Constitutive and elicitation induced metabolism of isoflavones and pterocarpans in chickpea (Cicer arietinum) cell suspension cultures

Barz, Wolfgang; Mackenbrock, Ulrike

doi:10.1007/978-94-011-0237-7_14

Cited by 13 publications

(14 citation statements)

References 24 publications

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“…a,b Absorbance at 270 nm; c-f absorbance at 287 nm to UV light and fungal elicitor (Dangl et al 1987), with elicitor inducing furanocoumarin phytoalexins and UV light inducing flavonoid glycosides. In legumes such as alfalfa, soybean and chickpea, the major response to yeast or fungal elicitors is accumulation of isoflavonoid phytoalexins and related compounds (Barz and Mackenbrock 1994;Dixon 1999). In the M. truncatula cultures, the fold-induction of soluble isoflavonoids (medicarpin and its conjugate) in response to YE was significantly lower than previously reported in alfalfa cultures exposed to Colletotrichum elicitor (Dalkin et al 1990), in spite of the high fold-induction of PAL and CHS transcripts.…”

Section: Changes In Triterpenoids In Response To Meja and Yecontrasting

confidence: 71%

“…Elicitor-mediated accumulation of phenylpropanoid compounds in plant cell cultures has been studied in greatest detail in parsley, chickpea, soybean and alfalfa cells (Kombrink and Hahlbrock, 1986;Barz and Mackenbrock 1994;Dixon 1999). Parsley cell cultures and protoplasts derived from them respond differently Identifications of compounds were based on comparison of UV spectra (from diode array detection) and retention times to those of authentic standards.…”

Section: Changes In Triterpenoids In Response To Meja and Yementioning

confidence: 99%

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Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula

Suzuki

Reddy

Naoumkina

et al. 2004

Planta

175

139

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Exposure of cell suspension cultures of Medicago truncatula Gaerth. to methyl jasmonate (MeJA) resulted in up to 50-fold induction of transcripts encoding the key triterpene biosynthetic enzyme beta-amyrin synthase (betaAS; EC 5.4.99.-). Transcripts reached maximum levels at 24 h post-elicitation with 0.5 mM MeJA. The entry point enzymes into the phenylpropanoid and flavonoid pathways, L: -phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and chalcone synthase (CHS; EC 2.3.1.74), respectively, were not induced by MeJA. In contrast, exposure of cells to yeast elicitor (YE) resulted in up to 45- and 14-fold induction of PAL and CHS transcripts, respectively, at only 2 h post-elicitation. betaAS transcripts were weakly induced at 12 h after exposure to YE. Over 30 different triterpene saponins were identified in the cultures, many of which were strongly induced by MeJA, but not by YE. In contrast, cinnamic acids, benzoic acids and isoflavone-derived compounds accumulated following exposure of cultures to YE, but few changes in phenylpropanoid levels were observed in response to MeJA. DNA microarray analysis confirmed the strong differential transcriptional re-programming of the cell cultures for multiple genes in the phenylpropanoid and triterpene pathways in response to MeJA and YE, and indicated different responses of individual members of gene families. This work establishes Medicago cell cultures as an excellent model for future genomics approaches to understand the regulation of legume secondary metabolism.

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Section: Changes In Triterpenoids In Response To Meja and Yecontrasting

confidence: 71%

Section: Changes In Triterpenoids In Response To Meja and Yementioning

confidence: 99%

Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula

Suzuki

Reddy

Naoumkina

et al. 2004

Planta

175

139

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“…On the physiological level, elicitation of cultured cells stimulated a signal transduction pathway leading to several rapid responses including an oxidative burst, extracellular alkalinisation followed by extracellular acidification, transient K+ efflux, and activation of defence-related genes, all within 2 h. Rapidly and transiently expressed genes encoded the first soluble enzyme in the pterocarpan biosynthesis part of the medicarpin and maackiain malonylglucoside phytoalexin pathway (Mackenbrock et al, 1993), a NADPH: isoflavone oxidoreductase (IFR; Tiemann et al, 1991), and at least eight members of the cytochrome P450 protein family also involved in isoflavone synthesis. Thus, it seems that isoflavone metabolism is of considerable importance for resistance to A. rabiei (Barz & Mackenbrock, 1994;Overkamp et al, 2000). Increased expression was also found for mRNAs of rab and rac type small GTPbinding proteins (Ichinose et al, 1999), and for genes encoding two glycine-rich proteins (GRPs), which displayed maximum expression 5 days post infection and are probably involved in fortification of cell walls by oxidative cross-linking of cell wall components .…”

