Constituents of the stem of Cucurbita moschata exhibit antidiabetic activities through multiple mechanisms

Chang, Ching‐Cheng; Hsu, Chih-Ming; Li, Ting-Syuan; Huang, Shen-Da; Lin, Chen-Chen; Yen, Chia‐Hung; Chou, Chang‐Hung; Cheng, Hsueh‐Ling

doi:10.1016/j.jff.2014.06.017

Cited by 28 publications

(16 citation statements)

References 37 publications

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“…In a previous study, a steroid, (22E,24R)-24-methyl-6β-methoxy-5α-cholesta-7,22-diene-3β,5-diol, isolated from Cucurbita moschata (pumpkin), was also shown to possess both insulin sensitizing and insulin substitution functions in insulinresistant FL83B cells in a concentration identical with those of DHM and THC used in this study (Chang et al, 2014), but the mechanisms underlying its functions are not clear. This suggests that other sources may also contain natural compounds with both insulin sensitizing and insulin substitution functions and deserve to be explored.…”

Section: Activation Of the Insulin-signalling Pathway By Dhm And Thc mentioning

confidence: 77%

“…PTP-1B removes the phosphorylation on tyrosine residues of the insulin receptor and the IRSs, resulting in the down-regulation of insulin signalling (Nieto-Vazquez et al, 2007). Therefore, in previous studies, we used TNF-α to induce insulin resistance in FL83B liver cells (Chang et al, 2011(Chang et al, , 2014. The insulin-stimulated glucose uptake and IRS-1 tyrosyl phosphorylation of the cells were obviously suppressed by TNF-α treatment.…”

Section: Introductionmentioning

confidence: 99%

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Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Chang

Chou

Liao

et al. 2015

Journal of Functional Foods

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Section: Activation Of the Insulin-signalling Pathway By Dhm And Thc mentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Chang

Chou

Liao

et al. 2015

Journal of Functional Foods

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“…There are reports on FA for its potential against diabetes and associated complications. It improves insulin sensitivity , hepatic glycogenesis and inhibits gluconeogenesis . It is also found to reduce diabetic induced complications like nephropathy , inflammation, splenotoxicity (stress mediated) .…”

Section: Discussionmentioning

confidence: 99%

High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid

Raj

Swapna

Raghu

2019

Fundamemntal Clinical Pharma

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Oxidative stress and associated complications are the major pathological concerns of diabetic cardiomyopathy (DC). We aim to elucidate the mechanisms by which high glucose (HG) induced alteration in calcium homeostasis and evaluation of the beneficial effect of two concentrations (10 and 25 μm) of ferulic acid (FA). HG was induced in H9c2 cardiomyoblast by treating with glucose (33 mm) for 48 h, and FA was co‐treated. Intracellular calcium ([Ca2+]i) overload was found increased significantly with HG. For elucidation of mechanism, the SERCA pathway and mitochondrial integrity (transmembrane potential and permeability transition pore) were explored. Then, we assessed oxidative stress, and cell injury with brain natriuretic peptide (BNP), atrial natriuretic peptide (ANP), and lactate dehydrogenase (LDH) release. HG caused significant [Ca2+]i overload through downregulation of SERCA2/1, pPLN, and pPKA C‐α; and upregulation of PLN and PKA C‐α and alteration in the integrity of mitochondria with HG. The [Ca2+]i overload in turn caused oxidative stress via generation of reactive oxygen species, lipid peroxidation, and protein carbonylation. This resulted in cell injury which was evident with significant release of BNP, ANP, and LDH. FA co‐treatment was effective to mitigate all pathological changes caused by HG. From the overall results, we conclude that [Ca2+]i overload via SERCA pathway and altered mitochondrial integrity is the main cause for oxidative stress during HG. Based on our result, we report that FA could be an attractive nutraceutical for DC.

