Constitutive uptake and degradation of fatty acids by Yersinia pestis

Moncla, Bernard J.; Hillier, Susan; Charnetzky, W T

doi:10.1128/jb.153.1.340-344.1983

Cited by 12 publications

(8 citation statements)

References 22 publications

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“…For each strain, a 9-ml bacterial preculture at the late exponential growth phase (incubation at 28°C for 24 h with no shaking) was used to inoculate a 50-ml liquid growth medium with a concentration of 5 ϫ 10 7 CFU/ml calculated from the optical density at 600 nm. These cultures were incubated for a further 24 h at 28°C with no shaking, allowing the population to reach the early stationary growth phase where the fatty acid composition is rather stable (14,30,41). It was important to use the same growth temperature of 28°C to be able to compare the fatty acids patterns of Y. pestis with those of other Yersinia species computerized in our data base.…”

Section: Bacterial Strains a Total Of 29 Strains Of Y Pestis From Tmentioning

confidence: 99%

High Homogeneity of the Yersinia pestis Fatty Acid Composition

et al. 2000

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The cellular fatty acid compositions of 29 strains ofYersinia pestis representing the global diversity of this species have been analyzed by gas-liquid chromatography to investigate the extent of fatty acid polymorphism in this microorganism. After culture standardization, all Y. pestis strains studied displayed some major fatty acids, namely, the 12:0, 14:0, 3-OH-14:0, 16:0, 16:1ω9cis, 17:0-cyc, and 18:1ω9trans compounds. The fatty acid composition of the various isolates studied was extremely homogeneous (average Bousfield's coefficient, 0.94) and the subtle variations observed did not correlate with epidemiological and genetic characteristics of the strains. Y. pestis major fatty acid compounds were analogous to those found in other Yersiniaspecies. However, when the ratios for the 12:0/16:0 and 14:0/16:0 fatty acids were plotted together, the genus Yersinia could be separated into three clusters corresponding to (i) nonpathogenic strains and species of Yersinia, (ii) pathogenicYersinia enterocolitica isolates, and (iii) Yersinia pseudotuberculosis and Y. pestis strains. The grouping of the two latter species into the same cluster was also demonstrated by their high Bousfield's coefficients (average, 0.89). Therefore, our results indicate that the fatty acid composition ofY. pestis is highly homogeneous and very close to that ofY. pseudotuberculosis.

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Section: Bacterial Strains a Total Of 29 Strains Of Y Pestis From Tmentioning

confidence: 99%

High Homogeneity of the Yersinia pestis Fatty Acid Composition

et al. 2000

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“…It will become apparent in this review that the link between virulence and metabolism is so important in many pathogens that we are probably only seeing the tip of the iceberg. Using the example of the type three secretion system (T3SS), a prototypic virulence determinant of many Gram-negatives, we will show in particular that not only metabolism controls and underpins production and functioning of virulence factors but also that, conversely, virulence factors can modulate metabolic functions of pathogens in a coordinate way, as for example, in Yersinia (Moncla et al, 1983 ; Du et al, 2009 ; Schmid et al, 2009 ) or pave the way for novel metabolic options as in Salmonella (Winter et al, 2010 ) and Pseudomonas (Dacheux et al, 2001b ). The following criteria served as indicators to identify similar phenomena in other pathogens: (i) Can it be assumed or is it known that deletion of a T3SS component results in changes of metabolism?…”

Section: Introductionmentioning

confidence: 99%

“…The cross-talk between virulence functions and metabolism in Y. pestis is presumably even more complicated. Moncla et al ( 1983 ) demonstrated constitutive uptake and degradation of fatty acids in Y. pestis via β–oxidation and the glyoxylate shunt. Intriguingly, fatty acid uptake was found to depend on the T3SS-encoding plasmid pCD1 as well as on the small virulence plasmid encoding the plasminogen activator (Pla) and pesticin (Pst).…”

Section: Introductionmentioning

confidence: 99%

Interrelationship between type three secretion system and metabolism in pathogenic bacteria

Wilharm

Heider

2014

Front. Cell. Infect. Microbiol.