Section: Infection Of Chickpea By Ascochyta Rabiei: the Transcriptomementioning

confidence: 92%

Chickpea molecular breeding: New tools and concepts

et al. 2006

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Chickpea is a cool season grain legume of exceptionally high nutritive value and most versatile food use. It is mostly grown under rain fed conditions in arid and semi-arid areas around the world. Despite growing demand and high yield potential, chickpea yield is unstable and productivity is stagnant at unacceptably low levels. Major yield increases could be achieved by development and use of cultivars that resist/tolerate abiotic and biotic stresses. In recent years the wide use of early maturing cultivars that escape drought stress led to significant increases in chickpea productivity. In the Mediterranean region, yield could be increased by shifting the sowing date from spring to winter. However, this is hampered by the sensitivity of the crop to low temperatures and the fungal pathogen Ascochyta rabiei. Drought, pod borer (Helicoverpa spp.) and the fungus Fusarium oxysporum additionally reduce harvests there and in other parts of the world. Tolerance to rising salinity will be a future advantage in many regions. Therefore, chickpea breeding focuses on increasing yield by pyramiding genes for resistance/tolerance to the fungi, to pod borer, salinity, cold and drought into elite germplasm. Progress in breeding necessitates a better understanding of the genetics underlying these traits. Marker-assisted selection (MAS) would allow a better targeting of the desired genes. Genetic mapping in chickpea, for a long time hampered by the little variability in chickpea's genome, is today facilitated by highly polymorphic, co-dominant microsatellite-based markers. Their application for the genetic mapping of traits led to inter-laboratory comparable maps. This paper reviews the current situation of chickpea genome mapping, tagging of genes for ascochyta blight, fusarium wilt resistance and other traits, and requirements for MAS. Conventional breeding strategies to tolerate/avoid drought and chilling effects at flowering time, essential for changing from spring to winter sowing, are described. Recent approaches and future prospects for functional genomics of chickpea are discussed. Chickpea: A valuable grain legumeChickpea (Cicer arietinum L.) is the only cultivated species within the genus Cicer. The crop is a selfpollinated diploid (2n = 2x = 16) with a relatively small genome (740 Mb, Arumuganathan & Earle, 1991). It is cultivated in arid and semi-arid areas around the world. With over 10 million ha under cultivation, chickpea is second only to common bean (Phaseolus vulgaris) and third in production among the legumes. The major producers India, Pakistan and Turkey contribute 65%, 9.5% and 6.7% respectively,

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“…Elicitation particularly induces plant cells to synthesize phytoalexins at elevated levels. In regard to this, possibility of enhanced accumulation of isoflavones by elicitation has been reported in cell suspension cultures [17,23,24]. However, there are only few reports of application of elicitors to hairy root cultures [25,26].…”

Section: Introductionmentioning

confidence: 95%

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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^Äëíê~Åí= The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine and spermidine) was studied with respect to isoflavones accumulation in hairy root cultures of mëçê~äÉ~=ÅçêóäáÑçäá~=L. Untreated hairy roots (control) accumulated 1.55% dry wt of daidzein and 0.19% dry wt of genistein. In precursor feeding experiment, phenylalanine at 2 mM concentration led to 1.3 fold higher production of daidzein (1.91% dry wt) and genistein (0.27% dry wt). In biotic elicitors, chitosan (2 mg/L) was found to be the most efficient elicitor to induce daidzein (2.78% dry wt) and genistein (0.279% dry wt) levels in hairy roots. Salicylic acid at 1 mM concentration stimulated the maximum accumulation of daidzein (2.2% dry wt) and genistein (0.228% dry wt) 2 days after elicitation. In case of polyamines, putrescine (50 mM) resulted in highest accumulation of daidzein (3.01% dry wt) and genistein (0.227% dry wt) after 5 days of addition. Present results indicated the effectiveness of elicitation and precursor feeding on isoflavones accumulation in hairy roots of mK=ÅçêóäáÑçäá~. This is the first report of elicitation on isoflavones production by hairy roots of mK=ÅçêóäáÑçäá~. © KSBB hÉóïçêÇëW=Psoralea corylifoliaI=éÜóíçÉëíêçÖÉåëI=ÉäáÅáí~íáçåI=éêÉÅìêëçêI=ëáÖå~äáåÖ=ãçäÉÅìäÉ

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Constitutive and elicitation induced metabolism of isoflavones and pterocarpans in chickpea (Cicer arietinum) cell suspension cultures

Cited by 13 publications

References 24 publications

Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula

Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula

Chickpea molecular breeding: New tools and concepts

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

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