Constitutive over-expression of rice chymotrypsin protease inhibitor gene OCPI2 results in enhanced growth, salinity and osmotic stress tolerance of the transgenic Arabidopsis plants

Tiwari, Lalit Dev; Mittal, Dheeraj; Mishra, Ratnesh Chandra; Grover, Anil

doi:10.1016/j.plaphy.2015.03.012

Cited by 26 publications

(23 citation statements)

References 22 publications

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“…Similarly, a Bowman-Birk type proteinase inhibitor (BBI) was shown to be responsible for increasing salt tolerance in wheat (Shan et al, 2008). Moreover, overexpression of rice OCPI2 was found to enhance the salt tolerance in transgenic Arabidopsis under the CAM35S promoter (Tiwari et al, 2015). Our results are consistent with the above reports, in which expression of the PI gene was shown to be upregulated under a constitutive promoter in salt-stressed conditions.…”

Section: Molecular Analysissupporting

confidence: 91%

“…However, transgenics at lower concentrations showed earlier growth than at higher concentrations. A similar phenotypic response was noted in transgenic plants overexpressing the rice chymotrypsin proteinase inhibitor (OCPI2) (Tiwari et al, 2015) and a tobacco PI (NtPI) (Srinivasan et al, 2009).…”

Section: Response Of T1 Progenies To Salt Stresssupporting

confidence: 58%

“…Overall, these results suggest that salt tolerance in transgenic lines was improved under salt stress conditions. In a recent study, a similar increase in proline content was observed in OCPI2-overexpressing transgenic plants under high salt stress, which was correlated with higher transcript expression of pyrroline-5-carboxylate synthase and lowered levels of proline dehydrogenase genes (Tiwari et al, 2015). In another report, Kavas et al (2016) found that transgenic tobacco lines overexpressing the salt-tolerant gene (TasTRG) had significantly higher proline contents when compared to control plants under salt stress conditions.…”

Section: Response Of T1 Progenies To Salt Stressmentioning

confidence: 53%

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Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Rehman¹,

Jørgensen²,

Rasmussen³

et al. 2017

Turk J Biol

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Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better growth, higher chlorophyll content, higher proline contents, and higher average fresh weight when compared to the wild type. This comparative analysis of transgenic and wild-type seedlings allows us to suggest that PI-II gene activity can be upregulated via the OsRGLP2 promoter under osmotic stress conditions.

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Section: Molecular Analysissupporting

confidence: 91%

Section: Response Of T1 Progenies To Salt Stresssupporting

confidence: 58%

Section: Response Of T1 Progenies To Salt Stressmentioning

confidence: 53%

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Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Rehman¹,

Jørgensen²,

Rasmussen³

et al. 2017

Turk J Biol

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“…These plates were kept for recovery at 22°C, 16 h light/8 h dark cycles. Salt and osmotic stress were performed according to Tiwari et al (2015).…”

Section: Stress Treatmentsmentioning

confidence: 99%

AtHsc70‐1 negatively regulates the basal heat tolerance in Arabidopsis thaliana through affecting the activity of HsfAs and Hsp101

2020

Self Cite

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Summary Heat shock protein 70 (Hsp70) chaperones are highly conserved and essential proteins with diverse cellular functions, including plant abiotic stress tolerance. Hsp70 proteins have been linked with basal heat tolerance in plants. Hsp101 likewise is an important chaperone protein that plays a critical role in heat tolerance in plants. We observed that Arabidopsis hsc70‐1 mutant seedlings show elevated basal heat tolerance compared with wild‐type. Over‐expression of Hsc70‐1 resulted in increased heat sensitivity. Hsp101 transcript and protein levels were increased during non‐heat stress (HS) and post‐HS conditions in hsc70‐1 mutant seedlings. In contrast, Hsp101 was repressed in Hsc70‐1 over‐expressing plants after post‐HS conditions. Hsc70‐1 showed physical interaction with HsfA1d and HsfA1e protein in the cytosol under non‐HS conditions. In transient reporter gene analysis, HsfA1d, HsfA1e and HsfA2 showed transcriptional response on the Hsp101 promoter. HsfA1d and HsfA2 transcripts were at higher levels in hsc70‐1 mutant compared with wild‐type. We provide genetic evidence that Hsc70‐1 is a negative regulator affecting HsfA1d/A1e/A2 activators, which in turn regulate Hsp101 expression and basal thermotolerance.

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“…At the gene expression level, salinity stress signaling responses and their subsequent impact on growth are modulated by transcription factors from the NAC transcription factors: NAM, ATAF1 and 2, CUC2 (Tran et al 2010;Nakashima et al 2012), DREB/CBF (Dehydration-Responsive Element Binding/C-repeat Binding Factor) (Akhtar et al 2012) and myeloblastosis oncogene homologue (MYB) families (Golldack et al 2014). Engineering salinity tolerance in crops based upon such mechanistic insights has been successful and has received a considerable amount of attention Saibi et al 2015;Tiwari et al 2015;Zhang et al 2015;Hichri et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Genome interrogation for novel salinity tolerant Arabidopsis mutants

Tol

Pinas

Hooykaas

et al. 2016

Plant Cell & Environment

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Soil salinity is becoming an increasingly large problem in agriculture. In this study, we have investigated whether a capacity to withstand salinity can be induced in the salinity sensitive plant species Arabidopsis thaliana, and whether it can be maintained in subsequent generations. To this end, we have used zinc finger artificial transcription factor (ZF-ATFs) mediated genome interrogation. Already within a relatively small collection Arabidopsis lines expressing ZF-ATFs, we found 41 lines that were tolerant to 100 mM NaCl. Furthermore, ZF-ATF encoding gene constructs rescued from the most strongly salinity tolerant lines were indeed found to act as dominant and heritable agents for salinity tolerance. Altogether, our data provide evidence that a silent capacity to withstand normally lethal levels of salinity exists in Arabidopsis and can be evoked relatively easily by in trans acting transcription factors like ZF-ATFs.

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Constitutive over-expression of rice chymotrypsin protease inhibitor gene OCPI2 results in enhanced growth, salinity and osmotic stress tolerance of the transgenic Arabidopsis plants

Cited by 26 publications

References 22 publications

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

AtHsc70‐1 negatively regulates the basal heat tolerance in Arabidopsis thaliana through affecting the activity of HsfAs and Hsp101

Genome interrogation for novel salinity tolerant Arabidopsis mutants

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