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Before the advent of molecular biology methods, studies of pathogens were dominated by analyses of their metabolism. Development of molecular biology techniques then enabled the identification and functional characterisation of the fascinating toolbox of virulence factors. Increasing, genomic and proteomic approaches form the basis for a more systemic view on pathogens' functions in the context of infection. Re-emerging interest in the metabolism of pathogens and hosts further expands our view of infections. There is increasing evidence that virulence functions and metabolism of pathogens are extremely intertwined. Type three secretion systems (T3SSs) are major virulence determinants of many Gram-negative pathogens and it is the objective of this review to illustrate the intertwined relationship between T3SSs and the metabolism of the pathogens deploying them.

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“…There were many fatty acids metabolites in the present dataset, where deoxycholate, oleic acid, palmitic acid, cholic acid, arachidonic acid, stearic acid and linoleic acid were observed at early timepoints whereas taurodeoxycholic acid, cholic acid, taurocholic acid and glycocholic acid were observed at late time points. Y. pestis is known to utilize palmitic acid as a carbon and energy source (Moncla et al 1983 ) and has enzymes for fatty acid degradation. We studied interaction networks among differentially expressed metabolites, which reveals the interactions between diseases and functions.…”

Section: Resultsmentioning

confidence: 99%

Metabolomic analyses reveal lipid abnormalities and hepatic dysfunction in non-human primate model for Yersinia pestis

et al. 2018

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IntroductionPneumonic plague is caused by the aerosolized form of Yersinia pestis and is a highly virulent infection with complex clinical consequences, and without treatment, the fatality rate approaches 100%. The exact mechanisms of disease progression are unclear, with limited work done using metabolite profiling to study disease progression.ObjectiveThe aim of this pilot study was to profile the plasma metabolomics in an animal model of Y. pestis infection.MethodsIn this study, African Green monkeys were challenged with the highly virulent, aerosolized Y. pestis strain CO92, and untargeted metabolomics profiling of plasma was performed using liquid and gas chromatography with mass spectrometry.ResultsAt early time points post-exposure, we found significant increases in polyunsaturated, long chain fatty acid metabolites with p values ranging from as low as 0.000001 (ratio = 1.94) for the metabolite eicosapentaenoate to 0.04 (ratio = 1.36) for the metabolite adrenate when compared to time-matched controls. Multiple acyl carnitines metabolites were increased at earlier time points and could be a result of fatty acid oxidation defects with p values ranging from as low as 0.00001 (ratio = 2.95) for the metabolite octanoylcarnitine to 0.04 (ratio = 1.33) for metabolite deoxycarnitine when compared to time-matched controls. Dicarboxylic acids are important metabolic products of fatty acids oxidation, and when compared to time matched controls, were higher at earlier time points where metabolite tetradecanedioate has a ratio of 4.09 with significant p value of 0.000002 and adipate with a ratio of 1.12 and p value of 0.004. The metabolites from lysolipids (with significant p values ranging from 0.00006 for 1-oleoylglycerophosphoethanolamine to 0.04 for 1-stearoylglycerophosphoethanolamine and a ratio of 0.47 and 0.78, respectively) and bile acid metabolism (with significant p values ranging from 0.02 for cholate to 0.04 for deoxycholate and a ratio of 0.39 and 0.66, respectively) pathways were significantly lower compared to their time-matched controls during the entire course of infection. Metabolite levels from amino acid pathways were disrupted, and a few from the leucine, isoleucine and valine pathway were significantly higher (p values ranging from 0.002 to 0.04 and ratios ranging from 1.3 to 1.5, respectively), whereas metabolites from the urea cycle, arginine and proline pathways were significantly lower (p values ranging from 0.00008 to 0.02 and ratios ranging from 0.5 to 0.7, respectively) during the course of infection.ConclusionsThe involvement of several lipid pathways post-infection suggested activation of pathways linked to inflammation and oxidative stress. Metabolite data further showed increased energy demand, and multiple metabolites indicated potential hepatic dysfunction. Integration of blood metabolomics and transcriptomics data identified linoleate as a core metabolite with cross-talk with multiple genes from various time points. Collectively, the data from this study provided new insights into th...

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Constitutive uptake and degradation of fatty acids by Yersinia pestis

Cited by 12 publications

References 22 publications

High Homogeneity of the Yersinia pestis Fatty Acid Composition

High Homogeneity of the Yersinia pestis Fatty Acid Composition

Interrelationship between type three secretion system and metabolism in pathogenic bacteria

Metabolomic analyses reveal lipid abnormalities and hepatic dysfunction in non-human primate model for Yersinia pestis

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