Constituintes químicos, avaliação das atividades citotóxica e antioxidante de Mimosa paraibana Barneby (Mimosaceae)

Nunes, Xirley Pereira; Mesquita, Rafael Ferreira; Silva, Davi A.; Lira, Daysianne Pereira de; Costa, Vicente Carlos O.; Silva, Marianna V. B.; Xavier, Aline Lira; Diniz, Margareth F. F. Melo; Agra, Maria de Fátima

doi:10.1590/s0102-695x2008000500015

Cited by 19 publications

(19 citation statements)

References 24 publications

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“…The (DPPH) stable free radical photocolorimetric method is frequently used to evaluate the antioxidant property of different extracts from plants (Falcão et al, 2006;Vicentino & Menezes, 2007;Nunes et al, 2008). In relation to the antioxidant property of M. salicifolia, the extracts EHF, EBF, EMF and EHT were considered Maytenus salicifolia: triterpenes isolated from stems and antioxidant property of extracts from aerial parts Cássia G. Magalhães et al inactive, but the extracts EMT and EAF showed significant activity at concentrations above 40.0 µg/mL, EAPF and EAT>30.0 µg/mL and EMPF>20.0 µg/mL (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Maytenus salicifolia Reissek, Celastraceae: triterpenes isolated from stems and antioxidant property of extracts from aerial parts

Magalhães¹,

Ferrari²,

Guimarâes³

et al. 2011

Rev. bras. farmacogn.

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Six pentacyclic triterpenes were isolated from hexane extract of stems of Maytenus salicifolia Reissek, Celastraceae: 30-hydroxyfriedelan-3-one (1), 3,16-dioxofriedelane (2), friedeline (3), lupeol (4), betuline (5) and lup-20(29)-en-3,30-diol (6). The structure each one was established on the basis of detailed 1 H and 13 C NMR spectral investigation and by comparison with the respective literature values. For compound 1, the complete 2D NMR (HMBC, HMQC and NOESY) spectral data were herein reported for the first time. Compounds 1, 2, 5 and 6 were isolated for the first time from this plant. Antioxidant activity is described for some extracts from species of the Celastraceae family, then, the extracts from aerial parts of M. salicifolia were evaluated in relation to antioxidant potential using the DPPH method. Compared to quecertin, the AcEt extract (EAF) from leaves, AcEt (EAPF) and MeOH (EMPF) from pulp fruit and AcEt (EAT) and MeOH (EMT) from stems showed significant antioxidant property.

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Section: Resultsmentioning

confidence: 99%

Maytenus salicifolia Reissek, Celastraceae: triterpenes isolated from stems and antioxidant property of extracts from aerial parts

Magalhães¹,

Ferrari²,

Guimarâes³

et al. 2011

Rev. bras. farmacogn.

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“…De acordo com a literatura, estudos mostram que tanto óleos essenciais (Souza et al, 2007) como componentes não voláteis (extratos), extraídos de plantas (Balestrin et al, 2008, Nunes et al, 2008, têm sido estudados quanto à a avaliação do seu potencial antioxidante, demonstrando alta eficiência.…”

Section: Introductionunclassified

Metabólitos Secundários e ação antioxidante de Croton helliotripifolius e Croton blanchetianus

et al. 2017

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ResumoAntioxidantes são compostos que reagem com os radicais livres controlando o estresse oxidativo. Muitas doenças estão associadas à produção de radicais livres, os quais tem estimulado investigações do potencial antioxidante de diversas famílias da flora mundial. Essa pesquisa teve como objetivo identificar as principais classes de metabólitos secundários presentes nos estratos etanólicos das folhas de Croton blanchetianus (Marmeleiro) e Croton heliotropiifolius (Velame), e avaliar o seu potencial antioxidante. Os estratos etanólicos foram obtidos das folhas, apresentou um rendimento de 3,29% e 3,26%. Em seguida, os estratos foram submetidos a análise fitoquímica, nos quais foi possível identificar a presença de taninos condensados, flavonoides, flavononas, flavonóis, flavononóis, catequinas e xantonas. A atividade antioxidante dos estratos foi determinada pelo método fotocolorimétrico in vitro realizada por meio do sequestro de radicais livres, usando o DPPH (1,1-difenil-2-picrilhidraliza). Ambos os estratos foram capazes de sequestrar o radical livre DPPH, sendo que o estrato de Croton blanquetianus mostrou ser mais eficiente do que o BHT (Hidroxitolueno butilado; controle positivo), com CE50 = 6,5 ± 0,5 mg mL -1 . Contudo, os resultados observados para os estratos etanólicos, possivelmente sejam atribuídas à presença e a concentração de compostos fenólicos pertencentes as classes dos taninos e flavonoides, os quais foram identificados nesse estudo. Palavras-chave: Radicais livres, fitoquímica, fotocolorimétrico Secondary metabolites and antioxidant action of Croton heliotropiifolius andCroton blanchetianus Abstract Antioxidants are compounds that, at optimal concentrations in relation to oxidable substrates, react with free radicals preventing or reducing oxidative stress. Many diseases and degenerative processes are associated with overproduction of free radicals. This stimulated several research groups to investigate the antioxidant potential of substances produced by several families of the world flora. This study identified the major classes of secondary metabolites present in ethanol leaf extracts of C. blanchetianus (Marmeleiro) and C. heliotropiifolius (Velame), and assessed their antioxidant potential. Ethanol extracts were obtained by the exhaustive extraction method cold and resulted in 3.29% and 3.26% extraction efficiency. Then phytochemical analyses were performed on the extracts to investigate the presence of tannins, flavonoids, flavonones, flavonols, catechins and xanthones. The antioxidant activity of extracts was determined by in vitro photocolorimetric method based on the sequestration of free radicals, using DPPH (1,1-diphenyl-2-picryl-hydrazyl). Both extracts were able to sequestrate DPPH free radical. Croton blanchetianus extract activity showed to be more efficient than BHT (Butylated Hidroxytoluene) (positive control), with EC50 = 6.5 ± 0.5 mg mL -1 . Croton heliotropiifolius extracts showed lower antioxidant activity, with EC50 = 50.15 ± 0.21 but still higher than that observed fo...

