Constitutive expression of the AHR signaling pathway in a bovine mammary epithelial cell line and modulation by dioxin-like PCB and other AHR ligands

Symposium review: Immunological detection of the bovine conceptus during early pregnancy

“…Aryl hydrocarbon receptor and products of its activation, including CYP1A1 and PPARG, were detected in human placenta (Storvik et al, 2014). Bovine mammary parenchymal cells and cultured blood lymphocytes express AHR (Girolami et al, 2015), and our unpublished results show abundant expression of AHR in endometrial immune and parenchymal cells during early pregnancy in dairy heifers (T. L. Ott and M. Hartzell, Pennsylvania State University, unpublished data). We hypothesize that IDO1 increases tryptophan metabolites, including kynurenine, resulting in activation of the AHR to induce mediators of immune tolerance.…”

Modulation of aflatoxin B1 cytotoxicity and aflatoxin M1 synthesis by natural antioxidants in a bovine mammary epithelial cell line

“…Sufficient cDNA was prepared in a single run to perform the q-PCR experiments for all the selected genes. Primers for CYP1A1, GSTA1, GSTA2, NQO1, UGT1A1, UGT1A6, and GAPDH were from Girolami et al, (2015), whereas primers for CYP2A13, CYP3A28, CAT, EPXH1, EPXH2, EPXH3, EPXH4, GSTM1, GPx, and SOD were designed on Bos Taurus GenBank and Ensembl mRNA sequences using Primer 3 Software (version 3.0, Applied Biosystems, Foster City, CA). Oligonucleotides were designed to cross the exon/exon boundaries to minimize the amplification of contaminant genomic DNA and were analysed with the NetPrimer tool (available at http://www.premierbiosoft.…”

Tissue distribution and characterization of feline cytochrome P450 genes related to polychlorinated biphenyl exposure

“…Since DL-PCBs have been known as an inducer for the AHR-CYP1 signaling pathway (Girolami et al, 2015;Larigot et al, 2018), the relationship between the TEQ of DL-PCBs (CB77 and CB118) and CYP mRNA expression in the liver was investigated. The relative mRNA expression levels of CYP1A1 and CYP1B1 revealed the significant positive correlations with CB77 and the total TEQ.…”