Constituents of the Essential Oils of Garlic and Citronella and Their Vapor-phase Inhibition Mechanism against <i>S.aureus</i>

Abstract

The purpose of this study was to evaluate the vapor-phase antibacterial efficacy of garlic essential oil (GEO) and citronella essential oil (CEO) against four foodborne bacteria and their vapor-phase antibacterial mechanism against Staphylococcus aureus (S.aureus). GC-MS analysis confirmed that diallyl disulfide (29.258 %) and citronellal (36.940 %) were the major constituents of GEO and CEO, respectively. GEO and CEO at concentrations of 80 μL/L could completely inhibit the growth of all tested bacteria and the combinations of GEO with CEO displayed additive effects against the four tested bacteria with fractional inhibitory concentration index (FICI) values of 0.75 or 0.50. Furthermore, the scanning electron microscopy observations, the change of proteins concentration and catalase activity of S.aureus confirmed that the EOs impacting the membrane integrity and catalase activity of S.aureus. Thus, our study will provide a theoretical foundation for the applicability of EOs on the non-contact preservative packaging for food.

Potent Anti-Cutibacterium acnes Activity of the Essential Oils Derived from Allium sativum, Gerdenia jasminoides, and Hedychium coronarium Cultivated in Thailand

“…Previous reports identified the main component, which contrasted with another investigation that found allyl methyl tri-sulfide to be more common than allicin(Ashraf et al 2019). Interestingly, the oil from our study's GC/MS analysis could analyze more compositions than in previous studies(Chekki et al 2014;Dziri et al 2014;Satyal et al 2017;Wang et al 2019;Zhang and Wang 2019). However, the allicin amount of 20.53% was lower than that reported byChekki et al (2014) andWang et al (2019), which was equivalent to those ofZhang and Wang (2019) andDziri et al (2014).…”

“…Interestingly, the oil from our study's GC/MS analysis could analyze more compositions than in previous studies(Chekki et al 2014;Dziri et al 2014;Satyal et al 2017;Wang et al 2019;Zhang and Wang 2019). However, the allicin amount of 20.53% was lower than that reported byChekki et al (2014) andWang et al (2019), which was equivalent to those ofZhang and Wang (2019) andDziri et al (2014). According toBhatwalkar et al (2021), organosulfur compounds derived from garlic were found to have antibacterial action against several Gram-positive bacteria.…”

Biodegradable Alginate Films with ZnO Nanoparticles and Citronella Essential Oil—A Novel Antimicrobial Structure

“…Essential oils and other plant extracts present a huge potential as antibacterial and antioxidant agents and therefore are the subject of many research studies [ 34 , 35 , 36 , 37 ]. The major constituents of citronella essential oil (CEO), among other substances, are citral, citronellal and citronellol [ 38 , 39 ]. CEO is considered a non-toxic biopesticide in the US [ 40 ] which also has a very strong antifungal and antibacterial activity [ 41 , 42 , 43 ].…”

Antibacterial Biodegradable Films Based on Alginate with Silver Nanoparticles and Lemongrass Essential Oil–Innovative Packaging for Cheese

“…Lemongrass essential oil (LGO) is considered a bio-pesticide in the US [48]. The major constituents are citronellal, geraniol, and citronellol [49]. As a hydrophobic extract, the LGO addition to the alginate film will also improve the water vapor permeability (WVP) performance of the polymeric matrix [50][51][52].…”