2014
DOI: 10.1074/jbc.m113.516740
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Constitutive and Nitrogen Catabolite Repression-sensitive Production of Gat1 Isoforms

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Cited by 11 publications
(30 citation statements)
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References 62 publications
(106 reference statements)
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“…S5). Together with the observation that altering ATG M1 did not lead to a detectable phenotype, 44 the lack of overall conservation of the full-length open reading frame among S. cerevisiae S288C's closest relatives suggested that the 1st in-phase methionine in the S288C sequence of Gat1 did not function in vivo under the conditions we used in our experiments.…”
Section: Resultsmentioning
confidence: 69%
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“…S5). Together with the observation that altering ATG M1 did not lead to a detectable phenotype, 44 the lack of overall conservation of the full-length open reading frame among S. cerevisiae S288C's closest relatives suggested that the 1st in-phase methionine in the S288C sequence of Gat1 did not function in vivo under the conditions we used in our experiments.…”
Section: Resultsmentioning
confidence: 69%
“…Interestingly, neither of these isoforms required the first in-frame methionine for their production. 44 Indeed, using amino acid substitutions, we showed that translation of Gat1 is initiated at M40 and M95 relative to the beginning of the open reading frame. 44 Phylogenetic analysis was carried out by comparing the amino acid sequences deduced from the open reading frames of Gat1 orthologs from 25 S. cerevisiae strains available on Saccharomyces Genome Database ( Fig.…”
Section: Resultsmentioning
confidence: 93%
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