Consistent repeated M- and H-wave recording in the hind limb of rats

Cliffer, Kenneth D.; Tonra, James R.; Carson, Susan R.; Radley, Heather E.; Cavnor, Chris; Lindsay, Ronald M.; Bodine, Sue C.; DiStefano, Peter S.

doi:10.1002/(sici)1097-4598(199811)21:11<1405::aid-mus7>3.0.co;2-d

Cited by 38 publications

(28 citation statements)

References 33 publications

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“…The H-wave deficits were accompanied by reductions in SNCV, which was also partially prevented by GCPII inhibition. H-wave reflexes provide valuable information about functionally important features of peripheral sensory nerves and their synaptic connections to the central nervous system [48]. Consistent with previous reports that the pyridoxine intoxication selectively targets sensory nerve function [27], [29], we did not find a difference in the amplitude or latency of the motor M wave.…”

Section: Discussionsupporting

confidence: 91%

Glutamate Carboxypeptidase II Inhibition Behaviorally and Physiologically Improves Pyridoxine-Induced Neuropathy in Rats

Potter

Wozniak

Callizot³

et al. 2014

PLoS ONE

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Pyridoxine is used as a supplement for treating conditions such as vitamin deficiency as well as neurological disorders such as depression, epilepsy and autism. A significant neurologic complication of pyridoxine therapy is peripheral neuropathy thought to be a result of long-term and high dose usage. Although pyridoxine-induced neuropathy is transient and can remit after its withdrawal, the process of complete recovery can be slow. Glutamate carboxypeptidase II (GCP II) inhibition has been shown to improve symptoms of both chemotherapy- and diabetic-induced neuropathy. This study evaluated if GCP II inhibition could behaviorally and physiologically improve pyridoxine-induced neuropathy. In the current study, high doses of pyridoxine (400 mg/kg, twice a day for seven days) were used to induce neuropathy in rats. An orally bioavailable GCP II inhibitor, 2-(3-mercaptopropyl) pentanedioic acid (2-MPPA), was administered daily at a dose of 30 mg/kg starting from the onset of pyridoxine injections. Body weight, motor coordination, heat sensitivity, electromyographical (EMG) parameters and nerve morphological features were monitored. The results show beneficial effects of GCP II inhibition including normalization of hot plate reaction time, foot fault improvements and increased open field distance travelled. H wave frequency, amplitude and latency as well as sensory nerve conduction velocity (SNCV) were also significantly improved by 2-MPPA. Lastly, GCP II inhibition resulted in morphological protection in the spinal cord and sensory fibers in the lumbar region dorsal root ganglia (DRG). In conclusion, inhibition of GCP II may be beneficial against the peripheral sensory neuropathy caused by pyridoxine.

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Section: Discussionsupporting

confidence: 91%

Glutamate Carboxypeptidase II Inhibition Behaviorally and Physiologically Improves Pyridoxine-Induced Neuropathy in Rats

Potter

Wozniak

Callizot³

et al. 2014

PLoS ONE

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“…The plantar muscles were chosen for this study due to their accessibility and the robustness of the H-reflex that can be elicited from this muscle group as described previously (Cliffer, et al, 1998). Furthermore, the stimulation of the tibial nerve at the ankle allows for the plantar muscles to be isolated as much as possible without stimulating other muscle groups, thereby reducing the possibility of reciprocal inhibition or facilitation from other muscle groups (Crone, et al, 2003, Okuma, et al, 2002, Xia and Rymer, 2005.…”

Section: Discussionmentioning

confidence: 99%

“…Chloral hydrate has been shown to produce similar negligible effects on the H-reflex as ketamine (Cliffer, et al, 1998), which is commonly used for H-reflex recordings in rats. Saline (2cc s.c.) was injected approximately 30 minutes before recording to ensure proper hydration of each animal.…”

Section: H-reflex Recordingmentioning

confidence: 99%

Up-regulation of 5-HT2 receptors is involved in the increased H-reflex amplitude after contusive spinal cord injury

Lee

Johnson

Wrathall

2007

Experimental Neurology

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The amplitude of the H-reflex increases chronically after incomplete SCI and is associated with the development of exaggerated hindlimb reflexes. Although the mechanism for this increased H-reflex is not clear, previous studies have shown that pharmacological activation of the 5-HT 2 receptors (5-HT 2 R) can potentiate the monosynaptic reflex. This study tested the hypothesis that increased expression of 5-HT 2 R on motoneurons is involved in increased H-reflex amplitude after a standardized clinically-relevant contusive SCI. Adult female rats were subjected to contusion, complete surgical transection, or a T8 laminectomy only. At 4wks after surgery, H-reflex recordings from the hindpaw plantar muscles of contused rats showed twice the amplitude of that in laminectomy controls or transected rats. To probe the role of 5-HT 2 R in this increased amplitude, dose response studies were done with the selective antagonists, mianserin or LY53857, and the 5-HT 2 R agonist, (±)-1-(2,5-Dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI). The drugs were intrathecally infused into the lumbar cord while recording the H-reflex. Mianserin did not have any significant effects on the H-reflex after transection, consistent with the loss of distal serotonergic innervation. After contusion, both 5-HT 2 R antagonists reduced the H-reflex reflex amplitude with a significantly higher ID 50 compared to the uninjured controls. The 5-HT 2 R agonist, DOI, significantly increased reflex amplitude in contused but not control rats. Furthermore, while 5-HT immunoreactivity was similar, contused rats displayed increased 5-HT 2A R immunoreactivity in plantar muscle motoneurons compared to uninjured controls. We conclude that increased expression of 5-HT 2 R is likely to be involved in the enhanced H-reflex that develops after contusive SCI.

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“…Exposed tissue was covered with mineral oil to prevent drying. A wire electrode was inserted subcutaneously in the digital interosseous muscles between the fourth and fifth metatarsals for EMG recording as previously demonstrated, 26,27 and referenced to a clip applied to the skin on the digits. A ground electrode was attached to the skin of the tail.…”

Section: Reflex Testingmentioning

confidence: 99%

Restoration of frequency-dependent depression of the H-reflex by passive exercise in spinal rats

et al. 2005

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Consistent repeated M- and H-wave recording in the hind limb of rats

Cited by 38 publications

References 33 publications

Glutamate Carboxypeptidase II Inhibition Behaviorally and Physiologically Improves Pyridoxine-Induced Neuropathy in Rats

Glutamate Carboxypeptidase II Inhibition Behaviorally and Physiologically Improves Pyridoxine-Induced Neuropathy in Rats

Up-regulation of 5-HT2 receptors is involved in the increased H-reflex amplitude after contusive spinal cord injury

Restoration of frequency-dependent depression of the H-reflex by passive exercise in spinal rats

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