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“…Beberapa penelitian pada tikus yang dibuat diabetes menunjukkan ekstrak labu kuning (Cucurbita moschata) memberikan efek hipoglikemik dan bertindak sebagai antidiabetes (Adams et al 2011;Chang et al 2014), ekstrak labu kuning (Cucurbita moschata) dan bubuk bijinya dapat memperbaiki sel islet pankreas dan produksi insulin (Makni et al 2010;Jin et al 2013). Menurut Glew et al (2006) minyak biji labu kuning (Cucurbita moschata) juga mengandung mineral kromium yang diketahui berperan dalam metabolisme karbohidrat, dengan penyerapan kromium terbaik <40 mcg per sajian (Garcia et al 2001).…”

Section: Junita Dkkunclassified

KOMPONEN GIZI, AKTIVITAS ANTIOKSIDAN DAN KARAKTERISTIK SENSORI BUBUK FUNGSIONAL LABU KUNING (Cucurbita moschata) DAN TEMPE

Junita¹,

Setiawan²,

Anwar³

et al. 2017

jgizipangan

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The aim of this research was to determine the nutrient content, antioxidant activity and the sensory characteristics of functional powder from pumpkin (Cucurbita moschata) Keywords: antioxidant activity, functional powder, nutrient content, sensory ABSTRAKPenelitian bertujuan untuk mengetahui kandungan gizi, aktivitas antioksidan, dan karakteristik sensori formula bubuk fungsional berbahan labu kuning serta tempe. Formula yang digunakan terdiri atas 15% bubuk labu kuning, 35% bubuk biji labu, 50% bubuk tempe. Komponen gizi ditentukan berdasarkan dengan analisis proksimat dan spektrofotometri, aktivitas antioksidan metode DPPH (2,2-diphenyl-1-picrylhidrazyl), serta karakteristik sensori formula dianalisis dengan bantuan 39 panelis semi terlatih menggunakan tiga jenis media minuman yaitu air putih, jus jeruk dan susu. Hasil penelitian menunjukkan bahwa bubuk fungsional mengandung 6,84±0,09% kadar air, 3,18±0,02% kadar abu, 35,15±0,07% lemak, 30,15±0,35% protein, 20,85±0,35% karbohidrat, 3,86±0,02% serat kasar, 308,85±0,55 ppm betakaroten, 515,00 ppb kromium, dan 4,59% total gula. Aktivitas antioksidan bubuk sebesar 6765.88 ppm. Karakteristik sensori pada media jus jeruk lebih baik dari media lainnya. Bubuk fungsional ini dapat dimanfaatkan sebagai alternatif dalam manajemen diabetes, namun perlu peningkatan karakter sensori serta telaah interaksi gizi yang lebih mendalam. 1978-1059 EISSN 2407-0920 DOI: 10.25182/jgp.2017.12.2.109.116 Terakreditasi SK Menristek Dikti 12/M/Kp/II/2015 PENDAHULUANPangan fungsional adalah pangan olahan yang mengandung satu atau lebih komponen pangan yang berdasarkan kajian ilmiah mempunyai fungsi fisiologis tertentu diluar fungsi dasarnya, terbukti tidak membahayakan dan bermanfaat bagi kesehatan (BPOM RI 2016). Menurut Kazeem dan Davies (2016), pangan fungsional adalah makanan dan minuman yang mampu mencegah, melindungi dan atau mengobati suatu penyakit dan juga memiliki nilai gizi yang baik.Perkembangan intervensi gizi penyakit yang erat kaitannya dengan jenis makanan, perlu mengikuti perkembangan pangan. Saat ini muncul tren minuman bubuk (powder drink) yang terbuat dari berbagai buah, sayur, dan biji yang mengandung senyawa antioksidan dan protein whey (Suratno et al. 2014).

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Constituents of the stem of Cucurbita moschata exhibit antidiabetic activities through multiple mechanisms

Cited by 28 publications

References 37 publications

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid

KOMPONEN GIZI, AKTIVITAS ANTIOKSIDAN DAN KARAKTERISTIK SENSORI BUBUK FUNGSIONAL LABU KUNING (Cucurbita moschata) DAN TEMPE

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