Constitutive auxin response in Physcomitrella reveals complex interactions between Aux/IAA and ARF proteins

Lavy, Meirav; Prigge, Michael J.; Tao, Sibo; Shain, Stephanie; Kuo, April; Kirchsteiger, Kerstin; Estelle, Mark

doi:10.7554/elife.13325

Cited by 135 publications

(167 citation statements)

References 62 publications

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“…Alternatively, it is possible that Aux/IAA target genes that are not directly associated with growth are involved in stress tolerance, since many auxin responsive genes are not obviously related to growth [33]. In fact, a recent study in the moss Physcomitrella patens demonstrates that over a third of the genes in genome are regulated by the Aux/IAA proteins [34]. …”

Section: Discussionmentioning

confidence: 99%

Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors

et al. 2017

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Summary The Aux/IAA proteins are auxin-sensitive repressors that mediate diverse physiological and developmental processes in plants [1, 2]. There are 29 Aux/IAA genes in Arabidopsis that exhibit unique but partially overlapping patterns of expression [3] (Figure S1A). Although some studies have suggested that individual Aux/IAA genes have specialized function, genetic analyses of the family have been limited by the scarcity of loss-of-function phenotypes [4]. Further, with a few exceptions, our knowledge of the factors that regulate Aux/IAA expression is limited [1, 5]. We hypothesize that transcriptional control of Aux/IAA genes plays a central role in the establishment of the auxin-signaling pathways that regulate organogenesis, growth, and environmental response. Here we describe a screen for transcription factors (TFs) that regulate the Aux/IAA genes. We identify TFs from 38 families including 26 members of the DREB/CBF family. Several DREB/CBF TFs directly promote transcription of the IAA5 and IAA19 genes in response to abiotic stress. Recessive mutations in these IAA genes result in decreased tolerance to stress conditions demonstrating a role for auxin in abiotic stress. Our results demonstrate that stress pathways interact with the auxin gene regulatory network (GRN) through transcription of the Aux/IAA genes. We propose that the Aux/IAA genes function as hubs that integrate genetic and environmental information to achieve the appropriate developmental or physiological outcome.

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Section: Discussionmentioning

confidence: 99%

Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors

et al. 2017

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“…) and the double mutant Dr5:DsRed2 (Lavy et al . ) were routinely cultivated as previously described (Hiss et al . ) on solidified (0.7% [w/v] agar) mineral medium, also known as modified (Reski & Abel ) Knop's medium (Knop ), on 9‐cm Petri dishes sealed using 3M Micropore tape or Parafilm.…”

Section: Methodsmentioning

confidence: 99%

“…; Lavy et al . ). Albeit this mutant was not able to self, multiple crossed sporophytes were detected when paired with Re.…”

Section: Introductionmentioning

confidence: 97%

Physcomitrella patens Reute mCherry as a tool for efficient crossing within and between ecotypes

2018

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Physcomitrella patens is a monoecious moss that is predominantly selfing in the wild. Laboratory crossing techniques have been established and crosses between the sequenced Gransden ecotype and the genetically divergent Villersexel ecotype were used for genetic mapping. The recently introduced ecotype Reute has a high fertility rate and is genetically more closely related to the Gransden ecotype than the Villersexel ecotype. Reute sexual reproduction phenology is similar to Gransden, which should allow successful crossing. Using the Reute ecotype and an existing Gransden mutant as a test case, we applied a normalised crossing approach to demonstrate crossing potential between these ecotypes. Also, using a standard transformation approach, we generated Reute fluorescent strains expressing mCherry that allow an easy detection of crossed offspring (sporophyte). We show that Reute can be successfully crossed with a self-infertile DR5:DsRed2 mutant generated in the Gransden background. Using newly established Reute fluorescent strains, we show that they can efficiently fertilise Reute as well as Gransden wild type. The resulting progeny display Mendelian 1:1 segregation of the fluorescent marker(s), demonstrating the suitability of such strains for genetic crossing. Overall our results demonstrate that Reute is highly suitable for genetic crossing. The Reute mCherry strain can be used as a suitable background for offspring selection after crossing.

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“…Takayuki Kohchi (Kyoto University, Kyoto, Japan) discussed the fact that auxin is required throughout the life cycle of Marchantia, which has the simplest set of auxin signaling components identified so far, as illustrated by the phenotypes observed in inducible lines expressing an auxin-insensitive MpAux/IAA (Kato et al, 2015). As in Physcomitrella (Lavy et al, 2016), these phenotypes suggest a role for auxin in growth, cell division, meristem activity and reproduction that has been conserved up to the angiosperms. Hirotoka Kato from Dolf Weijers' laboratory (University of Wageningen, Wageningen, The Netherlands) analyzed functional divergence between the three ARFs of Marchantia.…”

Section: Auxin Through Plant Evolutionmentioning

confidence: 99%

“…Thus, while spacing specificity is observed, it might not be the only parameter explaining the binding of ARFs to specific promoters. The fact that the same promoters are targeted by both activating and repressing ARFs appears to be an ancestral property that is already observed in the moss Physcomitrella patens, based on evidence presented by Mark Estelle (Lavy et al, 2016).…”

Section: Specificity Of Arf-dependent Regulationmentioning

confidence: 99%

Auxin 2016: a burst of auxin in the warm south of China

Vernoux

Robert

2017

Development

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The luxurious vegetation at Sanya, the most southern location in China on the island of Hainan, provided a perfect environment for the 'Auxin 2016' meeting in October. As we review here, participants from all around the world discussed the latest advances in auxin transport, metabolism and signaling pathways, highlighting how auxin acts during plant development and in response to the environment in combination with other hormones. The meeting also provided a rich perspective on the evolution of the role of auxin, from algae to higher plants.

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Constitutive auxin response in Physcomitrella reveals complex interactions between Aux/IAA and ARF proteins

Cited by 135 publications

References 62 publications

Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors

Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors

Physcomitrella patens Reute mCherry as a tool for efficient crossing within and between ecotypes

Auxin 2016: a burst of auxin in the warm south of China

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