Constitutive and Secretory Expression of the AiiA in &amp;lt;i&amp;gt;Pichia pastoris&amp;lt;/i&amp;gt; Inhibits &amp;lt;i&amp;gt;Amorphophallus konjac&amp;lt;/i&amp;gt; Soft Rot Disease

Wu, Jiang; Jiao, Zhenbiao; Guo, Fengling; Chen, Leifu; Ding, Zhong‐Tao; Qiu, Zhengming

doi:10.4236/ajmb.2016.62009

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…The purified AiiA showed efficient inhibition to soft rot of Amorphophallus konjac by targeting the QS system of E. carotovora Wu et al (2016) The rotten rate of potato was reduced by 92.85% by AiiA Suryanti et al (2022) The rotted area of potato was reduced by 37.4% by Pseudomonas putida cells containing AiiA Li (2011) Acylase LPC029…”

Section: Fruits and Vegetablesmentioning

confidence: 99%

“…These pathogens threaten food production worldwide, and QQ enzymes offer an attractive approach to counteract the significant loss of quantity and quality of fruits and vegetables caused by plant pathogens. As shown in Table 2, there are many reports of QQ enzymes inhibiting quality deterioration of different fruits and vegetables such as pear, potato, carrot, eggplant, and Chinese cabbage (Chankhamhaengdecha et al., 2013; Li, 2011; Suryanti et al., 2022; Wu et al., 2016). MomL is a novel AHL lactonase from Muricauda olearia Th120 with broad‐spectrum AHL hydrolysis activity.…”

Section: Application Of Qq Enzymes In the Food Industrymentioning

confidence: 99%

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Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Dangfeng

Cui

Ren

et al. 2023

Comp Rev Food Sci Food Safe

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The problems of spoilage, disease, and biofilm caused by bacterial quorum‐sensing (QS) systems have posed a significant challenge to the development of the food industry. Quorum‐quenching (QQ) enzymes can block QS by hydrolyzing or modifying the signal molecule, making these enzymes promising new candidates for use as antimicrobials. With many recent studies of QQ enzymes and their potential to target foodborne bacteria, an updated and systematic review is necessary. Thus, the goals of this review were to summarize what is known about the effects of bacterial QS on the food industry; discuss the current understanding of the catalytic mechanisms of QQ enzymes, including lactonase, acylase, and oxidoreductase; and describe strategies for the engineering and evolution of QQ enzymes for practical use. In particular, this review focuses on the latest progress in the application of QQ enzymes in the field of food. Finally, the current challenges limiting the systematic application of QQ enzymes in the food industry are discussed to help guide the future development of these important enzymes.

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Section: Fruits and Vegetablesmentioning

confidence: 99%

Section: Application Of Qq Enzymes In the Food Industrymentioning

confidence: 99%

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Dangfeng

Cui

Ren

et al. 2023

Comp Rev Food Sci Food Safe

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“…( Wu et al, 2012 ), Dicyeya spp. ( Czajkowski et al, 2014 ; Wu et al, 2016 ), and Enterobacter spp. ( Wu et al, 2011b ).…”

Section: Introductionmentioning

confidence: 99%

Response of Root-Associated Bacterial Communities to Different Degrees of Soft Rot Damage in Amorphophallus konjac Under a Robinia pseudoacacia Plantation

2021

Front. Microbiol.

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Bacterial soft rot is a destructive disease that restricts the development of the konjac (Amorphophallus konjac K. Koch ex N.E.Br) industry. The objective of this study was to investigate how soft rot disease affects bacterial communities associated with the roots of konjac plants growing under a pure Robinia pseudoacacia plantation. Three sampling sites affected by different degrees of soft rot damage were selected based on the disease incidence [0%, non-diseased (ND); 4.2%, moderately diseased (MD); and 18.6%, highly diseased (HD)]. The variation in soil and root bacterial diversity and community composition among the sampling sites was determined by Illumina HiSeq sequencing of the V3–V4 hypervariable regions of the bacterial 16S rRNA gene. The results showed that the contents of soil organic matter and available nutrients (N, P, and K) increased with increasing damage degree, whereas higher damage degree resulted in lower soil pH and enzymatic activity (sucrase, urease, catalase, and polyphenol oxidase). The composition of root-associated bacterial communities differed among the three sampling sites. Proteobacteria was the most dominant bacterial phylum in all soil and root samples. Pseudomonas, Bacillus, Rhizobium, and Streptomyces were the most abundant in all samples from the ND sites, whereas Pectobacterium carotovorum and Serratia were predominant in the samples from the MD and HD sites. The abundance and alpha diversity of root-associated bacteria were significantly higher (p < 0.05) in the ND sites than in the diseased sites. The results suggested pronounced differences in the abundance, alpha diversity, and community composition of bacteria associated with the roots of konjac plants affected by different degrees of soft rot damage. Such differences in bacterial community structure were related to dynamic changes in soil variables, especially soil available potassium content, sucrase activity, and urease activity. Analysis of the dominant root-associated bacterial taxa offers an approach to predict the damage degree due to soft rot in konjac and provides evidence for the prevention of this soil-borne disease via microecological regulation.

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“…Soft rot disease is a devastating soilborne disease caused by Pectobacterium, Dicyeya, and Enterobacter (Charkowski 2018;Wei et al 2020;Wu et al 2016). The typical symptoms of Amorphophallus infection with the soft rot pathogen are the production of water stains at the initial stage, followed by the tissue becoming soft and rotten and emitting a foul odor.…”

mentioning

confidence: 99%

Weighted Gene Coexpression Network Analysis of Candidate Pathways and Genes in Soft Rot Resistance of Amorphophallus

Gao

Wei

Liu

et al. 2022

J. Amer. Soc. Hort. Sci.

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Amorphophallus species are one of the main economic crops in the mountainous areas of southwest China. However, soft rot disease (Pectobacterium carotovorum ssp. carotovorum) is devastating for this crop. This study explored the Amorphophallus resistance mechanism against soft rot disease by analyzing transcriptome data using a weighted gene coexpression network analysis. The RNA sequencing of plants infected for 0, 12, 24, and 48 hours produced a total of 52.25 Gb of clean reads. A total of 29,096 genes were divided into 34 modules. Six modules of interest with the highest correlation with the target traits were selected to elucidate the resistance genes and pathways. The selected modules were enriched in the α-linolenic acid metabolism, phenylpropane biosynthesis, plant hormone signal transduction, and plant pathogen interaction pathways. Ultimately, AmBGLU, AmCAML, AmCDPK, AmLOX, and AmRBOHD were identified as genes of interest in the four significantly related metabolic pathways for real-time fluorescence quantitative polymerase chain reaction verification. The determination of salicylic acid (SA) and jasmonic acid (JA) in Amorphophallus muelleri and Amorphophallus konjac that suffered from soft rot disease showed that SA and JA were involved in the A. muelleri and A. konjac defense response against soft rot disease. Methyl jasmonate treatment delayed the onset of A. konjac soft rot disease. This study provides a reference for the interaction between Amorphophallus species and soft rot disease and the breeding of broad-spectrum and specific Amorphophallus cultivars that are resistant to soft rot disease.

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Constitutive and Secretory Expression of the AiiA in <i>Pichia pastoris</i> Inhibits <i>Amorphophallus konjac</i> Soft Rot Disease

Cited by 9 publications

References 23 publications

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Response of Root-Associated Bacterial Communities to Different Degrees of Soft Rot Damage in Amorphophallus konjac Under a Robinia pseudoacacia Plantation

Weighted Gene Coexpression Network Analysis of Candidate Pathways and Genes in Soft Rot Resistance of Amorphophallus

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