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“…The brine shrimp lethality bioassay (BSLB) has been used as an efficient, rapid and inexpensive front-line test for the detection of bioactive compounds (Meyer et al, 1982;Ghisalberti, 1997;Lhullier et al, 2006;Stefanello et al, 2006;Silva et al, 2007;Nunes et al, 2008;Shoeb et al, 2008;Subhan et al, 2008). This bioassay requires small amount of sample (Solis et al, 1993), and it generally correlates well with cytotoxic (McLaughlin et al, 1998) and trypanosomicidal (Zani et al, 1995) activities.…”

Section: Cytotoxicity Of Wedelia Paludosa DC Extracts and Constituentsmentioning

confidence: 99%

Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

Batista¹,

Brandão²,

Braga³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: "Citotoxidade de extratos e constituintes de Wedelia paludosa D.C." Wedelia paludosa D.C. (Asteraceae) é uma planta ornamental facilmente encontrada em várias regiões do Brasil, principalmente nos estados de Santa Catarina, São Paulo, Minas Gerais, Bahia e Pernambuco. Objetivando descobrir novas substâncias citotóxicas a partir desta espécie, o extrato hidrometanólico de W. paludosa (HME) e as frações diclorometânica (FD) e aquosa (FA) resultantes de sua partição em CH 2 Cl 2 -H 2 O foram avaliados utilizando-se o bioensaio em Artemia salina. A fração diclorometânica (FD) apresentou a maior atividade citotóxica (CL 50 = 140,6 mg/mL), e sua análise por cromatografia líquida de alta eficiência empregando-se fase reversa (FR-CLAE) revelou os ácidos caurenóico (1, 6,22 ± 0,23%) e grandiflorênico (2, 3,22 ± 0,31%) como constituintes majoritários. As amostras HME (CL 50 = 980 mg/mL), FD (CLC 50 = 140,6 mg/mL), 1 (CL 50 = 15,9 mg/mL) e 2 (CL 50 = 29,8 mg/mL) foram citotóxicas contra A. salina, enquanto que a fração aquosa (FA, CL 50 >> 1000 mg/mL) mostrou-se inativa. Conclui-se que a citotoxidade observada para HME e FD pode ser atribuída à presença dos ácidos caurenóico (1) e grandiflorênico (2) nestes extratos.Unitermos: Wedelia paludosa, Asteraceae, atividade citotóxica, toxidade em Artemia salina, ácido caurenóico, ácido grandiflorênico, CLAE.ABSTRACT: Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC 50 = 140.6 mg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC 50 = 980 mg/mL), DF (LC 50 = 140.6 mg/mL), 1 (LC 50 = 15.9 mg/ mL) and 2 (LC 50 = 29.8 mg/mL) were found to be cytotoxic, while the water fraction (WF, LC 50 >> 1000 mg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.

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Constituintes químicos, avaliação das atividades citotóxica e antioxidante de Mimosa paraibana Barneby (Mimosaceae)

Cited by 19 publications

References 24 publications

Maytenus salicifolia Reissek, Celastraceae: triterpenes isolated from stems and antioxidant property of extracts from aerial parts

Maytenus salicifolia Reissek, Celastraceae: triterpenes isolated from stems and antioxidant property of extracts from aerial parts

Metabólitos Secundários e ação antioxidante de Croton helliotripifolius e Croton blanchetianus

Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

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