Constitutive Expression of Pluripotency-Associated Genes in Mesodermal Progenitor Cells (MPCs)

Pacini, Stefania; Carnicelli, Vittoria; Trombi, Luisa; Montali, Marina; Fazzi, Rita; Lazzarini, Edoardo; Giannotti, Stefano; Petrini, Mario

doi:10.1371/journal.pone.0009861

Cited by 47 publications

(40 citation statements)

References 33 publications

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“…Moreover, MPCs showed expression of pluripotency-associated markers and Nestin, all rapidly lost during mesengenic differentiation 7 . Sprouting assay revealed MPC ability to invade murine ECM protein gel.…”

Section: Representative Resultsmentioning

confidence: 99%

“…Nonetheless, the initial enthusiasm about mesodermal differentiation potential of MPCs is actually waning. In fact, after more than 7 years of studies on MPCs, mesengenic and angiogenic potential have been extensively described [5][6][7][8][9] , but differentiation toward any other cells of mesodermal origin is still lacking. Thus, here we propose a new, and more rigorous, definition of these cells as "Mesangiogenic Progenitor Cells", maintaining the acronym MPCs.…”

Section: Representative Resultsmentioning

confidence: 99%

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Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Montali

Barachini

Pacini

et al. 2016

JoVE

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In a research study aimed to isolate human bone marrow (hBM)-derived Mesenchymal Stromal Cells (MSCs) for clinical applications, we identified a novel cell population specifically selected for growth in human serum supplemented medium. These cells are characterized by morphological, phenotypic, and molecular features distinct from MSCs and we named them Mesodermal Progenitor Cells (MPCs). MPCs are round, with a thick highly refringent core region; they show strong, trypsin resistant adherence to plastic. Failure to expand MPCs directly revealed that they are slow in cycling. This is as also suggested by Ki-67 negativity. On the other hand, culturing MPCs in standard medium designed for MSC expansion, gave rise to a population of exponentially growing MSC-like cells. Besides showing mesenchymal differentiation capacity MPCs retained angiogenic potential, confirming their multiple lineage progenitor nature. Here we describe an optimized highly reproducible protocol to isolate and characterize hBM-MPCs by flow cytometry (CD73, CD90, CD31, and CD45), nestin expression, and F-actin organization. Protocols for mesengenic and angiogenic differentiation of MPCs are also provided. Here we also suggest a more appropriate nomenclature for these cells, which has been re-named as "Mesangiogenic Progenitor Cells".

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Section: Representative Resultsmentioning

confidence: 99%

Section: Representative Resultsmentioning

confidence: 99%

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Montali

Barachini

Pacini

et al. 2016

JoVE

Self Cite

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“…Sall4 is expressed in extraembryonic endoderm cells, which are stem cells that do not express Oct4 (Lim et al, 2008), fetal liver stem/progenitor cells but not adult liver stem cells (Oikawa et al, 2009), neural crest stem cells (Barembaum and BronnerFraser, 2010), mesoderm progenitor cells (Pacini et al, 2010), hematopoietic stem cells (Yang et al, 2007), endometrium cells (Forte et al, 2009), parathyroid gland stem cells (Shih et al, 2009), and multipotent adult progenitor cells (Ulloa-Montoya et al, 2007), but is not expressed in fully differentiated cells. As expected, Sall4 is expressed in cells of adult mouse ovaries and testes (Kohlhase et al, 2002).…”

Section: Expression Pattern Of Sall4 During Epimorphic Limb Regenerationmentioning

confidence: 99%

Dedifferentiation and the role of sall4 in reprogramming and patterning during amphibian limb regeneration

Neff

King

Mescher

2011

Developmental Dynamics

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A central feature of epimorphic regeneration during amphibian limb regeneration is cellular dedifferentiation. Two questions are discussed. First, what is the origin and nature of the soluble factors involved in triggering local cellular and tissue dedifferentiation? Secondly, what role does the key stem cell transcription factor Sall4 play in reprogramming gene expression during dedifferentiation? The pattern of Sall4 expression during Xenopus hindlimb regeneration is consistent with the hypothesis that Sall4 plays a role in dedifferentiation (reprogramming) and in maintaining limb blastema cells in an undifferentiated state. Sall4 is involved in maintenance of ESC pluripotency, is a major repressor of differentiation, plays a major role in reprogramming differentiated cells into iPSCs, and is a component of the stemness regulatory circuit of pluripotent ESCs and iPSCs. These functions suggest Sall4 as an excellent candidate to regulate reprogramming events that produce and maintain dedifferentiated blastema cells required for epimorphic regeneration. Developmental Dynamics 240:979-989,

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“…These proteins are essential for generation and maintenance of the ES cell identity by forming an auto-regulatory network that controls the expression and repression of pluripotency-and differentiation-associated genes respectively (Boyer et al 2005). Although a key pluripotency mediator in ES cells, several studies have reported the expression of OCT4 in unmanipulated and cultured somatic tissues (Zhao et al 2007, Kristensen et al 2010, Pacini et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Pluripotency-associated stem cell marker expression in proliferative cell cultures derived from adult human pancreas

White

Alturaifi²,

Holliman³

et al. 2011

Journal of Endocrinology

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The source of new b-cells in adult human pancreas remains incompletely elucidated with recent studies on rodents providing evidence for neogenesis from progenitor cells in addition to self-replication. The aim of this study was to investigate the expression of pluripotency-associated stem cell markers in proliferative cultures derived from adult human pancreas. Human pancreatic tissue was obtained from deceased donors following ethical approval and relative consent. Isletenriched fraction was separated from the retrieved organ by digestion and density gradient centrifugation. Dissociated cells were seeded in adherent culture forming proliferative 'islet survivor cells' (ISCs). These were characterised at fifth passage by RT-PCR, immunofluorescence staining, FACS, western blot and transfection studies with an OCT4 promoter-driven reporter. Nuclear expression of the pluripotency-associated stem cell marker complex OCT4/SOX2/NANOG was confirmed in ISCs. The phenotype constituted w8% of the overall population. OCT4 biosynthesis was confirmed by western blot and activation of an exogenous OCT4 promoter. Co-expression of pluripotency-associated markers has been confirmed in proliferative primary cells derived from adult human pancreas. Further studies are required to elucidate whether these cells possess functional stem cell characteristics and assess potential for differentiation into pancreatic cell lineages including new b-cells.

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Constitutive Expression of Pluripotency-Associated Genes in Mesodermal Progenitor Cells (MPCs)

Cited by 47 publications

References 33 publications

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Dedifferentiation and the role of sall4 in reprogramming and patterning during amphibian limb regeneration

Pluripotency-associated stem cell marker expression in proliferative cell cultures derived from adult human pancreas

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