Atmospheric co 2 during the Midpiacenzian Warm period and the M2 glaciation Elwyn de la Vega ✉ , thomas B. chalk, Paul A. Wilson, Ratna Priya Bysani & Gavin L. foster The Piacenzian stage of the Pliocene (2.6 to 3.6 Ma) is the most recent past interval of sustained global warmth with mean global temperatures markedly higher (by ~2-3 °C) than today. Quantifying CO 2 levels during the mid-Piacenzian Warm Period (mPWP) provides a means, therefore, to deepen our understanding of Earth System behaviour in a warm climate state. Here we present a new highresolution record of atmospheric CO 2 using the δ 11 B-pH proxy from 3.35 to 3.15 million years ago (Ma) at a temporal resolution of 1 sample per 3-6 thousand years (kyrs). Our study interval covers both the coolest marine isotope stage of the mPWP, M2 (~3.3 Ma) and the transition into its warmest phase including interglacial KM5c (centered on ~3.205 Ma) which has a similar orbital configuration to present. We find that CO 2 ranged from − + 389 8 38 ppm to − + 331 , 11 13 ppm, with CO 2 during the KM5c interglacial being − + 371 29 32 ppm (at 95% confidence). Our findings corroborate the idea that changes in atmospheric CO 2 levels played a distinct role in climate variability during the mPWP. They also facilitate ongoing datamodel comparisons and suggest that, at present rates of human emissions, there will be more CO 2 in Earth's atmosphere by 2025 than at any time in at least the last 3.3 million years.