Constituents of Bamboos and Bamboo Grasses

Tanaka, Nobutoshi; Wada, Hiroshi; Fuchino, Hiroyuki; Inoue, Tatsuo; Yasuda, Daisuke; Kanda, Syuichiro; Kiyokawa, Chiaki; Ashida, Noriaki; Suzuki, Takashi; Yamazaki, Kazuo; Hakamatsuka, Takashi

doi:10.1248/yakushi1947.118.8_332

Cited by 10 publications

(2 citation statements)

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“…In conclusion, this study established a new method for the preparation of tricin from bamboo leaves other than traditional methods such as the chemical synthesis via the Baker−Venkata−Raman reaction between acetylsyringic acid and phloroacetophenone . Previous publications reported that a large amount of tricin or its derivatives is present in the bamboo shoots sheath and in bamboo grasses . At present, tricin was found in bamboo leaves and was obtained from AOB by AB-8 resin column chromatography to aquire a tricin-rich fraction; preparative HPLC was performed for the removal of impurities absorbed under ultraviolet detection with 30% (v/v) acetonitrile in 1% acetic acid as the mobile phase.…”

Section: Resultsmentioning

confidence: 91%

Separation and Purification of Tricin from an Antioxidant Product Derived from Bamboo Leaves

Jiao

Liu

et al. 2007

J. Agric. Food Chem.

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Tricin (5,7,4'-trihydroxy-3',5'-dimethoxyflavone) occurs in its glycosidic form in rice bran and other grass species such as wheat, barley, and maize. Tricin is considered sufficiently safe for clinical development as a cancer chemopreventive agent, therefore it can be used for cancer prevention. This study established a new method for the preparation of tricin from bamboo leaves as an alternative to traditional methods such as chemical synthesis via the Baker-Venkata-Raman reaction between acetylsyringic acid and phloroacetophenone. Tricin was prepared from an antioxidant product that was derived from bamboo leaves (AOB) by extraction with aqueous ethanol. A concentrated solution of this product was obtained and then processed by polystyrene (AB-8) resin column chromatography and preparative high-performance liquid chromatography (HPLC) with 30% (v/v) acetonitrile in 1% (v/v) acetic acid as the mobile phase. The collected tricin-rich fraction was further sequentially purified by dialysis membrane separation and drowning-out crystallization methods. The purity was assessed by analytical HPLC with 25% (v/v) acetonitrile in 1% (v/v) acetic acid as the mobile phase, and the chemical confirmation was evaluated by infrared, mass, nuclear magnetic resonance, and ultraviolet spectroscopies. Tricin (3.09 g) was prepared from 174 g of a crude column chromatography fraction obtained from 5 L of AOB concentrated solution. The present method is appropriate for preparing quantities of pure tricin, which can be used for the quantification of tricin in various plant herbs and further for pharmaceutical/biomedical applications and evaluation.

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Section: Resultsmentioning

confidence: 91%

Separation and Purification of Tricin from an Antioxidant Product Derived from Bamboo Leaves

Jiao

Liu

et al. 2007

J. Agric. Food Chem.

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“…This radical scavenging activity of the SBBF could be related to the nature of antioxidant compounds, thus contributing to their electron transfer/hydrogen donating ability (29). It was reported that the SBBF contained an active compound, isoorientin, which is the nature of flavonoids (30,31). It is supposed that this compound could react as a scavenging or inducing substance of reactive oxygen species.…”

Section: Ros Scavenging Activitymentioning

confidence: 99%

Protective Effect of Sasa borealis Leaf Extract on AAPH-Induced Oxidative Stress in LLC-PK1 Cells

Hwang¹,

Lee²,

Han

2011

JFN

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This study was designed to investigate the protective effect of Sasa borealis leaf extract on 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in LLC-PK1 cells (porcine kidney epithelial cells). The butanol fraction from Sasa borealis leaf extract (SBBF) was used in this study because it possessed strong antioxidant activity and high yield among fractions. Exposure of LLC-PK1 cells to 1 mM AAPH for 24 hr resulted in a significant decrease in cell viability, but SBBF treatment protected LLC-PK1 cells from AAPH-induced cell damage in a dose dependant manner. To determine the protective action of SBBF against AAPH-induced damage of LLC-PK1 cells, we measured the effects of SBBF on lipid peroxidation and antioxidant enzymes activities of AAPH treated cells as well as scavenging activities on superoxide anion radical and hydroxyl radical. SBBF had a protective effect against the AAPH-induced LLC-PK1 cellular damage and decreased lipid peroxidation and increased activities of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase. Furthermore, SBBF showed strong scavenging activity against superoxide anion radical. The IC50 value of SBBF was 28.45±1.28 μg/mL for superoxide anion radical scavenging activity. The SBBF also had high hydroxyl radical scavenging activity (IC50=31.09±3.08 μg/mL). These results indicate that SBBF protects AAPH-induced LLC-PK1 cells damage by inhibiting lipid peroxidation, increasing antioxidant enzyme activities and scavenging free radicals.

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