Constituents of Valeriana glechomifolia Meyer

Salles, Luisa de Andrade; Silva, Andreia L.; Rech, Sandra Beatriz; Zanatta, Nilo; Poser, Gilsane von

doi:10.1016/s0305-1978(99)00124-6

Cited by 25 publications

(17 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…External standards of valepotriates [acevaltrate (ACE), didrovaltrate (DID), and valtrate (VAL)] were isolated from V. glechomifolia and purified by prep-LC using the procedure described by Salles et al (2000). All solvents used for chromatographic purposes were high performance liquid chromatography (HPLC) grade.…”

Section: Methodsmentioning

confidence: 99%

“…V. glechomifolia is capable of accumulating in all of its organs the terpene derivatives valepotriates, and aerial parts of this species are richer in valepotriates than the subterranean organs (Salles et al 2000;Silva et al 2002) in contrast to other valerian-derived phytomedicine sources, which consist primarily of the roots (Houghton 1999). V. glechomifolia has numerous small leaves and roots, displaying a stoloniferous habit and covering areas of soil in elevated areas, particularly in rocky fields.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Maurmann

Rech

Fett-Neto

2008

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Valeriana glechomifolia, a southern Brazilian endemic species commonly known as Valerian, accumulates the bioactive terpene derivatives valepotriates in all of its organs. In vitro growth of V. glechomifolia on solid Murashige and Skoog (MS) without phytohormones at full, 75% (MS 75), or on a modified formulation (M Δ) was compared in stock cultures kept for up to 9 mo. without subculture. Changes in biomass accumulation, development of roots and shoots, and the production of the valepotriates acevaltrate, didrovaltrate, and valtrate were monthly evaluated. The highest biomass accumulation and root development was observed in plants grown on M Δ, whereas better leaf development was detected in M-Δ-and MSmedium-grown plants after 8 and 9 mo. of culture, respectively. Maximal didrovaltrate and valtrate yields were observed in M-Δ-grown plants harvested after 5 and 6 mo. of culture, respectively, whereas acevaltrate concentration was highest on M-Δ-and MS-75-grown plants after 7 mo. of culture. Plants grown for 6 mo. without subculture in M Δ were successfully propagated, showing stable growth and valepotriate yields three-to sixfold higher that those observed in field-grown plants. The results showed a positive effect of combined moderate reduction in salt concentration and increases in selected micronutrients and myo-inositol amounts on both growth and valepotriate yields of extended period stock cultures of V. glechomifolia.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Maurmann

Rech

Fett-Neto

2008

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Detector sensitivity was set to 1.0 Aufs; wavelengths used were 254 nm (dienic valepotriates) and 208 nm (monoenic valepotriate). Valtrate, acevaltrate and didrovaltrate, used as external standards, were isolated from V. glechomifolia as described by Salles et al (2000). The valepotriates (10 mg each) were individually dissolved in methanol, diluted stepwise (0.5, 0.25, 0.125, 0.0625 and 0.03125 mg/ml), and 10 ll were injected into the HPLC in triplicate.…”

Section: Valepotriate Extraction and Analysesmentioning

confidence: 99%

Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

Russowski

Maurmann

Rech

et al. 2006

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A system for growing in liquid medium whole plants of Valeriana glechomifolia, endemic to southern Brazil and capable of accumulating bioactive valepotriates, is described. Murashige and Skoog (MS) and Gamborg B5 (B5) media (1.0·, 0.3· and 0.1· strength) without phytohormones were evaluated after four weeks of culture in relation to growth and valepotriate yield. Plants grown in 1.0· MS displayed greatest growth and valepotriate yields and the study of the light condition showed that plants grown under light and dark had similar weight increase and maximum valepotriate yield, 27.2 mg/g DW and 25.0 mg/g DW, respectively. Valtrate was the most abundant valepotriate, followed by acevaltrate and didrovaltrate.

show abstract

“…), attached to a guard column, NovaPack C 18 Waters 60A (3.920 mm); mobile phase, CH 3 CN/H 2 O, 50:50 (v/v); flow rate, 1 ml min 1 ; detector sensitivity, 1.0 Aufs; detection at 254 nm (dienic valepotriates) and 208 nm (monoenic valepotriate) with retention times of 18.1 min (acevaltrate), 19.8 min (didrovaltrate) and 34.8 min (valtrate). External standards (valtrate, acevaltrate and didrovaltrate) were isolated as described by Salles et al (2000). Valepotriate contents were expressed as a percentage of the freeze-dried weight of the plants.…”

Section: Valepotriate Determinationsmentioning

confidence: 99%

“…In Valeriana glechomifolia Meyer, which is native to southern Brazil, valepotriates have been found in both the aerial and subterraneous parts of the plants (Salles et al 2000). A protocol for in vitro propagation of the species has been established and the valepotriates quantified (Salles et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia

et al. 2004

View full text Add to dashboard Cite

Valeriana glechomifolia is a plant species endemic to southern Brazil that accumulates valepotriates, which are terpene derivatives, in all of its organs. Valepotriates are the presumed sedative generic components of the pharmaceutically used species of Valeriana. The influence of various concentrations of the auxins indole-3-acetic acid, indole-3-butyric acid and alpha-naphthaleneacetic acid on the growth of micropropagated V. glechomifolia was investigated under conditions of transient and continuous exposure. Changes in the development of roots and shoots as well as the production of the valepotriates acevaltrate, valtrate and didrovaltrate (analyzed by high-performance liquid chromatography) were evaluated. The best performance in valepotriate production, growth and survival under ex vitro conditions following plant acclimatization was achieved in the continuous presence of 5.71 microM IAA. When cultured in medium containing IAA plants produced stable levels of valepotriates throughout the entire cultivation period.

show abstract

Constituents of Valeriana glechomifolia Meyer

Cited by 25 publications

References 12 publications

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia

Contact Info

Product

Resources

About