Background: Electronic health (eHealth) is engrained in the healthcare experience to engage patients across the lifespan. eHealth accessibility and optimization is influenced by lifespan development, as older adults experience greater challenges in accessing and using eHealth compared to their younger counterparts. The eHealth Literacy Scale (eHEALS) is the most popular measure to assess patients' confidence in locating, understanding, evaluating, and acting upon online health information. Currently, however, it is unknown if eHEALS measurement properties support empirical comparisons across the lifespan. Objective: The purpose of this study was to examine the structure of eHEALS scores and the degree of measurement invariance by age among a sample of United States (US) adults. Methods: A Qualtrics panel (N = 830) completed the online survey. The sample was categorized into younger (18-48) and middle-to-older (49-84) age groups. Multi-group exploratory structural equation modeling was conducted to identify and confirm the number of underlying eHEALS factors. A structural equation modeling approach was also conducted to explore the degree of configural (number of factors and items on each factor), pattern (factor loading equivalence), and unique (item measurement error equivalence) measurement invariance between age groups. In the best-fitted 3-factor model, independent t-tests were conducted to examine age group differences in average responses. Results: Young (18-48 years old; N = 411) and old (49-84 years old; N = 419) adults completed the survey. A 3-factor model had the best fit (RMSEA = .06; CFI = .99; TLI = .98), as compared to the 1-factor, 2-factor, and 4-factor models. These 3-factors included eHealth Information Awareness (2 items), Information Seeking (2 Items), and Information and Evaluation (4 items). Pattern invariance was supported with acceptable model fit (RMSEA = .06; X2 = Δ p > .05; CFI = 0). Δ Compared with younger adults, older adults had less confidence in eHealth resource awareness (p < .001), information seeking skills (p < .01), and ability to evaluate and act upon online health information (p < .001). Conclusions: Evidence for measurement invariance across the lifespan is supported by this study. The eHEALS can be used to uniquely assess, monitor, and evaluate self-efficacy of Internet users' eHealth information awareness, information seeking skills, and evaluation/action abilities. This finding will allow health researchers and practitioners identify the eHealth deficits experienced by their respondents, so that timely and relevant trainings can be recommended. Future research is needed to extend the number of eHEALS items to capture the social nature of eHealth and to meet gold standard expectations for factor representation (i.e., 3 items).