Constituents of the Essential Oil of Salvia sclarea Growing Wild in Greece

Souleles, Chr.; Argyriadou, N.

doi:10.1076/phbi.35.3.218.13295

Cited by 28 publications

(23 citation statements)

References 2 publications

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“…One clue comes from the observation that NpABC1 expression is strongly enhanced by sclareol, a major diterpene that is toxic for various organisms and that is found in Solanaceae (Bailey et al, 1975), Labiatae (Souleles and Argyriadou, 1997), and Gymnospermae (Pietsch and Konig, 2000) species. It inhibits the growth of rust fungi (Bailey et al, 1975) and various Staphylococcus spp (Demetzos et al, 1999) and induces apoptosis in human leukemia cell lines (Dimas et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

et al. 2001

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ATP binding cassette (ABC) transporters, which are found in all species, are known mainly for their ability to confer drug resistance. To date, most of the ABC transporters characterized in plants have been localized in the vacuolar membrane and are considered to be involved in the intracellular sequestration of cytotoxins. Working on the assumption that certain ABC transporters might be involved in defense metabolite secretion and their expression might be regulated by the concentration of these metabolites, we treated a Nicotiana plumbaginifolia cell culture with sclareolide, a close analog of sclareol, an antifungal diterpene produced at the leaf surface of Nicotiana spp; this resulted in the appearance of a 160-kD plasma membrane protein, which was partially sequenced. The corresponding cDNA ( NpABC1 ) was cloned and shown to encode an ABC transporter. In vitro and in situ immunodetection showed NpABC1 to be localized in the plasma membrane. Under normal conditions, expression was found in the leaf epidermis. In cell culture and in leaf tissues, NpABC1 expression was strongly enhanced by sclareolide and sclareol. In parallel with NpABC1 induction, cells acquired the ability to excrete a labeled synthetic sclareolide derivative. These data suggest that NpABC1 is involved in the secretion of a secondary metabolite that plays a role in plant defense. ń INTRODUCTIONThe ATP binding cassette (ABC) superfamily of membrane transporters is found in all prokaryotic and eukaryotic species. All members of this family are involved in the active transport of many chemically and structurally unrelated compounds and use ATP hydrolysis as a source of energy (Higgins, 1992). Several of these proteins have been identified by their ability to confer drug resistance, hence their designation as multidrug resistance (MDR) or pleiotropic drug resistance (PDR) proteins. For example, the human MDR1 (Chen et al., 1986), which is also designated P-glycoprotein, is implicated in resistance of cell lines to antitumor drugs (Ueda et al., 1987), PDR5 is a multidrug transporter found in Saccharomyces cerevisiae (Balzi et al., 1994), and Candida PDR1 (CDR1) and CDR2, identified in the yeast Candida albicans , contribute to fungicide resistance (Prasad et al., 1995;Sanglard et al., 1997). An analog of human MDR1 also has been found in prokaryotes (van Veen et al., 1996). Other ABC transporters were characterized by their ability to secrete internal compounds; this is the case with the S. cerevisiae protein STE6, which is involved in the transport of a mating pheromone peptide (Kuchler et al., 1989), and mammalian MDR2, which acts as a phospholipid translocator (Ruetz and Gros, 1994).Multidrug resistance-related proteins (MRPs) are ABC proteins that can be distinguished from the proteins described above by their possession of an N-terminal hydrophobic extension and an internal regulatory domain. In mammals, the MRPs HmMRP1 (Cole et al., 1992) and cMOAT (Buchler et al., 1996) transport glutathione S -conjugates and are implicated in the de...

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Section: Discussionmentioning

confidence: 99%

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

et al. 2001

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“…[6][7][8][9][10][11] The effect of the plant extracts on the CNS as well as on skeletal and smooth muscles have also been reported. 12,13 The chemical composition of the essential oil from S. sclarea was found to be almost exclusively determined by the geographical habitat of the plant, growing wild or cultivated, 10,[14][15][16] and few differences were observed from different harvest and cultivation conditions.…”

Section: Introductionmentioning

confidence: 97%

Characterization and enantiomeric distribution of some terpenes in the essential oil of a Uruguayan biotype of Salvia sclarea L.

Lorenzo

Paz

Davies

et al. 2004

Flavour & Fragrance J

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A biotype of clary sage (Salvia sclarea L.) growing in Uruguay was evaluated for its suitability to be cultivated in Uruguay. Essential oils from the inflorescences and leaves of plant harvested at full flowering and early seed ripeness stages were obtained by steam distillation and characterized by GC and GC-MS (quadrupole). Twenty-seven components (91-98% of the total composition) were identified. The oil was characterized by a high content of linalool (8-22%), linalyl acetate (39-48%) and sesquiterpenes, with germacrene D (8-20%) and (E)-β β β β β-caryophyllene (3-5%) as the main components. The enantiomeric distribution of α α α α α-pinene, sabinene, β β β β β-pinene, limonene, linalool, linalyl acetate and germacrene D was studied by multidimensional HRGC-HRGC. The development stage did not influence the oil composition or the enantiomeric distribution of the studied components of the clary sage biotype over the period of evaluation. It is concluded that clary sage can be grown as an economically viable crop in the south of Uruguay.

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“…7A). This ecotype of Salvia sclarea grown in the Western Himalayan region was found to contain higher amounts of monoterpenoid alcohol, linalool, than obtained in the Mediterranean region (Souleles and Argyriadou 1997;Carrubba et al 2002). Percentage of Linalool was found to be 30.50, 41.83 and 46.56 % in Jammu, Srinagar and Leh, respectively (Fig.…”

Section: Antioxidant and Redox Characteristicsmentioning

confidence: 87%

Physio-chemical and antioxidant profiling of Salvia sclarea L. at different climates in north-western Himalayas

Kaur

Bhat

et al. 2015

Acta Physiol Plant

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Salvia sclarea Linn. commonly known as clary sage, is an important medicinal herb with high market demand. To assess properties suitable for commercial exploitation, physiological and biochemical studies were conducted at different climatic zones in the Western Himalayas. These include Jammu (subtropical; 305 m), Srinagar (temperate; 1730 m) and Leh (cold arid; 3505 m) environment. Antioxidant capacity based on radical scavenging and DNA protecting activity of the plants growing at three locations was found to be highest in Srinagar. The cellular damage in terms of lipid peroxidation was found significantly (p B 0.05) higher in Jammu as compared to Srinagar and Leh. SOD and GR showed significant (p B 0.05) differences between all three climatic zones. High expression of GR at higher altitudes is also corroborated by higher reduced state of glutathione. Significant (p B 0.05) increase in floral characteristics like inflorescence and spike length was observed at Leh. Chemical investigation of essential oil revealed the increased percentage of linalool and sclareol, two commercially important constituents, in Leh. 52.9 and 39.4 % increase was observed in the metabolic content of sclareol in Leh as compared to their values in Jammu and Srinagar, respectively. Higher floral biomass and qualitative increase in essential oil suggest that cold arid Himalayan region can be exploited for commercial cultivation of clary sage.

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Constituents of the Essential Oil of Salvia sclarea Growing Wild in Greece

Cited by 28 publications

References 2 publications

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

Characterization and enantiomeric distribution of some terpenes in the essential oil of a Uruguayan biotype of Salvia sclarea L.

Physio-chemical and antioxidant profiling of Salvia sclarea L. at different climates in north-western Himalayas

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