The study of neutrinos in astrophysics requires the combination of different observational probes. The temperature anisotropies of the cosmic microwave background induced via the kinematic Sunyaev-Zel'dovich (kSZ) effect may provide interesting information since they are expected to receive significant contribution from high-redshift plasma. We present a set of cosmological hydrodynamical simulations that include a treatment of the neutrino component considering four different sum of neutrino masses: Σm ν = (0, 0.15, 0.3, 0.6) eV. Using their outputs, we modelled the kSZ effect due to the large-scale structure after the reionization by producing mock maps, then computed the kSZ power spectrum and studied how it depends on z re and Σm ν . We also run a set of four simulations to study and correct possible systematics due to resolution, finite box size and astrophysics. With massless neutrinos we obtain D kSZ 3000 = 4.0 µK 2 (z re =8.8), enough to account for all of the kSZ signal of D kSZ 3000 = (2.9 ± 1.3) µK 2 measured with the South Pole Telescope. This translates into an upper limit on the kSZ effect due to patchy reionization of D kSZ,patchy 3000 < 1.0 µK 2 (95 per cent confidence level). Massive neutrinos induce a damping of kSZ effect power of about 8, 12 and 40 per cent for Σm ν = (0.15, 0.3, 0.6) eV, respectively. We study the dependence of the kSZ signal with z re and the neutrino mass fraction, f ν , and obtain D kSZ 3000 ∝z re 0.26 (1 − f ν ) 14.3 . Interestingly, the scaling with f ν is significantly shallower with respect to the equivalent thermal SZ effect, and may be used to break the degeneracy with other cosmological parameters. 1 This limit applies in the case of normal hierarchy, for the inverted hierarchy the limit is Σm ν > 0.1 eV. 2012Lesgourgues et al. 2013; Gonzalez-Garcia 2014, and references therein).From the astrophysical point of view, the existence of massive neutrinos influences the evolution of the large-scale structure (LSS) formation via gravitational interaction, thus requiring a generalization of the standard Λ cold dark matter (ΛCDM) cosmological model to account for Σm ν as an additional free parameter, leading to a ΛCDMν scenario. The effect of this new component is twofold. On early times it contributes to the Universe energy budget as radiation, with density of ρ ν = 7 8 4 11where ρ γ is the photon energy density, and N eff is the effective number of neutrino species (N eff = 3.046, according to the SM). This causes a postponing of the matter-radiation equality for a fixed value of Ω m h 2 (where Ω m is the ratio between the total matter denc 2016 RAS