurrence and the number of affected hooves or the use of any systemic treatment. Horses with delayed referral because of preceding treatments had significantly more chance to develop recurrence, highlighting the need for prompt diagnosis and subsequent treatment

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Consistent detection of bovine papillomavirus in lesions, intact skin and peripheral blood mononuclear cells of horses affected by hoof canker

Abstract: The use of antivirals and/or immune modulators may help improving canker therapy.

Cited by 38 publications

References 26 publications

Papillomaviruses: a systematic review

Papillomaviruses: a systematic review

Bovine papillomavirus type 1 and Equus caballus papillomavirus 2 in equine squamous cell carcinoma of the head and neck in a Connemara mare

Retrospective study on 30 horses with chronic proliferative pododermatitis (canker)

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